Inhibin and activin-like activity in fluids from male and female gonads: different molecular weight forms and bioactivity/immunoactivity ratios

Grootenhuis, A. J.; Steenbergen, Jacobie; Timmerman, M. A.; Dorsman, Amelia N.R.D.; Schaaper, W.M.M.; Meloen, Rob H.; Jong, Frank H. de

doi:10.1677/joe.0.1220293

Cited by 55 publications

(20 citation statements)

References 26 publications

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“…This contrasts sharply with the situation in follicular fluid where both a&A and a/3-B dimers were found with a number of molecular weight forms of inhibin (Ling et al, 1985;Robertson et al, 1985;Miyamoto et al, 1986;Grootenhuis et al, 1989).…”

Section: Discussioncontrasting

confidence: 86%

“…Sertoli cells were isolated from 21-day-old Wistar rats using a double collagenase digestion method as described by Oonk et al (1985) and Grootenhuis et al (1989 Rat Sertoli cell conditioned medium (rSSCM) was collected every 3 or 4 days for 4-5 weeks and stored at -20°C.…”

Section: Sertoli Ceil Culturementioning

confidence: 99%

“…Bioactive fractions were lyophilized and separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 15% gels (see below). After separation, 2 mm slices were cut and proteins were eluted and methanol precipitated as described by Grootenhuis et al (1989).…”

Section: Purificationmentioning

confidence: 99%

“…For Western blot analysis, separated proteins were electroblotted onto nitrocellulose and incubated with an antiserum against the 22 N-terminal amino acids of the cr-subunit of bovine 32 kDa inhibin, which was described earlier by Grootenhuis et al (1989).…”

mentioning

confidence: 99%

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Inhibin in immature rat Sertoli cell conditioned medium: A 32 kDa αβ-B dimer

Grootenhuis

Timmerman

Hordijk

et al. 1990

Molecular and Cellular Endocrinology

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Section: Discussioncontrasting

confidence: 86%

Section: Sertoli Ceil Culturementioning

confidence: 99%

Section: Purificationmentioning

confidence: 99%

mentioning

confidence: 99%

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Inhibin in immature rat Sertoli cell conditioned medium: A 32 kDa αβ-B dimer

Grootenhuis

Timmerman

Hordijk

et al. 1990

Molecular and Cellular Endocrinology

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“…The interassay and intra-assay coefficients of variation were 13.4 and 8%, respectively. FSH and LH were measured by radioimmunoassay as described by Grootenhuis et al [12], using the antibodies devel oped by Wclschen et al [13]. All results are expressed in terms of NIAADDK-rat FSH-RP-3 and NIAADDK-rat LH-RP-2.…”

Section: Blood Collection and Hormone Assaymentioning

confidence: 99%

Endogenous Reproductive Hormones and Nocturnal Rhythms in Partner Preference and Sexual Behavior of ATD-Treated Male Rats

Bakker

Ophemert²,

Timmerman³

et al. 1995

Neuroendocrinology

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Male rats received subcutaneously silastic capsules, containing the aromatase inhibitor 1,4,6-androstatriene-3,17-dione (ATD), shortly after birth. Control males were given silastic capsules containing cholesterol. The capsules were removed at the age of 21 days. In adulthood, blood serum was collected early and late in the dark phase of the light/dark cycle (experiment I). Testosterone and luteinizing hormone and follicle stimulating hormone (FSH) fluctuated nocturnally, both in ATD and control males, with highest levels late in the dark phase. FSH levels were significantly higher in ATD males. Nocturnal levels of inhibin, a selective suppressor of pituitary FSH secretion, also fluctuated in both ATD and control males, with lowest levels late in the dark phase. In experiment II, ATD and control males were tested for partner preference behavior in a three-compartment box (choice: sexually active male vs. estrous female) early and late in the dark phase. When gonadally intact, ATD males, but not controls, showed a clear nocturnal rhythmicity in partner preference behavior and sexual behavior. Early in the dark phase, such ATD males preferred the vicinity of and interaction with a sexually active male. Late in the dark phase, this preference for the active male shifted to a preference for the estrous female. Control males preferred the estrous female. After castration and subsequent treatment with testosterone via silastic capsules, which ensured constant blood serum levels, ATD males continued to show their nocturnal rhythms in partner preference behavior and in sexual behavior. Thus, the underlying mechanism of the nocturnal rhythmicity phenomenon is an organizational effect of neonatal ATD treatment rather than an activational effect of fluctuating serum hormone levels.

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Immunohistochemical localization and mRNA expression of activin, inhibin, follistatin, and activin receptor in bovine cumulus-oocyte complexes during in vitro maturation

et al. 1998

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The aim of this study was to investigate whether bovine cumulus‐oocyte complexes (COCs) synthesize activin A, inhibin, and follistatin and whether they contain activin receptor during in vitro maturation. Therefore, COCs obtained from small and medium‐sized follicles were cultured in M‐199 supplemented with 10% fetal calf serum (FCS) and gonadotropins for 24 hr. At 0, 6, 12, and 24 hr after the onset of culture, COCs were removed for immunohistochemical staining to detect the expression of activin A, inhibin, follistatin, and activin receptor type II proteins. At 0 and 24 hr, COCs were removed and prepared for reverse‐transcriptase polymerase chain reaction (RT‐PCR) to assess the presence of mRNA of these proteins. It appeared that cumulus cells and oocytes express activin, follistatin, and activin receptor proteins as well as their mRNA. While expression of inhibin mRNA was found exclusively in cumulus cells, the inhibin protein was present in cumulus cells and oocytes. Immunohistochemical study both in cumulus cells and in oocytes often showed a moderate and strong staining intensity for activin and follistatin, respectively. Activin staining underwent little or no change during culture except at 24 hr of maturation, where about 60% of the oocytes showed no staining. Follistatin immunoreactivity remained strong in the majority of COCs. At the onset of culture, a spotlike inhibin staining was observed in the oocyte, which increased after 12 hr and was absent at the end of culture. Activin receptor immunoreactivity in cumulus cell membranes and oolemma increased during oocyte maturation to maximum values at the end of culture in most of the COCs. It is concluded that the consistent presence of activin and the increase in activin receptor in cumulus cells and oocytes during in vitro maturation indicate a paracrine and/or autocrine action for activin on bovine oocyte maturation. This action may be modulated by inhibin and/or follistatin. Mol. Reprod. Dev. 49:186–195, 1998. © 1998 Wiley‐Liss, Inc.

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Inhibin and activin-like activity in fluids from male and female gonads: different molecular weight forms and bioactivity/immunoactivity ratios

Cited by 55 publications

References 26 publications

Inhibin in immature rat Sertoli cell conditioned medium: A 32 kDa αβ-B dimer

Inhibin in immature rat Sertoli cell conditioned medium: A 32 kDa αβ-B dimer

Endogenous Reproductive Hormones and Nocturnal Rhythms in Partner Preference and Sexual Behavior of ATD-Treated Male Rats

Immunohistochemical localization and mRNA expression of activin, inhibin, follistatin, and activin receptor in bovine cumulus-oocyte complexes during in vitro maturation

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