Inherited stress-induced hypertension associates with altered gene expression of thermosensitive TRP ion channels in hypothalamus

Voronova, Irina P.; Tuzhikova, Anna A.; Markel, Arkadij L.; Козырева, Т. В.

doi:10.5455/jeim.021015.or.136

Cited by 13 publications

(14 citation statements)

References 43 publications

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“…Attention is drawn to the rather high expression of the TRPV2 gene, tens, or even hundreds of times higher, than the expression of other TRP ion channels under study. This feature of TRPV2 was also noted in the brain structures [22,25]. This is apparently connected with the participation of this ion channel in the basic processes of vital activity of cells of different specializations.…”

Section: Resultsmentioning

confidence: 64%

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TRPM8 ion channel in neuro-immune regulation of antigen binding function of spleen cells

Tv¹,

Ls²,

Gm³

et al. 2019

Trends Med

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Ion channels, localized in cells with different specialization, may participate in the performance of various functions. In the experiments on rats, quantitative PCR has shown that spleen cells express the genes of thermosensitive TRP ion channels (TRPV1, TRPV2, TRPV3, TRPV4, TRPM8, and TRPA1). Experiments in vitro with the addition of an agonist of the TRPM8 ion channel menthol (10 -5 M and 10 -8 M) to the incubation medium indicate the presence of the TRPM8 on immune cells, and its stimulatory effect on the antigen binding, the number of rosette-forming cells doubles under the influence of menthol at concentration of 10 -8 M. Stimulation of the TRPM8 ion channel localized in the skin nerve afferents by menthol (1% L-menthol) also has a stimulating effect on the antigen-binding function of spleen cells without interfering with the additional stimulation of this function when exposed to the TRPM8 agonist directly on spleen cells. The obtained results testify to the important role of the TRPM8 ion channel of different localization (in spleen cells and in the peripheral sensory neurons) in the regulation of the antigen binding function in the spleen. This indicates new moments of the neuro-immune interaction.

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Section: Resultsmentioning

confidence: 64%

“…Quantitative RT-PCR. The expression of the ion channel genes was verified through the quantitative RT-PCR method, described in detail earlier [22]. This method gives us the possibility to investigate different genes the expressions of which are varied significantly.…”

Section: Spontaneous Rosettesmentioning

confidence: 99%

TRPM8 ion channel in neuro-immune regulation of antigen binding function of spleen cells

Tv¹,

Ls²,

Gm³

et al. 2019

Trends Med

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“…TRPV4 has also been shown to play a role in systemic osmolality sensing and regulation. In fact, the channel can be activated by small changes in osmotic pressure (35), and its overexpression was observed in the SON of the hypothalamus of inherited stress-induced arterial hypertensive rats (62). The observation of an impaired release of vasopressin in TRPV4knockout mice following hypertonic stress demonstrated the involvement of the channel in vasopressin-release mechanisms (34).…”

Section: Cardiovascular Function Of Trpv4mentioning

confidence: 98%

“…TRPV4 is a nonselective cation channel of the vanilloid subfamily of TRP channels and is broadly expressed in a wide range of tissues including the brain (4,8,17,31,41,(53)(54)(55)62) and cardiovascular system (18). It functions as a calcium permeable channel activated directly or indirectly by various kinds of stimuli, such as hypoosmolarity, warm temperature, low pH, mechanical stimulation, synthetic agonists, and endogenous chemicals such as arachidonic acid and neurotransmitter signals (1,9,34,35,41,57,63,71), and plays a multitude of functional roles in both physiological and pathological conditions.…”

Section: Cardiovascular Function Of Trpv4mentioning

confidence: 99%

Trpv4 involvement in the sex differences in blood pressure regulation in spontaneously hypertensive rats

