Inhibin-like material — An immunohistologic marker for prostatic origin of metastases

Sheth, Nandini A.; Doctor, Vatsala M.; Sampat, M. B.; Garde, Seema V.; Arbatti, N.J.; Sheth, A. R.

doi:10.1016/0304-3835(87)90106-6

Cited by 14 publications

(10 citation statements)

References 9 publications

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“…These results are in agreement with other reports (DubC et al, 1987b;Doctor et al, 1986;Abrahamsson et al, 1988;Sheth et al, 1987) and confirm that PSP is detected, by immunohistochemical techniques, only in prostate epithelial cells and, therefore, can be considered to be primarily a prostate organ-specific antigen. However, Ulvsback et al (1989) reported, using Northern blot analysis, identical sized transcripts for PSP9Wbeta-MSP-like proteins in respiratory tissues (trachea, bronchus, and lung) and the antral portion of the gastric mucosa as well as in the prostate.…”

Section: Lrnrnunoperoxidase Stainingsupporting

confidence: 95%

Generation and characterization of monoclonal antibodies to prostate secretory protein

Wright

Huang

Lipford

et al. 1990

Intl Journal of Cancer

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Monoclonal antibodies (MAbs) were produced against a highly purified preparation of prostate secretory protein (PSP) isolated from normal seminal plasma. Fifteen antibodies were selected for further evaluation based on their strong reactivity and specificity for PSP. All the MAbs had a specificity for prostate epithelial cells and none reacted to any of a variety of normal tissues as determined by immunoperoxidase staining. Six of the MAbs were selected for further immunohistochemical evaluation based on their ability to recognize different antigenic determinants. Using competitive binding immunoassays, a variety of overlapping specificities were observed with at least 2 distinct epitopes identified. Although some staining variability was noted, the 6 antibodies, in general, gave the same pattern of tissue reactivity. Both the normal prostate and the benign prostate hyperplastic ductal epithelial cells stained intensely, with 78 to 100% and 50-100% of the cells staining, respectively. The number and often the staining intensity of the tumor cells decreased as the tumor became more undifferentiated. Approximately 40 to 100% and 15 to 70% of the tumor cells stained in the moderately-differentiated and well-differentiated carcinoma tissues, respectively, whereas either no staining was observed or less than 20% of the tumor cells stained in the poorly-differentiated and undifferentiated tumors. Most of the metastatic prostate tumors showed either no staining or scattered staining in a few cells (i.e., less than 20%).

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Section: Lrnrnunoperoxidase Stainingsupporting

confidence: 95%

Generation and characterization of monoclonal antibodies to prostate secretory protein

Wright

Huang

Lipford

et al. 1990

Intl Journal of Cancer

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“…As compared to other prostatic antigens, i.e. prostatic acid phosphatase and prostate specific antigen, we have previously shown that prostatic inhibin peptide appears to be a vital product as it persists in epithelial cells of poorly differentiated prostatic carcinoma when these cells have become negative for the above mentioned antigens (Sheth et al, 1988 The changes in the intensity as well as the pattern observed for FSH and inhibin (Doctor et al, 1986;Sheth et al, 1987) were similar. With this knowledge, it is tempting to speculate that in the prostate FSH plays a regulatory role in inhibin biosynthesis.…”

Section: Nodules and Tumoursmentioning

confidence: 79%

Immunocytochemical localisation of follicle stimulating hormone (FSH) in normal, benign and malignant human prostates

Hurkadli

Ar²,

Sv³

et al. 1990

Br J Cancer

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set at the maximum speed (5 x 1O s at 0C). The receptor preparation was suspended at a concentration of 2 g in 10 ml of assay buffer (0.05 M, Tris-buffer, pH 7.5, containing 0.1 % BSA, 5 mM MgCI2 and 0.1 M sucrose).The testicular receptors were obtained from immature (21-day-old) Holtzman rats (Reichert & Abou-Issa, 1977). The receptor preparations were suspended at a concentration of I g in 10 ml in the assay buffer.Human FSH (hFSH-13) was iodinated by the chloramine-T method (Reichert & Bhalla, 1974) with a specific activity of about 15 tCi yg-'.Binding studies were performed using labelled hFSH (5 ng hFSH in 50 d l assay buffer) in the presence of serial dilutions of unlabelled FSH (I -250 ng) with 50 mg of testicular receptor in 500 gl of assay buffer. The total reaction volume was made up to I ml by addition of assay buffer. The tubes were then incubated in a metabolic shaker at 37°C for 2 h. Incubation was terminated by addition of 2 ml of chilled buffer. The tubes were then centrifuged at 1,500g for 15 min. The supernatant was discarded by decantation and the tubes were drained and counted in the well type gamma-counter. The non-specific binding was determined in presence of a 1000-fold excess of oFSH (NIH-FSH-S-16).

