Inheritance of very high glucosinolate content in Ethiopian mustard seeds

Abstract

Seed meal amendments rich in glucosinolates are of interest for soil pest and disease control. The Ethiopian mustard (Brassica carinata A. Braun) line N2-6215, with very high levels of seed glucosinolates (160 lmol/g), was developed from the line C-101 (116 lmol/g) following mutagenesis. The objective of this research was to study the inheritance of very high seed glucosinolate content. Plants of N2-6215 were reciprocally crossed with plants of the line C-101. The F 1 , F 2 , and BC 1 F 1 plant generations were evaluated in two environments and seeds from individual plants were analysed for total glucosinolate content. The very high glucosinolate content in N2-6215 seeds was largely subject to maternal control. No cytoplasmic effects were detected. The trait was found to be oligogenic and determined by at least two or three genes. The estimates of broad-sense heritability were 0.45 and 0.58 in both environments, whereas the estimates of narrowsense heritability were 0.35 and 0.50. The moderate heritability and oligogenic control of the trait suggest the feasibility of breeding for increased seed glucosinolate content in Ethiopian mustard.

Alterations in the leaf lipidome of Brassica carinata under high-temperature stress

“…Its ability to reduce summer weed species’ seed banks makes it suitable for integrated pest management in crop production systems [ 7 , 16 ]. Carinata also has potential as a feed crop (due to its high protein and low fiber content [ 17 – 19 ]) and biofumigant (varieties with high glucosinolate content [ 20 ]). There have been concerted efforts both from public and private entities in the last few years to establish carinata as a sustainable biofuel crop in the United States [ 1 , 16 , 21 – 24 ].…”

Modification of Leaf Glucosinolate Contents in Brassica oleracea by Divergent Selection and Effect on Expression of Genes Controlling Glucosinolate Pathway

“…Although the estimates of heritability could not be calculated with the experimental design used in our work, according to the results obtained, we can conclude that heritability should be high enough. In this sense, Madsen et al ( 2014 ) in B. napus and Márquez-Lema et al ( 2009 ) in B. carinata , estimated the heritability for total GSLs in seeds with values of h 2 = 0.90 and h 2 = 0.58, respectively. In another study, Van Doorn et al ( 1998 ) established the heritability for two aliphatic GSLs (SIN and PRO) in different cultivars of Brussels sprouts with values of h 2 = 0.77 and h 2 = 0.79, respectively.…”

Brassica carinata: Biology and agronomy as a biofuel crop

“…Carinata shoots comprise mostly of aliphatic GSLs with roots containing aliphatic and aromatic GSLs at about a 1:1 ratio and ranged from 8 to 25 μmol g −1 of total GSLs (Kirkegaard & Sarwar, 1998). Seeds contain about 80 μmol g −1 of GSLs comprising mainly of sinigrin, but can be as high as 160 μmol g −1 (Marquez‐Lema et al, 2008; Mnzava & Olsson, 1990; Seepaul et al, 2020; Seepaul, Small, Mulvaney, et al, 2019). After oil extraction, carinata seed meal can be used as supplemental protein for animal feed since it contains up to 53.5% crude protein, 76% of which are rumen degradable protein (Ban et al, 2017; Paula et al, 2019).…”