Inhibin/activin subunits beta-A (-βA) and beta-B (-βB) are differentially localised in normal, hyperplastic and malignant human endometrial tissue

Mylonas, Ioannis; Makovitzky, Josef; Hoeing, Anna; Richter, Dagmar; Vogl, Julia; Schulze, Sandra; Jeschke, Udo; Briese, Volker; Friese, Klaus

doi:10.1016/j.acthis.2005.11.002

Cited by 20 publications

(20 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The inhibin-·, -ßA and -ßB subunits were detected in normal human endometria, predominantly localized in the glandular and luminal epithelium, thus confirming previous results (10,11,16). Inhibin-· showed no or minimal expression during the proliferative phase (Fig.…”

Section: Resultssupporting

confidence: 78%

“…While inhibins/activins were initially characterized as endocrine and paracrine hormonal regulators of the hypothalamicpituitary-gonadal axis it is now evident that they are expressed in a wide range of tissues including breast cancer (18,19), the placenta (20)(21)(22), human endometria (5,7,13,16), cultured human endometrial cells (6,12,23), hyperplastic and malignant human endometrial tissue (7)(8)(9)(10)(11)13). We demonstrated a significantly higher inhibin-· expression in the secretory compared to the proliferative endometrial tissue, confirming previous immunohistochemical (6,16) and in vitro results (6,23).…”

Section: Discussionmentioning

confidence: 99%

“…The tumor suppressor activity of the inhibin-· subunit was first identified after the functional deletion of the inhibin-· gene in male and female mice that resulted in primary gonadal sex cord-stromal tumors (15). We recently demonstrated a higher immunohistochemical expression of the inhibin-·, -ßA and -ßB subunits in hyper-plastic endometrial tissue compared to adenocarcinoma, suggesting an involvement of these subunits in endometrial pathogenesis (9)(10)(11). Furthermore, the knowledge of the expression pattern of the inhibin-· subunit in human endometrial tissue can be important, since the preferred secretion form of inhibin or activin is determined by this subunit.…”

Section: Introductionmentioning

confidence: 99%

“…Inhibin has been demonstrated in normal human endometria (5-7) as well as in endometrial hyperplasia and carcinoma (7)(8)(9)(10)(11)(12)(13), although the precise role of inhibin/activin in human endometria still remains unknown. Meanwhile TGF-ß is recognized as a tumor suppressor in the premalignant stages of carcinogenesis with an additional dual role as a pro-oncogene in the later stages of disease leading to metastasis (14).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Expression of the inhibin/activin subunits (-α, -βA and -βB) in normal and carcinogenic endometrial tissue: Possible immunohistochemical differentiation markers

Worbs¹,

Shabani²,

Mayr³

et al. 2007

Oncol Rep

Self Cite

View full text Add to dashboard Cite

Abstract. Inhibins (INH) are dimeric glycoproteins, composed of an alpha-subunit (INH-·) and one of two possible betasubunits (INH-ßA or -ßB), with substantial roles in human reproduction and in endocrine-responsive tumors. The aims of this study were to determine the frequency and tissue distribution of INH-·, -ßA and -ßB in normal and malignant endometria. Samples were obtained from normal (n=46), atrophic (n=8) and endometrioid carcinoma tissue (EC; G1=93; G2=32; G3=14). INH-· was significantly higher in normal compared to malignant endometrial tissue, showing a cyclical variation throughout the menstrual cycle. EC G3 did not express this subunit. INH-ßA and -ßB showed specific staining reactions within the tumor cells. The highest intensity of INH-ßA was observed in the normal secretory phase compared to adenocarcinomas (p<0.05). For INH-ßB, the significantly highest expression was noted in EC G3 compared to EC G2 (p<0.05) and atrophic endometrial tissue. In conclusion, INH-·, -ßA and -ßB were immunolabeled in normal and malignant endometria. INH-· was expressed in a declining relationship in the transition from normal to tumor tissue, suggesting a tumor suppressive function in EC. A high expression of INH-ßB was observed in EC G3 compared to G2, suggesting an important role in the progression of endometrial carcinogenesis. However, the utilization of these subunits as specific tumor markers still remains unclear.

show abstract

Section: Resultssupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Expression of the inhibin/activin subunits (-α, -βA and -βB) in normal and carcinogenic endometrial tissue: Possible immunohistochemical differentiation markers

