Inheritance of malignant melanoma in the melim strain of miniature pigs

Hruban, V.; Horák, Vratislav; Fortýn, K; Hrádecký, Jan; Klaudy, J; Smith, Douglas M.; Reisnerova, H.; Majzlík, I.

doi:10.17221/5739-vetmed

Cited by 16 publications

(7 citation statements)

References 19 publications

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“…Sinclair miniature swine is another hereditary animal model of melanoma [ 21 , 33 , 34 ]. It originated from the Hormel miniature pig that also participated in the establishment of the MeLiM strain [ 23 ]. Thus, the Hormel minipig perhaps brought initial genetic predisposition to melanoma in both Sinclair and MeLiM strains.…”

Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

Planska

Burócziová

Strnádel

et al. 2015

Acta Histochemica et Cytochemica

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Spontaneous regression (SR) of human melanoma is a rare, well-documented phenomenon that is not still fully understood. Its detailed study cannot be performed in patients due to ethical reasons. Using the Melanoma-bearing Libechov Minipig (MeLiM) animals of various ages (from 3 weeks to 8 months) we implemented a long-term monitoring of melanoma growth and SR. We focused on immunohistochemical detection of two important extracellular matrix proteins, collagen IV and laminin, which are associated with cancer. We showed that SR of melanoma is a highly dynamic process. The expression of collagen IV and laminin correlated with changes in population of melanoma cells. Tumours of 3-week-old animals consisted primarily of melanoma cells with a granular expression of collagen IV and laminin around them. Thereafter, melanoma cells were gradually destroyed and tumour tissue was rebuilt into the connective tissue. Collagen IV expression slightly increased in tumours of 10-week-old pigs showing extracellular fibrous appearance. In tumours of older animals, areas lacking melanoma cells demonstrated a low expression and areas still containing melanoma cells a high expression of both proteins. We considered the age of 10 weeks as a turning point in the transition between tumour growth and SR of the MeLiM melanoma.

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Section: Discussionmentioning

confidence: 99%

Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

Planska

Burócziová

Strnádel

et al. 2015

Acta Histochemica et Cytochemica

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“…Later, Ho et al (2010) have shown that the B haplotype actually corresponds to the SLA1 0201 and 0701 alleles. In MeLiM, a three-genes mode of inheritance was proposed by Hruban et al (2004) . More recently, different approaches were used to decipher melanoma occurrence, considering it as a complex trait, as detailed below for the MeLiM swine.…”

Section: Melanoma Inheritance In Pigsmentioning

confidence: 99%

The MeLiM Minipig: An Original Spontaneous Model to Explore Cutaneous Melanoma Genetic Basis

Bourneuf

2017

Front. Genet.

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Melanoma is the deadliest skin cancer and is a major public health concern with a growing incidence worldwide. As for other complex diseases, animal models are needed in order to better understand the mechanisms leading to pathology, identify potential biomarkers to be used in the clinics, and eventually molecular targets for therapeutic solutions. Cutaneous melanoma, arising from skin melanocytes, is mainly caused by environmental factors such as UV radiation; however a significant genetic component participates in the etiology of the disease. The pig is a recognized model for spontaneous development of melanoma with features similar to the human ones, followed by a complete regression and a vitiligo-like depigmentation. Three different pig models (MeLiM, Sinclair, and MMS-Troll) have been maintained through the last decades, and different genetic studies have evidenced a complex inheritance of the disease. As in humans, pigmentation seems to play a prominent role, notably through MC1R and MITF signaling. Conversely, cell cycle genes as CDKN2A and CDK4 have been excluded as predisposing for melanoma in MeLiM. So far, only sparse studies have focused on somatic changes occurring during oncogenesis, and have revealed major cytological changes and a potential dysfunction of the telomere maintenance system. Finally, the spontaneous tumor progression and regression occurring in these models could shed light on the interplay between endogenous retroviruses, melanomagenesis, and adaptive immune response.

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“…Immunological research in the porcine species is of great importance for its direct impact on the veterinary control of pig husbandry as well as for the development of biomedical models (1,2). Melanoblastoma-bearing Libechov Minipigs (MeLiM) spontaneously develop cutaneous melanomas around birth, with clinical and histopathological features comparable to human counterparts (3,4). Animals can carry multiple lesions from benign to highly invasive, eventually leading to lymph nodes and visceral metastasis.…”

Section: Introductionmentioning

confidence: 99%

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model

et al. 2020

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Immunological research in pigs benefits from many improvements with a direct impact on the veterinary control of pig husbandry and on biomedical models. We compiled the available knowledge to develop gating strategies to monitor simultaneously all blood immune cell types by multicolor flow cytometry in Melanoblastoma-bearing Libechov Minipigs (MeLiM). The MeLiM pig spontaneously develops cutaneous melanomas that regress few months later. We monitored lymphoid and myeloid cell subsets in 3 to 21 weeks old pigs. Interestingly, neutrophils, type III monocytes (CD163 + CD14 + MHC II −) and CD4 − CD8α − T cells are less abundant in oldest animals in contrast to eosinophils, type II monocytes (CD163 − CD14 low MHC II +), B cells, γδ T cells, CD4 + CD8α + and CD4 − CD8α + T cells. Melanoma occurrence led to changes in the blood cell composition. Higher proportions of NK cells, CD4 + and CD4 + CD8α + T cells, and CD21 − B cells among B cells are found in young melanoma-bearing piglets, consistent with the immune-mediated spontaneous regression in the MeLiM model.

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Inheritance of malignant melanoma in the melim strain of miniature pigs

Cited by 16 publications

References 19 publications

Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig

The MeLiM Minipig: An Original Spontaneous Model to Explore Cutaneous Melanoma Genetic Basis

The Composition of Circulating Leukocytes Varies With Age and Melanoma Onset in the MeLiM Pig Biomedical Model

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