Inheritance of resistance to Verticillium wilt in Wisconsin

Hunter, D. E.; Darling, H. M.; Stevenson, F. J.; Cunningham, Charles E.

doi:10.1007/bf02862864

Cited by 33 publications

(21 citation statements)

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“…LD tests that take into consideration presence of subpopulations greatly reduce the number of false positive associations and thus lead to more reliable results. Hunter et al (1968) investigated inheritance of Verticillium resistance in tetraploid potatoes. They reported that the resistance is heritable, and their results indicate that a genetic mechanism of resistance is based predominantly on additive genes.…”

Section: Discussionmentioning

confidence: 99%

“…Two weeks after planting, the plants were inoculated by adding 10 ml of spore suspension to each of three holes made 3 cm from the base of the stem and penetrating into the soil through the root zone. Reaction of the plants was rated 4 weeks after inoculation on a scale from 1 to 5 according to Hunter et al (1968): (1) no disease Table 1 Tetraploid potatoes used for the linkage disequilibrium mapping. Division into subpopulations is based on degree of relatedness estimated from coancestry matrix (as described in "Materials and methods") Subpopulation A Subpopulation B Subpopulation C Unknown pedigree symptoms, (2) slight wilting and unilateral discoloration of lower leaves, (3) moderate wilting involving more than one-half of the plant, (4) severe wilting involving more than one-half of the plant, and (5) plant dead from wilt ( Fig.…”

Section: Disease Screeningmentioning

confidence: 99%

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Linkage disequilibrium mapping of a Verticillium dahliae resistance quantitative trait locus in tetraploid potato (Solanum tuberosum) through a candidate gene approach

et al. 2003

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We have used the linkage disequilibrium mapping method to test for an association between a candidate gene marker and resistance to Verticillium dahliae in tetraploid potato. A probe derived from the tomato Verticillium resistance gene ( Ve1) identified homologous sequences ( StVe1) in potato, which in a diploid population map to chromosome 9, in a position analogous to that of the tomato resistance gene. When a molecular marker closely linked (1.5 cM) to the homologues was used as a candidate gene marker on 137 tetraploid potato genotypes (mostly North American cultivars), the association between the marker and resistance was confirmed ( P<0.001). The amount of phenotypic variation in resistance explained by the allele of the STM1051 marker was greater than 10% and 25% in two subpopulations that were inferred from coancestry data matrix. Cloning of homologues from the highly resistant potato cv. Reddale indicates that the resistance quantitative trait locus (QTL) comprises at least an eleven-member family, encoding plant-specific leucine-rich repeat proteins highly similar to the tomato Ve genes. The sequence analysis shows that all homologues are uninterrupted open reading frames and thus represent putative functional resistance genes. This is the first time that the linkage disequilibrium method has been used to find an association between a resistance gene and a candidate gene marker in tetraploid potato. We have shown that it is possible to map QTL directly on already available potato cultivars, without developing a new mapping population.

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Section: Discussionmentioning

confidence: 99%

Section: Disease Screeningmentioning

confidence: 99%

Linkage disequilibrium mapping of a Verticillium dahliae resistance quantitative trait locus in tetraploid potato (Solanum tuberosum) through a candidate gene approach

et al. 2003

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“…Ratings were made on July 12, July 29 and Sept. 8; and the mean of the three ratings was calculated. The reaction of the plants to Verticillium infection was rated on Sept. 8 and Sept. 10 on a scale from 1 to 5 (Hunter et al 1968): (1) no disease symptoms, (2) slight wilting and unilateral discoloration of lower leaves, (3) moderate wilting involving more than one-half of the plant, (4) severe wilting involving more than one-half of the plant, and (5) plant dead from wilt.…”