Worbs¹,

Shabani²,

Mayr³

et al. 2007

Oncol Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although endometrial, prostate and ovarian cancers express these subunits (Mylonas et al 2006a;Risbridger et al 2001;Robertson et al 2007;Worbs et al 2007;Zheng et al 1998), it is quite unclear if and to what extent squamous epithelial cancers also synthesise these novel subunit. While inhibin A, inhibin B and activin A were detected in normal and neoplastic human uterine tissues, including cervical cancer (Petraglia et al 1998), it is still unclear if normal or cancerous cervical epithelial cells as well as cervical cancer cell lines can synthesise the novel inhibin-bE subunit.…”

Section: Introductionmentioning

confidence: 99%

Inhibin/activin-betaE subunit in normal and malignant human cervical tissue and cervical cancer cell lines

et al. 2009

Self Cite

View full text Add to dashboard Cite

Inhibins are dimeric glycoproteins, composed of an alpha-subunit and one of two possible beta-subunits (betaA or betaB), with substantial roles in human reproduction and in endocrine-responsive tumours. Recently a novel beta subunit named betaE was described, although it is still unclear if normal or cancerous cervical epithelial cells as well as cervical cancer cell lines can synthesise the novel inhibin-betaE subunit. About 4 normal cervical tissue samples together with 10 specimens of well-differentiated squamous cervical cancer and adenocarcinoma of the cervix were immunohistochemical analyzed. Additionally, two cervical carcinoma cell lines (HeLa and CaSki) were analyzed by immunofluorescence and RT-PCR for the expression of this novel subunit. We demonstrated for the first time an immunolabelling of the inhibin-betaE subunit in normal and malignant cervical tissue, as well as cervical cancer cells. Although the physiological role is still quite unclear in cervical tissue, inhibin-betaE might play important roles in carcinogenesis. Moreover, the synthesis of this subunit in cervical carcinoma cell lines of squamous and glandular epithelial origins also allows the use of these cell lines in elucidating its functions in cervical cancer pathogenesis. However, since the expression of the inhibin-betaE is minimal in HeLa cells as assessed by immunofluorescence and RT-PCR, the CaSki cell line might be a better model for further functional experiments regarding cervical cancer pathogenesis.

show abstract

Immunoneutralization of endometrial monoclonal nonspecific suppressor factor beta (MNSFβ) inhibits mouse embryo implantation in vivo

Wang

Huang

Nie³

et al. 2007

Molecular Reproduction Devel

View full text Add to dashboard Cite

Successful embryo implantation and pregnancy in mammals depends on the establishment of immune tolerance between the maternal immune system and fetal cells. Monoclonal nonspecific suppressor factor beta (MNSFbeta), a cytokine produced by suppressor T cells in various tissues, possesses an antigen-nonspecific immune-suppressive function, and may be involved in the regulation of the uterine immune response during embryo implantation. In this study, anti-MNSFbeta IgG administered directly into the uterine lumen, significantly inhibited mouse embryo implantation in a dose-dependent manner in vivo, and this effect was reversed by co-administration of recombinant MNSFbeta. The effects of anti-MNSFbeta IgG on the gene pattern profiles in mouse uterine tissues were examined by cDNA microarray and several changes were confirmed by real-time PCR. Anti-MNSFbeta IgG caused up-regulation (> or = 2-fold) of 71 known genes and 17 unknown genes, and decreased expression (> or = 2-fold) of 74 known genes and 43 unknown genes, including several genes previously associated with embryo implantation or fetal development. Most of the known genes are involved in immune regulation, cell cycle/proliferation, cell differentiation/apoptosis, and lipid/glucose metabolism. These results demonstrate that MNSFbeta plays critical roles during the early pregnancy via multiple pathways.

show abstract

Inhibin/activin subunits beta-A (-βA) and beta-B (-βB) are differentially localised in normal, hyperplastic and malignant human endometrial tissue

Cited by 20 publications

References 30 publications

Expression of the inhibin/activin subunits (-α, -βA and -βB) in normal and carcinogenic endometrial tissue: Possible immunohistochemical differentiation markers

Expression of the inhibin/activin subunits (-α, -βA and -βB) in normal and carcinogenic endometrial tissue: Possible immunohistochemical differentiation markers

Inhibin/activin-betaE subunit in normal and malignant human cervical tissue and cervical cancer cell lines

Immunoneutralization of endometrial monoclonal nonspecific suppressor factor beta (MNSFβ) inhibits mouse embryo implantation in vivo

Contact Info

Product

Resources

About