Inherited retinal degeneration and apoptosis in mutant zebrafish

Daly, Frank J.; Sandell, Julie H.

doi:10.1002/(sici)1097-0185(20000201)258:2<145::aid-ar4>3.3.co;2-7

Cited by 9 publications

(2 citation statements)

References 8 publications

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“…Another possibility is the compensation of the high ratio of proliferation in zebrafish with apoptosis. Nevertheless, previous data suggest no significant cell death in fish retina (Hoke and Fernald 1998;Julian et al 1998;Daly and Sandell 2000;Li et al 2000).…”

Section: Flow Cytometry Analysismentioning

confidence: 84%

Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species

Cid

Velasco

Ciudad

et al. 2002

Cell and Tissue Research

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In the present study, a descriptive and quantitative analysis of all the proliferating cell populations present in the normal adult retina of three cyprinid species (goldfish, zebrafish, and tench) is reported. Evaluation of cell proliferation was performed in proliferating cell nuclear antigen (PCNA)-labeled tissue sections as well as in single-cell suspensions analyzed by flow cytometry. Our results show that the neural progenitors of the inner nuclear layer (INL) of cyprinids continue dividing in adulthood in uninjured retinas. These cells are probably related to the generation of rods in normal retinal growth, as well as in the production of any retinal cell type in regenerating processes. The distributions of both these cells and their presumptive progeny, the rod precursors, differ from one species to another, being homogeneous in zebrafish, displaced to the periphery in goldfish and to the temporal pole in tench. With regard to the cell apposition at the retinal periphery, it seems to be symmetrical in goldfish and zebrafish, based on a homogeneous extension of the peripheral growth zone (PGZ), but asymmetrical in tench, where it presents a significantly lower extension in the ventral retina. The flow cytometry analyses indicate that, overall, the proportion of proliferating cells is significantly greater in zebrafish retina despite the fact that body growth rate is lower in zebrafish than the other two species.

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Section: Flow Cytometry Analysismentioning

confidence: 84%

Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species

Cid

Velasco

Ciudad

et al. 2002

Cell and Tissue Research

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show abstract

“…In addition, several mutant zebrafish with specific types of retinal degeneration (Neuhauss et al 1999;Daly and Sandell 2000) show apoptosis in the retina and may, therefore, serve as important animal models for degenerative retinal diseases (cf. mammalian models for retinal degeneration; Chen et al 1996).…”

Section: Introductionmentioning

confidence: 99%

Onset and time course of apoptosis in the developing zebrafish retina

Biehlmaier

Köhler

2001

Cell and Tissue Research

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In mammalian development, apoptosis spreads over the retina in consecutive waves and induces a remarkable amount of cell loss. No evidence for such consecutive waves has been revealed in the fish retina so far. As the zebrafish is of growing importance as a model for retinal development and for degenerative retinal diseases, we examined the onset and time course of apoptosis in the developing zebrafish retina and in adult fish. We found that apoptosis peaked in the ganglion cell layer (GCL) and inner nuclear layer (INL) in early developmental stages (3-4 days post-fertilization; dpf) followed by a second, but clearly smaller wave at 6-7dpf. Apoptosis in the outer nuclear layer (ONL) started at 5dpf and peaked at 7dpf. This late-onset high peak of apoptosis of photoreceptors is different from that of all other species examined to date. With 1.09% of cells in the GCL and 1.10% in the ONL being apoptotic, the rate of apoptosis in the developing zebrafish retina was conspicuously lower than that observed in other vertebrates (up to 50% in GCL). During development (2-21dpf), apoptotic waves were most obvious in the central retina, whereas in the periphery near the marginal zone (MZ), apoptosis was much lower; in adult animals, practically no apoptosis was present in the central retina but it still occurred near the MZ. Our data show that the onset and time course of apoptosis in the GCL and INL of the zebrafish is comparable with other vertebrates; however, the amount of apoptosis is clearly reduced. Thus, apoptosis in the zebrafish retina may serve more as a mechanism for the fine tuning of the retinal neuronal network after mitotic waves during development or in remaining mitotic areas than as a mechanism for eliminating large numbers of excess cells.

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Modulation of cell proliferation in the embryonic retina of zebrafish (Danio rerio)

Ochocinska

et al. 2000

Dev. Dyn.

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We describe light‐microscopically the development of the embryonic zebrafish eye with particular attention to cell number, cell proliferation, and cell death. The period from 16 to 36 hr post fertilization (hpf) comprises two phases; during the first (16–27 hpf) the optic vesicle becomes the eye cup, and during the second (27–36 hpf) the eye cup begins to differentiate into the neural retina and pigmented epithelium. All cells in the eye primordium are proliferative prior to 28 hpf, and the length of the cell cycle has been estimated to be 10 hr at 24–28 hpf (Nawrocki, 1985). Our cell counts are consistent with that estimate at that age, but not at earlier ages. A 10‐hr cell cycle predicts that the cell number should increase by 7% per hr, but during 16–24 hpf the cell number increased by only 1.5% per hr. Despite the low rate of increase, all cells labeled with bromo‐deoxyuridine, so all were proliferative. We considered three possible explanations for the nearly‐constant cell number in the first phase: proliferation balanced by cell emigration from the eye, proliferation balanced by cell death, and low proliferation caused by a transient prolongation of the cell cycle. We excluded the first two, and found direct support for the third. Previous examinations of the cell cycle length in vertebrate central nervous system have concluded that it increases monotonically, in contrast to the modulation that we have shown. Modulation of the cell cycle length is well‐known in flies, but it is generally effected by a prolonged arrest at one phase, in contrast to the general deceleration that we have shown. © 2000 Wiley‐Liss, Inc.

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Inherited retinal degeneration and apoptosis in mutant zebrafish

Cited by 9 publications

References 8 publications

Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species

Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species

Onset and time course of apoptosis in the developing zebrafish retina

Modulation of cell proliferation in the embryonic retina of zebrafish (Danio rerio)

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