Inheritance of Resistance to Zucchini Yellow Mosaic Virus in Watermelon

Guner, Nihat; Rivera-Burgos, Luis A.; Wehner, Todd C.

doi:10.21273/hortsci13169-18

Cited by 9 publications

(7 citation statements)

References 22 publications

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“…Interestingly, some of the accessions that were found to be resistant to WMV and PRSV-W in previous studies also had some level of resistance to ZYMV (Guner et al, 2018b;Strange et al, 2002). The PI accession 595203 was found to be resistant to PRSV-W by Strange et al (2002), to the Florida strain of ZYMV (ZYMV-FL) by Boyhan et al (1992), and to the Chinese strain of ZYMV (ZYMV-CH) and WMV by Xu et al (2004); it was the most resistant to ZYMV according to the germplasm screening and retest studies by Gillaspie and Wright (1993).…”

Section: Resultsmentioning

confidence: 93%

“…Worldwide, more than 10 viruses are known to be limiting factors for commercial watermelon production (Romay et al, 2014;Wang et al, 2017). The major viruses affecting watermelon in the United States are Zucchini yellow mosaic virus (ZYMV), Papaya ringspot virus-watermelon strain (PRSV-W, formerly Watermelon mosaic virus-1), and Watermelon mosaic virus (WMV, formerly Watermelon mosaic virus-2) (Ali et al, 2012;Chen et al, 2008;Guner et al, 2018aGuner et al, , 2018bMorata and Puigdom enech, 2017;Wehner et al, 2001). These viruses are difficult to control because they are spread by insect feeding.…”

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confidence: 99%

“…They reported that resistance to ZYMV-CH was conferred by a single recessive gene, for which the symbol zym-CH was suggested. A single recessive gene (zym-FL) was found to control the high level of resistance to the ZYMV-FL strain in PI 595203 (Guner et al, 2018b). The eIF4E gene was demonstrated to be associated with the ZYMV-FL resistance identified in PI595203, and molecular markers have been developed for use in cultivar development (Ling et al, 2009).…”

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confidence: 99%

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Screening for Resistance to Zucchini yellow mosaic virus in the Watermelon Germplasm

Guner¹,

Pesic‐VanEsbroeck²,

Rivera-Burgos³

et al. 2019

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The Florida strain of Zucchini yellow mosaic virus (ZYMV-FL) is one of the major viral diseases of watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai]. Although some screening has been performed for ZYMV resistance in watermelon, the germplasm collection has not been screened extensively for resistance. The objectives of this study were to screen the U.S. Department of Agriculture (USDA) watermelon germplasm collection for ZYMV resistance and to verify the disease rating for the most resistant and most susceptible accessions. We used a randomized complete block with four replications, 1613 PI accessions, and 41 cultivars. ‘Charleston Gray’ and ‘Crimson Sweet’ susceptible controls were used to verify that the ZYMV inoculum was virulent. After the last rating, an enzyme-linked immunosorbent assay (ELISA) was performed to determine the presence of a virus in the plant tissue. The PI accessions with high resistance to ZYMV-FL that also exhibited resistance to other watermelon viruses were PI 595203, PI 386015, PI 386016, PI 386024, PI 386025, PI 386026, PI 244018, PI 244019, PI 485583, PI 494528, and PI 494529. The ZYMV-FL retest of the most resistant 46 PI accessions showed that there were some escapes. Sixteen resistant PI accessions had a rating of 3.0 or less for the average and maximum ratings: PI 595203, PI 537277, PI 560016, PI 386016, PI 386019, PI 485580, PI 494529, PI 595200, PI 494528, PI 595201, PI 386025, PI 494530, PI 386015, PI 386021, PI 386026, and PI 596662. Overall, PI 595203 had the highest resistance according to both the germplasm screening and the retest studies.

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Section: Resultsmentioning

confidence: 93%

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confidence: 99%

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confidence: 99%

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Screening for Resistance to Zucchini yellow mosaic virus in the Watermelon Germplasm

Guner¹,

Pesic‐VanEsbroeck²,

Rivera-Burgos³

et al. 2019

horts

Self Cite

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“…Botswana Guner, 2004;Guner and Wehner, 2008;Guner et al, 2019 PI 494528 Citrullus mucosospermus (Fursa) Fursa Nigeria Provvidenti, 1986;Provvidenti, 1991;Boyhan et al, 1992;Guner 2004;Guner and Wehner, 2008;Guner et al, 2019 PI 494529 C. mucosospermus (Fursa) Fursa Nigeria Boyhan et al, 1992;Guner, 2004;Guner et al, 2019 PI 537277 Citrullus colocynthis (L.) Schrad. Pakistan Guner, 2004;Guner et al, 2019 PI 560016 C. mucosospermus (Fursa) Fursa Nigeria Guner, 2004;Guner and Wehner, 2008; Guner, 2004;Guner and Wehner, 2008;Guner et al, 2019 PI 595203 C. mucosospermus (Fursa) Fursa Nigeria Boyhan et al, 1992;Xu et al, 2004;Guner, 2004;Ling et al, 2006;Guner and Wehner, 2008;Guner et al, 2018 C. amarus Schrad. South Africa Guner, 2004;Guner and Wehner, 2008; symptom severity measured in different leaves and absorbance in the ZYMV assay using the fourth leaf were much smaller and sometimes not significant.…”

