Inhibin Pro-.ALPHA.C as the Marker of Testicular Function in the Stallion.

Nagata, Shun‐ichi; Nagaoka, Kentaro; Shinbo, Hiromi; Nagamine, Natsuko; Tsunoda, Nobuo; Taniyama, Hiroyuki; Nambo, Yasuo; Groome, Nigel P.; Watanabe, Gen; Taya, Kazuyoshi

doi:10.1262/jrd.46.201

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…The level decreased in transitional and non-breeding seasons. Inhibin is secreted from the testis of stallion [ 12 , 16 ] and it can be the marker of the testicular function [ 13 ]. This clear seasonality in ir-inhibin is supported with previous findings in which increase in number of Sertoli cells and Leydig cell and smooth endoplasmic reticulum per testis has been shown during the breeding season of stallions [ 7 ].…”

Section: Discussionmentioning

confidence: 99%

Annual Changes in Day-length, Temperature, and Circulating Reproductive Hormones in Thoroughbred Stallions and Geldings

Dhakal

Tsunoda

Nakai

et al. 2011

JES

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Changes in follicle-stimulating hormone (FSH), luteinizing hormone (LH), prolactin, immunoreactive(ir)-inhibin, testosterone, estradiol-17β, and insulin-like growth factor (IGF)-I in Thoroughbred stallions along with changes in prolactin secretion in geldings were studied. The correlations of day-length with changes in the concentrations of these hormones were also studied. Five stallions and thirteen geldings were employed to draw blood samples in monthly basis and radioimmunoassay was performed to measure these hormones. All hormones showed a seasonal pattern, the levels being highest during the breeding season and lowest during the winter months. Most of the hormones were at their highest concentration during the month of April, the mid of spring in northern hemisphere. The concentration of circulating IGF-I also demonstrated seasonality, the peak lying on the month of April. The plasma concentration of prolactin also increased during the breeding season. This phenomenon was similar both in stallions and geldings although geldings had lower concentration than that of stallions. The changes in concentration of prolactin in stallions and geldings correlated more towards the day-length than towards the temperature. These results clearly indicate the seasonality of pituitary and gonadal hormones of Thoroughbred stallions, the activity being highest during the month of April and May of the breeding season.

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Section: Discussionmentioning

confidence: 99%

Annual Changes in Day-length, Temperature, and Circulating Reproductive Hormones in Thoroughbred Stallions and Geldings

Dhakal

Tsunoda

Nakai

et al. 2011

JES

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“…The inhibin levels are remarkably higher as compared with those of pregnant and cyclic mares [15][16][17]24] and stallions [33][34][35][36]. The inhibin subunits, namely inhibin ␣ and inhibin/ activin ␤ A and ␤ B subunits, were mainly localized to the interstitial cells of fetal ovaries from mid-to late gestation.…”

Section: Discussionmentioning

confidence: 99%

Localization and Secretion of Inhibins in the Equine Fetal Ovaries1

Tanaka

Taniyama

Tsunoda

et al. 2003

Biology of Reproduction

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To clarify the source of inhibins in equine female fetuses, concentrations of immunoreactive (ir-) inhibin, inhibin pro-alphaC, and inhibin A in both fetal and maternal circulation and in fetal ovaries were measured. In addition, the localization of inhibin alpha and inhibin/activin beta(A), and beta(B) subunits and the expression of inhibin alpha(A) and inhibin/activin beta(A) subunit mRNA in fetal ovaries were investigated using immunohistochemistry and in situ hybridization. Concentrations of circulating ir-inhibin, inhibin pro-alphaC, and inhibin A were remarkably more elevated in the fetal than in the maternal circulation between Days 100 and 250 of gestation. Fetal ovaries contained large amounts of ir-inhibin, inhibin pro-alphaC, and inhibin A. In contrast, these inhibin forms were undetectable in both the maternal ovaries and placenta. The inhibin alpha and inhibin/activin beta(A) and beta(B) subunit proteins were localized to enlarged interstitial cells of the equine fetal ovary. Expression of inhibin alpha and inhibin/activin beta(A) subunit mRNAs were also observed in the interstitial cells. We conclude that the main source of large amounts of inhibins in fetal circulation is interstitial cells of fetal ovary and is not of maternal origin. Furthermore, these inhibins may play some important physiological roles in the development of gonads in the equine fetus.

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“…Precursors of inhibin ␣ subunit have been reported to modulate FSH action (Schneyer et al, 1991) and have been found to play an important role in male reproductive physiology (Tuohimaa et al, 1993;Nagata et al, 2000).…”

Section: Plasma Concentrations Of Inhibin a Inhibin B And Inhibin Pmentioning

confidence: 99%

“…The present finding that inhibin ␣ subunit protein and its mRNA are present in Sertoli cells suggest that the cells may secrete inhibin ␣ subunit precursor, inhibin pro-␣C, and agrees with previous studies in other species (Fujimura et al, 1998;Nagata et al, 1998;Roberts et al, 1989;Kishi et al, 2000;Jin et al, 2001). Precursors of inhibin ␣ subunit have been reported to modulate FSH action (Schneyer et al, 1991) and have been found to play an important role in male reproductive physiology (Tuohimaa et al, 1993;Nagata et al, 2000).…”

Section: Concentrations Of Inhibin a Inhibin B And Inhibin Pro-␣c Imentioning

confidence: 99%

Inhibins in the Male Göttingen Miniature Pig: Leydig Cells Are the Predominant Source of Inhibin B

Wei

Arai

Herath

et al. 2001

Journal of Andrology

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ABSTRACT:The expression of inhibin subunits in the testes of the Göttingen miniature pig was examined by in situ hybridization and immunohistochemistry. In addition, the major forms were determined by enzyme-linked immunosorbent assay (ELISA). Strong positive immunostaining for the inhibin ␣ subunit was observed in Sertoli and late-stage germ cells, but it was weak in Leydig cells. However, Leydig cells showed strong positive staining for the ␤A subunit, but Sertoli cells and spermatogonia showed a weak reaction. Strong positive immunostaining for the ␤B subunit was observed in Leydig cells but spermatogonia showed weak staining for it. In contrast to the staining specificity of inhibin ␣ and ␤A subunits, the ␤B subunit did not exhibit positive staining in Sertoli cells. In situ hybridization revealed that although the ␣ subunit mRNA signal was highly expressed in all cell types, the reaction appeared to be stronger in Sertoli cells and spermatogonia than in Leydig cells. ␤A subunit mRNA expression was somewhat identical to that of the ␣ subunit, however, germ cells showed a weak stain for it. A strong, positive mRNA signal for the ␤B subunit was confined to Leydig cells and late-stage germ cells. ELISA results showed that concentrations of inhibin B and inhibin pro-␣C were high in the circulation and testes. In contrast, inhibin A levels in both plasma and testes were undetectable. The present results strongly suggest that inhibin B is the major form of circulating inhibin and that Leydig cells are the predominant source of this dimeric inhibin in male Göttingen miniature pigs. Furthermore, the germ cells also appear to be an important source of circulating inhibins.

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Inhibin Pro-.ALPHA.C as the Marker of Testicular Function in the Stallion.

Cited by 4 publications

References 24 publications

Annual Changes in Day-length, Temperature, and Circulating Reproductive Hormones in Thoroughbred Stallions and Geldings

Annual Changes in Day-length, Temperature, and Circulating Reproductive Hormones in Thoroughbred Stallions and Geldings

Localization and Secretion of Inhibins in the Equine Fetal Ovaries1

Inhibins in the Male Göttingen Miniature Pig: Leydig Cells Are the Predominant Source of Inhibin B

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