Section: Test Of Haplotype Association and Genetic Linkage Analysismentioning

confidence: 99%

“…At present, however, the most widely grown North American potato cultivars are not resistant to Verticillium wilt, though relatively good resistance can be found in some breeding lines (Simko et al 2004b). The resistance observed in tetraploid (2n=4x=48) cultivated potato (Solanum tuberosum L.) appears to be polygenic in nature, and is based predominantly on the additive effect of minor genes (Hunter et al 1968). The recently identified StVe1 locus, which provides partial resistance against V. dahliae, maps to the short arm of chromosome 9 (Simko et al 2004a), a position analogous to that of the tomato Ve resistance gene (Diwan et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Mapping genes for resistance to Verticillium albo-atrum in tetraploid and diploid potato populations using haplotype association tests and genetic linkage analysis

et al. 2004

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Verticillium wilt disease of potato is caused predominantly by Verticillium albo-atrum and V. dahliae. StVe1 -a putative QTL for resistance against V. dahliae -was previously mapped to potato chromosome 9. To develop allele-specific, SNP-based markers within the locus, the StVe1 fragment from a set of 30 North American potato cultivars was analyzed. Three distinct and highly diverse haplotypes can be distinguished at the StVe1 locus. These were detected in 97%, 33%, and 10% of the cultivars analyzed. We tested for haplotype association and for genetic linkage between the StVe1 haplotypes and resistance of tetraploid potato to V. albo-atrum. Moreover, field resistance was assessed in diploid populations with known molecular linkage maps in order to identify novel QTLs. Resistance QTLs against V. albo-atrum were detected on four chromosomes (2, 6, 9, and 12) at the diploid level, with one QTL on chromosome 2 contributing over 40% to the total phenotypic variation of the trait. At the tetraploid level, a significant association between the StVe1-839-C haplotype and susceptibility to the disease was detected, suggesting that resistance-related genes directed against V. albo-atrum and V. dahliae are located in the same genomic region of chromosome 9. However, on the basis of the present analysis, we cannot determine whether these genes are closely linked or if a single gene provides resistance against both Verticillium species. To assess the usefulness of the StVe1-839-C haplotype for marker-assisted selection, we subjected the resistance data to Bayesian analysis, and calculated positive (0.65) and negative (0.75) predictive values, and overall predictive accuracy (0.72). Our results indicate that tagging of additional genes for resistance to Verticillium with molecular markers will be required for efficient marker-assisted selection.

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“…Verticillium wilt resistance in potato is heritable (Akeley et al, 1956;Hunter et al, 1968;Corsini et al, 1990;Hoyos et al, 1993;Concibido et al, 1994), and appears to be stable over time (Corsini et al, 1985;Hoyos et al, 1991). Some resistance to Verticillium exists in cultivated Solanum germplasm.…”

Section: Introductionmentioning

confidence: 98%

Identification of potato interspecific hybrids resistant toVerticillium wilt and determination of criteria for resistance assessment

Jansky

Rouse

2000

Potato Res

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SummaryVerticillianl dahliae causes potato (Solarium tuberosam) early dying disease. Interspecific hybrids containing S. tuberosunl, S. berthaaltii, S. bukasovii, S. chacoense, S. gourlayi, S. sparsipilum and S. tarijense were identifed with resistance to V. dahliae. Clones were grown in replicated trials for three years in a plot containing approximately 50 colony forming units (cfu) V. dahliae per g soil. Clones were evaluated for vine maturity, early dying symptoms and stem colonization. Five resistant 2x clones were identified with early maturity and low levels of stem colonization. A single maturity score and one disease score, each obtained in mid-August, were sufficient to distinguish among clones. An effective threshold level for considering a stem to be infected was determined to be 25 cfu/100 ial sap. Early dying symptoms did not correlate strongly with stem colonization. Numerous stems must be sampled when making resistance assessments. The average cfu/100 IJI sap correlated strongly with the number of infected stems.

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Inheritance of resistance to Verticillium wilt in Wisconsin

Cited by 33 publications

References 2 publications

Linkage disequilibrium mapping of a Verticillium dahliae resistance quantitative trait locus in tetraploid potato (Solanum tuberosum) through a candidate gene approach

Linkage disequilibrium mapping of a Verticillium dahliae resistance quantitative trait locus in tetraploid potato (Solanum tuberosum) through a candidate gene approach

Mapping genes for resistance to Verticillium albo-atrum in tetraploid and diploid potato populations using haplotype association tests and genetic linkage analysis

Identification of potato interspecific hybrids resistant toVerticillium wilt and determination of criteria for resistance assessment

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