Makiko

Yamanaka

Miyamoto

et al. 2018

Physiological Genomics

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Arterial pressure (AP) is lower in premenopausal women than in men of a similar age. Premenopausal women exhibit a lower sympathetic activity and a greater baroreceptor reflex; however, mechanisms controlling sex differences in blood pressure regulation are not well understood. We hypothesized that different neuronal functions in the cardiovascular centers of the brains of men and women may contribute to the sex difference in cardiovascular homeostasis. Our previous studies on male spontaneously hypertensive rats (SHRs) and their normotensive counterparts, Wistar Kyoto (WKY) rats, revealed that the gene-expression profile of the nucleus tractus solitarius (NTS), a region of the medulla oblongata that is pivotal for regulating the set point of AP, is strongly associated with AP. Thus, we hypothesized that gene-expression profiles in the rat NTS are related to sex differences in AP regulation. Because female SHRs clearly exhibit lower AP than their male counterparts of a similar age, we investigated whether SHR NTS exhibits sex differences in gene expression by using microarray and RT-qPCR experiments. The transcript for transient receptor potential cation channel subfamily V member 4 ( Trpv4) was found to be upregulated in SHR NTS in females compared with that in males. The channel was expressed in neurons and glial cells within NTS. The TRPV4 agonist 4-alpha-phorbol-12,13-didecanoate (4α-PDD) decreased blood pressure when injected into NTS of rats. These findings suggest that altered TRPV4 expression might be involved in the sex differences in blood pressure regulation.

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“…Activation of the TRPM8 ion channel by its agonist menthol contributes to raising blood pressure [23]. Moreover, in hypertensive animals, the expression of the TRMP8 ion channel gene is reduced [24], which can be regarded as a compensatory change. Consequently, the two cold-sensitive ion channels TRMP8 and TRPA1 with respect to the parameters of the cardiovascular system manifest themselves ambiguously.…”

Section: Conflicts Of Interestmentioning

confidence: 99%

Effect of Various Activation of the Skin TRPA1 Ion Channel on Cardiovascular Systemic Parameters in Hypertensive Rats

Tv¹,

Es²

2021

Int Arch Clin Pharmacol

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The effect of different activation of the skin TRPA1 ion channel on the parameters of the cardiovascular system was studied in rats with hereditary stress-induced hypertension while recovery from anesthesia. The activation of the TRPA1 ion channel was performed by application to skin its agonist -allyl isothiocyanate (AITC) in two concentrations 2.5% and 0.5%. The following parameters were measured that characterize the work of the cardiovascular system: Blood pressure (systolic, diastolic, and mean), heart rate, blood flow, and tail blood volume. Stimulation of the TRPA1 ion channel by 2.5% AITC resulted in a diminishing rate of increase in blood pressure and a sharp increase in heart rate while recovering from anesthesia. The mean rate of the arterial pressure rise during recovery from anesthesia was 0.4 ± 0.16 mmHg/min compared to 1.4 ± 0.19 mmHg/ min in control (solvent application), P < 0.01. The heart rate increase was 4.7 ± 0.28 beats/min2 compared to 0.6 ± 0.39 beats/min2 in control (P < 0.001). Parameters of blood flow and the tail blood volume were not significantly changed at 2.5% AITC compared to control. The effect of 0.5% AITC on cardiovascular parameters was another. There was no influence on the recovery of arterial pressure and the heart rate, but there was a pronounced acceleration in the recovery of blood flow and tail blood volume after anesthesia. The mean rate for blood flow recovery at 0.5% AITC application was 0.3 ± 0.07 ml/min2 against 0.1 ± 0.05 ml/min2 in control (P < 0.03) and for the tail blood volume was 1.2 ± 0.33 μl/ min against 0.4 ± 0.14 μl/min in control (P < 0.02). Thus, the influence of the skin TRPA1 ion channel on the parameters of the cardiovascular system depends on the degree of its activation. A low level of the TRPA1 stimulation enhanced the blood flow, while a higher level of the TRPA1 activation discouraged to increase in arterial pressure and significantly increased the heart rate. The results suggest the possibility of the therapeutic use of TRPA1 ion channel activation.

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Inherited stress-induced hypertension associates with altered gene expression of thermosensitive TRP ion channels in hypothalamus

Cited by 13 publications

References 43 publications

TRPM8 ion channel in neuro-immune regulation of antigen binding function of spleen cells

TRPM8 ion channel in neuro-immune regulation of antigen binding function of spleen cells

Trpv4 involvement in the sex differences in blood pressure regulation in spontaneously hypertensive rats

Effect of Various Activation of the Skin TRPA1 Ion Channel on Cardiovascular Systemic Parameters in Hypertensive Rats

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