Inhibin/activin-betaE subunit is expressed in normal and pathological human placental tissue including chorionic carcinoma cell lines

Gingelmaier, Andrea; Brüning, Ansgar; Kimmich, Tanja; Makovitzky, Josef; Bergauer, F.; Schießl, Barbara; Friese, Klaus; Mylonas, Ioannis

doi:10.1007/s00404-009-1340-5

Cited by 17 publications

(18 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Concerning activins B and AB, conflicting studies about their expression in placenta were reported (McCluggage et al, 1998;Petraglia, 1997). The βc and βE subunits were also identified in this organ (Gingelmaier et al, 2011;Weissenbacher et al, 2010).…”

Section: Inhibins and Activinsmentioning

confidence: 99%

The endocrine function of human placenta: an overview

Costa

2016

Reproductive BioMedicine Online

270

173

View full text Add to dashboard Cite

During pregnancy, several tightly coordinated and regulated processes take place to enable proper fetal development and gestational success. The formation and development of the placenta is one of these critical pregnancy events. This organ plays essential roles during gestation, including fetal nourishment, support and protection, gas exchange and production of several hormones and other mediators. Placental hormones are mainly secreted by the syncytiotrophoblast, in a highly and tightly regulated way. These hormones are important for pregnancy establishment and maintenance, exerting autocrine and paracrine effects that regulate decidualization, placental development, angiogenesis, endometrial receptivity, embryo implantation, immunotolerance and fetal development. In addition, because they are released into maternal circulation, the profile of their blood levels throughout pregnancy has been the target of intense research towards finding potential robust and reliable biomarkers to predict and diagnose pregnancy-associated complications. In fact, altered levels of these hormones have been associated with some pathologies, such as chromosomal anomalies or pre-eclampsia. This review proposes to revise and update the main pregnancy-related hormones, addressing their major characteristics, molecular targets, function throughout pregnancy, regulators of their expression and their potential clinical interest.

show abstract

Section: Inhibins and Activinsmentioning

confidence: 99%

The endocrine function of human placenta: an overview

Costa

2016

Reproductive BioMedicine Online

270

173

View full text Add to dashboard Cite

show abstract

“…Moreover, inhibin βE is synthesized in normal and abnormal placenta [5,34,35] and human cervix [36]. This novel subunit was also demonstrated in the Ishikawa endometrial carcinoma cell line, which suggests that these cells may be useful as an in vitro model for studying the βE-subunit [31].…”

Section: Discussionmentioning

confidence: 99%

Evidence of inhibin/activin subunit betaC and betaE synthesis in normal human endometrial tissue

Mylonas

Brüning

Shabani

et al. 2010

Reprod Biol Endocrinol

Self Cite

View full text Add to dashboard Cite

BackgroundInhibins are important regulators of the female reproductive system. Recently, two new inhibin subunits betaC and betaE have been described, although it is unclear if they are synthesized in normal human endometrium.MethodsSamples of human endometrium were obtained from 82 premenopausal, non-pregnant patients undergoing gynecological surgery for benign diseases. Endometrium samples were classified according to anamnestic and histological dating into proliferative (day 1-14, n = 46), early secretory (day 15-22, n = 18) and late secretory phase (day 23-28, n = 18). Immunohistochemical analyses were performed with specific antibodies against inhibin alpha (n = 81) as well as inhibin betaA (n = 82), betaB (n = 82), betaC (n = 74) and betaE (n = 76) subunits. RT-PCR was performed for all inhibin subunits. Correlation was assessed with the Spearman factor to assess the relationship of inhibin-subunits expression within the different endometrial samples.ResultsThe novel inhibin betaC and betaE subunits were found in normal human endometrium by immunohistochemical and molecular techniques. Inhibin alpha, betaA, betaB and betaE subunits showed a circadian expression pattern, being more abundant during the late secretory phase than during the proliferative phase. Additionally, a significant correlation between inhibin alpha and all inhibin beta subunits was observed.ConclusionsThe differential expression pattern of the betaC- and betaE-subunits in normal human endometrial tissue suggests that they function in endometrial maturation and blastocyst implantation. However, the precise role of these novel inhibin/activin subunits in human endometrium is unclear and warrants further investigation.

show abstract

“…Immunofluorescence analysis was performed as previously described (9,(11)(12)(13)(14)(15)23,25). Briefly, cells grown on glass coverslips were fixed with acetone for 10 min at room temperature and washed twice with PBS.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, two additional β-subunits have been identified in humans, βC and βE (7). Although these novel subunits are synthesised in a wide range of normal and malignant tissues (9)(10)(11)(12)(13)(14)(15), their precise function still remains unclear.…”

Section: Introductionmentioning

confidence: 99%

Inhibin-α subunit in normal and malignant human cervical tissue and cervical cancer cell lines

Mylonas

Dian²

2011

Oncol Rep

Self Cite

View full text Add to dashboard Cite

Abstract. Inhibins are dimeric glycoproteins composed of an α-subunit and one of two possible β-subunits (βA or βB), with substantial roles in human reproduction and in endocrineresponsive tumours. Four normal cervical tissue samples together with 10 specimens of well-differentiated squamous cervical cancer and adenocarcinoma of the cervix were immunohistochemically analysed for the expression of the inhibin-α subunit. Additionally, two cervical carcinoma cell lines (HeLa and CaSKi) were analysed with immunofluorescence and RT-PCR for the expression of the inhibin-α subunit. We demonstrated for the first time an immunolabelling of the inhibin-α subunit in normal and malignant cervical tissue, as well as cervical cancer cells. However, the immunoreactive reaction was albeit weak and mostly confined to mitotic cells in the analysed cervical tissues. Additionally, the expression pattern of this subunit was rather inconsistent. Therefore, the immunohistochemical evaluation of this subunit in cervical tissue cannot be used as a prognostic marker as suggested for endometrial cancer. However, since the expression of the inhibin-α subunit is minimal in HeLa cells as assessed by immunofluorescence and RT-PCR, the CaSKi cell line might be a better model for further functional experiments regarding cervical pathogenesis.

show abstract

Inhibin/activin-betaE subunit is expressed in normal and pathological human placental tissue including chorionic carcinoma cell lines

Cited by 17 publications

References 36 publications

The endocrine function of human placenta: an overview

The endocrine function of human placenta: an overview

Evidence of inhibin/activin subunit betaC and betaE synthesis in normal human endometrial tissue

Inhibin-α subunit in normal and malignant human cervical tissue and cervical cancer cell lines

Contact Info

Product

Resources

About