Inhibiting ethylene perception with 1-methylcyclopropene triggers molecular responses aimed to cope with cell toxicity and increased respiration in citrus fruits

Establés-Ortíz, Beatriz; Romero, Paco; Ballester, Ana-Rosa; González-Candelas, Luı́s; Lafuente, Marı́a T.

doi:10.1016/j.plaphy.2016.02.036

Cited by 26 publications

(38 citation statements)

References 36 publications

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“…Simultaneous synthesis and degradation of sucrose during postharvest storage of fruits has been well described (Duque et al, 1999;Zhu et al, 2013). A link between ethylene and sucrose metabolism is suggested by increased gene transcript and enzymatic activity of SPS by exogenous ethylene treatment or during postharvest ripening (Duque et al, 1999;Choudhury et al, 2008;Lombardo et al, 2011). Sucrose treatment of tomato fruits advanced ripening, and increased expression of genes involved in both sugar biosynthesis and degradation .…”

Section: Carbohydrate Metabolismmentioning

confidence: 99%

“…Decreased sucrose accumulation was associated with a down-regulation of two sucrose transporters (SUC11 and SUC12), whose expression is triggered at veraison when grape berries start to accumulate sugars. In citrus, 1-MCP repressed genes involved in starch synthesis and degradation and increased the expression levels of SuSy genes (Estables-Ortiz et al, 2016). In grapes, and another non-climacteric fruit, strawberry, it has been proposed that sucrose functions as a signal that acts upstream of the ABA signaling pathway, thus playing an important role in the regulation of fruit ripening (Jia et al, 2013;Jia et al, 2017).…”

Section: Carbohydrate Metabolismmentioning

confidence: 99%

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Primary Metabolism in Fresh Fruits During Storage

et al. 2020

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The extension of commercial life and the reduction of postharvest losses of perishable fruits is mainly based on storage at low temperatures alone or in combination with modified atmospheres (MAs) and controlled atmospheres (CAs), directed primarily at reducing their overall metabolism thus delaying ripening and senescence. Fruits react to postharvest conditions with desirable changes if appropriate protocols are applied, but otherwise can develop negative and unacceptable traits due to the onset of physiological disorders. Extended cold storage periods and/or inappropriate temperatures can result in development of chilling injuries (CIs). The etiology, incidence, and severity of such symptoms vary even within cultivars of the same species, indicating the genotype significance. Carbohydrates and amino acids have protective/regulating roles in CI development. MA/CA storage protocols involve storage under hypoxic conditions and high carbon dioxide concentrations that can maximize quality over extended storage periods but are also affected by the cultivar, exposure time, and storage temperatures. Pyruvate metabolism is highly reactive to changes in oxygen concentration and is greatly affected by the shift from aerobic to anaerobic metabolism. Ethylene-induced changes in fruits can also have deleterious effects under cold storage and MA/CA conditions, affecting susceptibility to chilling and carbon dioxide injuries. The availability of the inhibitor of ethylene perception 1-methylcyclopropene (1-MCP) has not only resulted in development of a new technology but has also been used to increase understanding of the role of ethylene in ripening of both non-climacteric and climacteric fruits. Temperature, MA/CA, and 1-MCP alter fruit physiology and biochemistry, resulting in compositional changes in carbon-and nitrogen-related metabolisms and compounds. Successful application of these storage technologies to fruits must consider their effects on the metabolism of carbohydrates, organic acids, amino acids and lipids.

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Section: Carbohydrate Metabolismmentioning

confidence: 99%

Section: Carbohydrate Metabolismmentioning

confidence: 99%

Primary Metabolism in Fresh Fruits During Storage

et al. 2020

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“…1-MCP led to membrane and cellular damage, and also to a peel damage disorder called nonchilling peel pitting (NCPP). This disorder is manifested as depressed damaged areas on peel 5,6 ( Supplementary Fig. S1).…”

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confidence: 99%

Insights into the regulation of molecular mechanisms involved in energy shortage in detached citrus fruit