Section: Resultsmentioning

confidence: 99%

Response of Some Watermelon Accessions to a Puerto Rico Strain of Zucchini yellow mosaic virus

Rosa-Valentín¹,

Wessel-Beaver²,

Rodrigues³

2020

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One of the most important members of the Potyviridae is Zucchini yellow mosaic virus (ZYMV). It affects watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] as well as other cucurbits in most parts of the world. Although several genotypes have been reported as having resistance to ZYMV, differential responses to ZYMV strains are known to occur. Using a Puerto Rico strain of ZYMV (ZYMV-PR, GenBank accession number MN422959), we tested the response of 11 genotypes [PIs from the U.S. Department of Agriculture (USDA) National Genetic Resources Program] previously reported as having resistance to this virus. In two greenhouse trials, the first three leaves of seedlings of each genotype were mechanically inoculated with ZYMV-PR. An enzyme-linked immunosorbent assay (ELISA) was done on each seedling’s fourth leaf and symptom severity was rated on the first, third, fifth, and seventh leaves. There were significant genotype × trial interactions for most variables, but some genotypes performed consistently in both trials. All seedlings of PI 537277 tested negative for ELISA (absorbance < 0.200) across both trials. PI 537277, PI 595200, PI 595201, and PI 595203 were generally among the accessions with the lowest symptom severity scores. Overall, symptom severity correlated poorly with ELISA readings. But all plants of PI 537277, and most plants of PI 595201 and PI 595203, had low ELISA readings and low severity scores. Despite having low severity scores, PI 595200 was among the genotypes with the highest ELISA readings in trial 2. For the plant breeder, the most useful genotypes are those that exhibit reduced severity as well as low ELISA. PI 537277, PI 595201, and PI 595203 met those criteria in this study. Of these three accessions, PI 595203 would be the most useful in a breeding program because it has shown resistance to the Puerto Rico, Florida, and China strains of ZYMV.

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“…The accession PI 595203, also known as Egun, shows high resistance to ZYMV-CH, with total absence of viral symptoms, and controlled by a single recessive gene, zym-CH [135]. This accession is also highly resistant to the Florida strain ZYMV-FL [134], with this resistance being controlled by a single recessive gene, zym-FL [136]. At present, it is not known whether both zym-CH and zym-FL are the same gene.…”

Section: Watermelon (Citrullus Lanatus [Thunb] Matsum and Nakai) And Citrullus Sppmentioning

confidence: 99%

Natural Resistances to Viruses in Cucurbits

Martín-Hernández

Picó

2020

Agronomy

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Cucurbit viruses cause considerable economic losses worldwide. The most common viral diseases affecting this crop family are Potyviruses, Cucumoviruses, Criniviruses, Ipomoviruses, Tobamoviruses, and the emerging Begomoviruses. Four main cucurbit crops are grown worldwide, namely melon, cucumber (Cucumis), watermelon (Citrullus), and squash (Cucurbita). Huge natural variation is also available within each genus, providing valuable sources of genetic resistance to these diseases. Intraspecific and intrageneric diversity and crossability are key factors to select the optimum breeding strategies. Melon and cucumber are diverse species for which intraspecific resistance is available. Conversely, in Citrullus and Cucurbita, wild relatives provide the resistance diversity absent in watermelon and in C. pepo. Some of the classical sources used by breeders, many of which are multi-resistant, come from corresponding origin centers in Asia, Africa, and America, as well as from secondary centers of diversity. Genetic studies have identified dominant and recessive and often complex resistance. Many of the genes identified have been mapped and markers for MAS are available, but higher mapping resolutions are required to identify the corresponding genes. Only a few genes could be cloned and functionally characterized. Efforts are underway to use genome mapping and functional genomics to advance toward a genomic-assisted breeding against viral diseases in cucurbits.

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Inheritance of Resistance to Zucchini Yellow Mosaic Virus in Watermelon

Cited by 9 publications

References 22 publications

Screening for Resistance to Zucchini yellow mosaic virus in the Watermelon Germplasm

Screening for Resistance to Zucchini yellow mosaic virus in the Watermelon Germplasm

Response of Some Watermelon Accessions to a Puerto Rico Strain of Zucchini yellow mosaic virus

Natural Resistances to Viruses in Cucurbits

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