Inhibin concentrations in mares with granulosa cell tumors

Bailey, Michael T.; Troedsson, M.H.T.; Wheaton, J. E.

doi:10.1016/s0093-691x(02)00658-1

Cited by 57 publications

(52 citation statements)

References 8 publications

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“…AMH levels are normal in 93% of other gynecologic and nongynecologic cancers [12]. In mares, E 2 , testosterone and inhibin concentrations also fluctuate widely among mares with GTCTs and are not elevated in all mares with GTCTs [19,31]. On the other hand, AMH concentrations were reportedly higher in all mares with GTCTs compared with cyclic and pregnant mares [32].…”

Section: Discussionmentioning

confidence: 99%

Anti-Müllerian Hormone Profiles as a Novel Biomarker to Diagnose Granulosa-theca Cell Tumors in Cattle

Kitahara

Nambo

Ali

et al. 2012

Journal of Reproduction and Development

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Abstract. This study was carried out to evaluate the blood profile and tissue expression of Anti-Müllerian hormone (AMH) as a biomarker for granulosa-theca cell tumors (GTCTs) in cattle. Five cases with unilateral ovarian GTCTs (GTCT group) were investigated in comparison to other groups of Japanese Black cows, which had either cystic ovarian disease (COD group, n=5), a functional corpus luteum on Days 9 to 11 of the estrous cycle (Day 0=estrus; CL group, n=13) or received superovulation treatment (SOT group, n=13). We used transrectal ultrasonography and measured plasma AMH, estradiol-17β (E 2 ), progesterone (P 4 ) and testosterone (T) levels. Moreover, GTCT tissues were collected and examined by immunohistochemical staining (IHC) for AMH. In the GTCT group, ultrasound images of GTCTs were variable and not definitive. However, the AMH level in the GTCT group (n=3, 58.1 ± 66.3 ng/ml) was significantly higher than in the COD, CL and SOT groups (0.1 ± 0.1 ng/ml for GTCT vs. COD, P<0.05; 0.2 ± 0.1 and 0.3 ± 0.2 ng/ml, respectively for GTCT vs. CL and SOT, P<0.01). The other hormonal levels in the GTCT group had no significant differences compared with the COD or SOT group. Neoplastic granulosa cells labeled with AMH antibody clearly demonstrated a variety of tissue patterns in all cases by IHC. To the best of our knowledge, this is the first study to investigate the blood profile and IHC of AMH in bovine GTCTs. Our findings indicate that AMH may be a novel biomarker to diagnose GTCTs in cattle. Key words: Anti-Müllerian hormone (AMH), Cattle, Granulosa-theca cell tumors (GTCTs), Immunohistochemical staining (J. Reprod. Dev. 58: [98][99][100][101][102][103][104] 2012) G ranulosa-theca cell tumors (GTCTs) are the most common ovarian tumors in cattle [1], and the incidence may be less than 0.5% [2]. GTCTs can affect various breeds [3] and occur at various ages [4] in cattle. GTCTs produce a variety of steroid hormones, causing a subsequent elevation of plasma estradiol-17β (E 2 ), progesterone (P 4 ) and/or testosterone (T) levels [4]. Therefore, a variety of clinical signs, such as nymphomania [5], virilism [6] and mammary gland development [7], could be presented. In contrast, some in GTCTs may not result in abnormal reproductive behavior at all [3]. Preliminary diagnosis of GTCTs can be achieved by transrectal palpation and ultrasonography [1,4]. GTCTs should be suspected if a chronic cystic ovarian disease (COD) does not respond to standard treatment regimens or if the diameter of the ovary is more than 100 mm [1]. Thus, GTCTs should be distinguished from other conditions, such as COD, oophoritis, ovarian abscesses and parovarian cysts, by these clinical signs [8]. However, the distinctions might be difficult depending on the clinical signs and other diagnostic methods, so a definitive diagnosis can only be made based on histopathological examination of the affected ovary [3]. In the treatment of unilateral bovine GTCTs, unilateral ovariectomy is only indicated in cows that do not exhibit alterations in secon...

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Section: Discussionmentioning

confidence: 99%

Anti-Müllerian Hormone Profiles as a Novel Biomarker to Diagnose Granulosa-theca Cell Tumors in Cattle

Kitahara

Nambo

Ali

et al. 2012

Journal of Reproduction and Development

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“…Inhibin α-subunit is considered a useful marker for ovarian cancer in humans and mares [1,5,7,8]. It seems that in the case of equine GTCT the inhibin α-subunit should also be considered as a marker for GTCT affected ovarian follicles.…”

Section: Discussionmentioning

confidence: 99%

Localization of Inhibin .ALPHA.-, .BETA.A- and .BETA.B-Subunits and Aromatase in Ovarian Follicles with Granulosa Theca Cell Tumor (GTCT) in 6 Mares

Hoque

Derar

Senba

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. To clarify the morphological and immunohistochemical characteristics in mares with granulosa theca cell tumor (GTCT), the localization of inhibin subunits (α, βA, βB) and aromatase in the granulosa cell layers and theca layers in the ovarian follicles were determined by immunohistochemical staining. The follicles were obtained from the ovaries of 6 mares with GTCT and 4 normal mares as controls. Immunohistochemically, inhibin α-subunit was localized in the granulosa cells of all follicles showing different sizes in all GTCT cases and βA-subunit was localized in two GTCT cases in all sized follicles. But inhibin βB-subunit and aromatase were not localized in GTCT cases. On the other hand, inhibin α-, βA-, and βB-subunits and aromatase were localized in the large and medium sized follicles, but inhibin βA-and βB-subunits and aromatase were not stained in the small sized follicles in normal cases. These findings suggest that some mares with GTCT can secrete dimeric inhibin (inhibin A), but all GTCT cases cannot secrete inhibin B. By the results of aromatase staining it is clear that testosterone is not converted into estradiol due to the lack of aromatase in the GTCT follicles.

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“…The contralateral ovary is often atrophic. 7,31 The dysgerminoma is an ovarian germ cell tumor; it is the female counterpart of the male seminoma. 7 McEntee 32 summarizes the case of 2 mares affected by ovarian dysgerminomas.…”

Section: Female Reproductive Tract Neoplasiamentioning

confidence: 99%

Reproductive Disorders in Horses

Snider¹

2015

Veterinary Clinics of North America: Equine Practice

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Inhibin concentrations in mares with granulosa cell tumors

Cited by 57 publications

References 8 publications

Anti-Müllerian Hormone Profiles as a Novel Biomarker to Diagnose Granulosa-theca Cell Tumors in Cattle

Anti-Müllerian Hormone Profiles as a Novel Biomarker to Diagnose Granulosa-theca Cell Tumors in Cattle

Localization of Inhibin .ALPHA.-, .BETA.A- and .BETA.B-Subunits and Aromatase in Ovarian Follicles with Granulosa Theca Cell Tumor (GTCT) in 6 Mares

Reproductive Disorders in Horses

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