Inheritance of Resistance to Downy Mildew (Peronospora destructor [Berk.] Casp.) from Allium roylei Stearn in the Backcross Allium cepa L. × (A. roylei ×A. cepa)

Kofoet, A.; Kik, C.; Wietsma, W. A.; Vries, J. N. de

doi:10.1111/j.1439-0523.1990.tb00467.x

Cited by 53 publications

(20 citation statements)

References 5 publications

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“…Vegetable germplasm developed at Cornell are generally released or licensed nonexclusively to maximize use of these materials by public and private researchers. Resistance to downy mildew caused by Peronospora destructor was identified in A. roylei as two weakly linked genes, Pd1 and Pd2, and has been transferred into cultivated onion (De Vries et al, 1992a;Kofoet et al, 1990;Scholten et al, 2007). This transfer is seen as a large step in reducing fungicide use and subsequent environmental impacts (Scholten et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Onion (Allium cepa L.) Lines and Hybrids Possessing Bs1 for Resistance against Multiple Isolates of Botrytis squamosa

et al. 2015

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Onion (Allium cepa L.) lines resistant to Botrytis leaf blight (BLB) were produced by backcrossing the Bs1 BLB resistance of Allium roylei Stearn into cultivated onion. This study evaluated important issues for the commercial use of BLB‐resistant onions: the relative degree of BLB control possible when Bs1 is homozygous rather than heterozygous, the control achieved against multiple pathogen isolates, and the potential for negative traits associated with transfer of Bs1. Homozygous BLB‐resistant onions tested in inoculated growth chamber screens with five distinct Botrytis squamosa Walker isolates and in naturally infested regional field trials showed significantly lower BLB symptoms than susceptible commercial hybrid controls. F1 hybrids heterozygous for the Bs1 gene showed levels of BLB symptoms between those of the homozygous resistant and susceptible controls, confirming previous reports of partial dominance. The difference in BLB control in plants heterozygous for Bs1 compared with homozygous plants was more noticeable under heavier disease pressure. Hybrids heterozygous for Bs1 had similar level of BLB control in most of the field trials as their parental homozygous BLB‐resistant lines, indicating that either heterozygous or homozygous BLB resistant hybrids could be used commercially for BLB control. There were no differences in the degree of pathogenicity across the five B. squamosa isolates, which were genetically different based on polymorphism for DNA markers, or among the naturally occurring pathogens present at the six different field locations used. No obvious association was noted between presence of the Bs1 resistance gene with unfavorable characteristics such as reduced bulb size or yield.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Onion (Allium cepa L.) Lines and Hybrids Possessing Bs1 for Resistance against Multiple Isolates of Botrytis squamosa

et al. 2015

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“…Kofoet et al ., 1990) . The F2 plants exposed to leaf blight in the present investigation originated from an earlier study of downy mildew resistance segregation .…”

Section: Plant Materialsmentioning

confidence: 99%

“…For this reason, these BC, individuals will also have a higher chance to be leaf blight resistant compared to BC, plants morphologically similar to onion . In the study on downy mildew (Kofoet et al ., 1990), the fraction of resistant individuals among the BC, plants resembling the F 1 was also significantly larger than in the category of BC, plants similar to onion . Hence, breeding leaf blight and downy mildew resistant onion varieties seems feasible using Table 4.…”

Section: Inheritance Of Leaf Blight Resistancementioning

confidence: 99%

Introgression of leaf blight resistance from Allium roylei Stearn into onion (A. cepa L.)

Vries¹,

Wietsma²,

Vries³

1992

Euphytica

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“…As resistance to pests and diseases is often not present in the crop species itself, introduction of these traits from crossable wild relatives can be a solution [3, 4]. For onion, downy mildew resistance is an example of a trait that was identified in A. roylei Stearn [5] and successfully introgressed in onion cultivars [6]. In A. roylei resistance to Botrytis squamosa [7, 8], and Colletotrichum gloeosporioides [9] was discovered as well.…”

Section: Introductionmentioning

confidence: 99%

SNP-markers in Allium species to facilitate introgression breeding in onion

et al. 2016

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BackgroundWithin onion, Allium cepa L., the availability of disease resistance is limited. The identification of sources of resistance in related species, such as Allium roylei and Allium fistulosum, was a first step towards the improvement of onion cultivars by breeding. SNP markers linked to resistance and polymorphic between these related species and onion cultivars are a valuable tool to efficiently introgress disease resistance genes. In this paper we describe the identification and validation of SNP markers valuable for onion breeding.ResultsTranscriptome sequencing resulted in 192 million RNA seq reads from the interspecific F1 hybrid between A. roylei and A. fistulosum (RF) and nine onion cultivars. After assembly, reliable SNPs were discovered in about 36 % of the contigs. For genotyping of the interspecific three-way cross population, derived from a cross between an onion cultivar and the RF (CCxRF), 1100 SNPs that are polymorphic in RF and monomorphic in the onion cultivars (RF SNPs) were selected for the development of KASP assays. A molecular linkage map based on 667 RF-SNP markers was constructed for CCxRF. In addition, KASP assays were developed for 1600 onion-SNPs (SNPs polymorphic among onion cultivars). A second linkage map was constructed for an F2 of onion x A. roylei (F2(CxR)) that consisted of 182 onion-SNPs and 119 RF-SNPs, and 76 previously mapped markers. Markers co-segregating in both the F2(CxR) and the CCxRF population were used to assign the linkage groups of RF to onion chromosomes. To validate usefulness of these SNP markers, QTL mapping was applied in the CCxRF population that segregates for resistance to Botrytis squamosa and resulted in a QTL for resistance on chromosome 6 of A. roylei.ConclusionsOur research has more than doubled the publicly available marker sequences of expressed onion genes and two onion-related species. It resulted in a detailed genetic map for the interspecific CCxRF population. This is the first paper that reports the detection of a QTL for resistance to B. squamosa in A. roylei.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0879-0) contains supplementary material, which is available to authorized users.

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Inheritance of Resistance to Downy Mildew (Peronospora destructor [Berk.] Casp.) from Allium roylei Stearn in the Backcross Allium cepa L. × (A. roylei ×A. cepa)

Cited by 53 publications

References 5 publications

Evaluation of Onion (Allium cepa L.) Lines and Hybrids Possessing Bs1 for Resistance against Multiple Isolates of Botrytis squamosa

Evaluation of Onion (Allium cepa L.) Lines and Hybrids Possessing Bs1 for Resistance against Multiple Isolates of Botrytis squamosa

Introgression of leaf blight resistance from Allium roylei Stearn into onion (A. cepa L.)

SNP-markers in Allium species to facilitate introgression breeding in onion

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