Inheritance of Fusarium wilt resistance in flax

Edirisinghe, Priyangi; Wang, Lipu; Young, Lester; Booker, Helen; Rashid, Khalid; Kutcher, Hadley

doi:10.1080/07060661.2023.2265900

Cited by 1 publication

(1 citation statement)

References 15 publications

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“…The screening of a recombinant inbred line (RIL) population from a cross between the moderately resistant cultivar ‘Aurore’ and the susceptible cultivar ‘Oliver’ with two Fol isolates (Edirisinghe et al 2023 ) indicated that resistance was controlled in this cross by two independently inherited recessive genes. Using a doubled haploid population derived from the susceptible cultivar ‘Glenelg’ and the resistant line CRZY8/RA91, Spielmeyer et al ( 1998 ) mapped two quantitative trait loci (QTLs) for Fusarium wilt resistance in flax.…”

Section: Introductionmentioning

confidence: 99%

Fine-mapping of a major locus for Fusarium wilt resistance in flax (Linum usitatissimum L.)

Cloutier,

Edwards,

Zheng

et al. 2024

Theor Appl Genet

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Key message Fine-mapping of a locus on chromosome 1 of flax identified an S-lectin receptor-like kinase (SRLK) as the most likely candidate for a major Fusarium wilt resistance gene. Abstract Fusarium wilt, caused by the soil-borne fungal pathogen Fusarium oxysporum f. sp. lini, is a devastating disease in flax. Genetic resistance can counteract this disease and limit its spread. To map major genes for Fusarium wilt resistance, a recombinant inbred line population of more than 700 individuals derived from a cross between resistant cultivar ‘Bison’ and susceptible cultivar ‘Novelty’ was phenotyped in Fusarium wilt nurseries at two sites for two and three years, respectively. The population was genotyped with 4487 single nucleotide polymorphism (SNP) markers. Twenty-four QTLs were identified with IciMapping, 18 quantitative trait nucleotides with 3VmrMLM and 108 linkage disequilibrium blocks with RTM-GWAS. All models identified a major QTL on chromosome 1 that explained 20–48% of the genetic variance for Fusarium wilt resistance. The locus was estimated to span ~ 867 Kb but included a ~ 400 Kb unresolved region. Whole-genome sequencing of ‘CDC Bethune’, ‘Bison’ and ‘Novelty’ produced ~ 450 Kb continuous sequences of the locus. Annotation revealed 110 genes, of which six were considered candidate genes. Fine-mapping with 12 SNPs and 15 Kompetitive allele-specific PCR (KASP) markers narrowed down the interval to ~ 69 Kb, which comprised the candidate genes Lus10025882 and Lus10025891. The latter, a G-type S-lectin receptor-like kinase (SRLK) is the most likely resistance gene because it is the only polymorphic one. In addition, Fusarium wilt resistance genes previously isolated in tomato and Arabidopsis belonged to the SRLK class. The robust KASP markers can be used in marker-assisted breeding to select for this major Fusarium wilt resistance locus.

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Section: Introductionmentioning

confidence: 99%