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“…High levels of PIP observed in the prostate-rich secretions of human seminal plasma (HSP) have further substantiated its prostatic origin [ 11. The importance of PIP as a prostatic tissue marker [4] and in confirming the prostatic origin of difficult-to-diagnose invasive tumors in metastic lesions [25] has been demonstrated by immunocytochemical localization studies. These data led us to study the status of immunoreactive PIP in the rat ventral and dorsal prostate and its modulation by steroid and protein hormones.…”

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confidence: 99%

Modulation of Rat Prostatic Inhibinlike Peptide (PIP) by Steroids and Protein Hormones

Teni

Sheth

1988

Archives of Andrology

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Prostatic inhibinlike peptide (PIP) was detected in the ventral but not in the dorsal lobe of rat prostate.On orchiectomy, PIP concentration in the ventral prostate increased significantly, whereas it decreased on testosterone administration and attained value comparable with that in intact control. Estradiol-170-treated animals exhibited high levels of PIP in absence of significant alterations in the protein content. The effect of prolactin and human chorionic gonadotropin on PIP concentration was not so prominent at the dose levels studied. The present study thus demonstrates negative regulation of PIP by testosterone and stimulatory effect of estradiol-170 on PIP in rat ventral prostrate. INTRODUCTIONThere is a high concentration of prostatic inhibinlike peptide (PIP) in the normal and hyperplastic human prostate [4, 23, 301. Biologically active rat PIP has been shown by in vivo [lo] as well as in vitro studies [20]. High levels of PIP observed in the prostate-rich secretions of human seminal plasma (HSP) have further substantiated its prostatic origin [ 11. The importance of PIP as a prostatic tissue marker [4] and in confirming the prostatic origin of difficult-to-diagnose invasive tumors in metastic lesions [25] has been demonstrated by immunocytochemical localization studies. These data led us to study the status of immunoreactive PIP in the rat ventral and dorsal prostate and its modulation by steroid and protein hormones. MATERIALS AND METHODSSteroid and protein hormones used for the study were procured from Sigma Chemicals. Ninety-dayold male rats of Sprague Dawley strain were divided into the following groups: group I, intact controls; group 11, effect of castration; group 111, in vivo effect of steroid hormones: (a) testosterone (T) (500 pg), subgroup contained a minimum of five rats. All the animals except the intact controls were bilaterally castrated. Twenty-four hours after castration, the animals in groups I11 and IV were treated daily with the above-mentioned doses of respective hormones, administered subcutaneously for six days. Three hours after the last injection, animals of all the groups were killed and prostates excised. The dorsal and ventral lobes were weighed separately and homogenized individually in 0.01 M PBS, pH 7.0, and centrifuged at 800 g for 30 min at 4 "C. The supernatants were stored at -20 "C until analyzed for protein by Lowry's Method [14] and PIP by radioimmunoassay (RIA) [29]. A homologous preparation of PIP isolated from HSP [26] was used as a reference standard and as an antigen for radioiodination using Iz5I, obtained from Radiochemical Centre, Amersham, U. K. Iodination was carried out according to the method of Greenwood et al.[6] with appropriate modifications [29]. The specific activity of the labeled hormone ranged from 100 to 150 pCiIpg PIP. Specific antibodies to PIP were raised in rabbit by active immunization. Details regarding the high specificity of this antiserum and RIA methodology have been described [22]. The sensitivity of the assay was 0.7 to 40 ng o...

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Inhibin-like material — An immunohistologic marker for prostatic origin of metastases

Cited by 14 publications

References 9 publications

Generation and characterization of monoclonal antibodies to prostate secretory protein

Generation and characterization of monoclonal antibodies to prostate secretory protein

Immunocytochemical localisation of follicle stimulating hormone (FSH) in normal, benign and malignant human prostates

Modulation of Rat Prostatic Inhibinlike Peptide (PIP) by Steroids and Protein Hormones

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