Romero

Alférez

Establés-Ortíz

et al. 2020

Sci Rep

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Harvested fruit undergo carbon and energy deprivation. However, the events underlying this energy-related stress in detached fruit and their involvement in cell damage have not yet been elucidated. We showed that supplementing detached sweet oranges with additional carbon or energy sources reduced peel damage, while inhibitors of energy metabolism increased it. We investigated the effect of an exogenous source of carbon (glycerol), energy (ATP), and an inhibitor of energy metabolism 2-deoxy-D-glucose (DeOGlc) + sodium iodoacetate (IAc), on the transcriptome of harvested fruit flavedo (outer peel part). ATP and Gly induced common, but also specific, alternative modes of energy metabolism by reducing the stress caused by energy shortage. They also induced shifts in energy metabolism that led to the production of the intermediates required for plant defense secondary metabolites to form. ATP and Gly triggered changes in the expression of the genes involved in cell lesion containment through a defined pathway involving hormones and redox-mediated signaling. DeOGlc + IAc had a contrasting effect on some of these mechanisms. These chemicals altered the biological processes related to membrane integrity and molecular mechanisms involving reactive oxygen species (ROS) production, and lipid and protein degradation.

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“…In the present work, we found very marked changes in the genes that encode FAD-dependent oxidoreductases, in abundant GSTs, ABC transporters, and also in different transcription factors, which were only induced in non-conditioned fruits in response to cold stress (Table S1). These genes are regulated in citrus fruits by ethylene (Establés-Ortiz et al, 2016). However, the comparison of the changes in the transcriptome of the flavedo of chilling-exposed fruits with those previously identified in our laboratory when studying the effect of exogenous ethylene (Establés-Ortiz et al, 2016) have shown that the percentage of genes regulated by both chilling and ethylene did not exceed 15% (Establés-Ortiz, 2008).…”

Section: Resultsmentioning

confidence: 99%

“…These genes are regulated in citrus fruits by ethylene (Establés-Ortiz et al, 2016). However, the comparison of the changes in the transcriptome of the flavedo of chilling-exposed fruits with those previously identified in our laboratory when studying the effect of exogenous ethylene (Establés-Ortiz et al, 2016) have shown that the percentage of genes regulated by both chilling and ethylene did not exceed 15% (Establés-Ortiz, 2008). Therefore, this is in line with our previous idea that most protective mechanisms of citrus fruits against chilling do not depend on ethylene.…”

Section: Resultsmentioning

confidence: 99%

Insights into the Molecular Events That Regulate Heat-Induced Chilling Tolerance in Citrus Fruits

2017

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Low non-freezing temperature may cause chilling injury (CI), which is responsible for external quality deterioration in many chilling-sensitive horticultural crops. Exposure of chilling-sensitive citrus cultivars to non-lethal high-temperature conditioning may increase their chilling tolerance. Very little information is available about the molecular events involved in such tolerance. In this work, the molecular events associated with the low temperature tolerance induced by heating Fortune mandarin, which is very sensitive to chilling, for 3 days at 37°C prior to cold storage is presented. A transcriptomic analysis reveals that heat-conditioning has an important impact favoring the repression of genes in cold-stored fruit, and that long-term heat-induced chilling tolerance is an active process that requires activation of transcription factors involved in transcription initiation and of the WRKY family. The analysis also shows that chilling favors degradation processes, which affect lipids and proteins, and that the protective effect of the heat-conditioning treatment is more likely to be related to the repression of the genes involved in lipid degradation than to the modification of fatty acids unsaturation, which affects membrane permeability. Another major factor associated with the beneficial effect of the heat treatment on reducing CI is the regulation of stress-related proteins. Many of the genes that encoded such proteins are involved in secondary metabolism and in oxidative stress-related processes.

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Inhibiting ethylene perception with 1-methylcyclopropene triggers molecular responses aimed to cope with cell toxicity and increased respiration in citrus fruits

Cited by 26 publications

References 36 publications

Primary Metabolism in Fresh Fruits During Storage

Primary Metabolism in Fresh Fruits During Storage

Insights into the regulation of molecular mechanisms involved in energy shortage in detached citrus fruit

Insights into the Molecular Events That Regulate Heat-Induced Chilling Tolerance in Citrus Fruits

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