“Inhibiting inhibitors”: Preliminary results of a new “DNA extraction-amplification” disinhibition technique in critical human samples

Gomes, Cláudia; Alonso, Andoni; Marquina, Domingo; Guardià, Marc; López-Matayoshi, César; Palomo-Díez, Sara; Pérez, Bernardo Perea; Gibaja, Juan Francisco; Arroyo-Pardo, Eduardo

doi:10.1016/j.fsigss.2017.09.066

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Genetic analysis of ancient human remains is challenging due to the loss of genetic material over time, particularly when biological tissues are not perfectly conserved. One of the fields that have contributed most to the genetic analysis of ancient human remains has been forensic genetics (Capelli et al 2003;Palomo-Díez et al 2018;Gomes et al 2019), through the implementation of various extraction techniques adapted to different burial circumstances, and different amplification techniques, including the "inhibition" factor in addition to the factors of the scarcity and fragmentation of genetic material (Caragine et al 2013;Gomes et al 2017;Huang et al 2021). The development of specific software, which takes into account, for example, allelic dropout, is also an important factor to consider when carrying out the statistical interpretation of ancient data.…”

Section: The Genetic Perspectivementioning

confidence: 99%

Family in Medieval Society: A Bioarchaeological Perspective

Gomes,

Curto

2024

Genealogy

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One of the periods with the greatest social, cultural, and religious changes was, without a doubt, the European medieval period. The concept of “Family” was one of the fields that gradually evolved, from individuals who shared the same biological lineage, to members of the same “House”. One of the ways to study the concept of “Family” in ancient periods is through a bioarchaeological perspective, where both anthropology and genetics have proven to be essential disciplines for studying “Families”. Through burial rituals, observing whether the graves were single or multiple, as is carried out in the study of human remains, we discuss the profound contribution of anthropology to the “Family” investigation, through mobility studies, the investigation of biological sex, observing certain congenital anomalies or, even, the study of certain ancient infectious diseases. Concerning genetics, the study of bones or teeth allows us to determine whether individuals were from the same close family or if they belonged to the same lineage through the maternal and paternal sides, being one of the only scientific ways of proposing social relationships between individuals, such as that created through adoption.

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Section: The Genetic Perspectivementioning

confidence: 99%

Family in Medieval Society: A Bioarchaeological Perspective

Gomes,

Curto

2024

Genealogy

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“…and/or from the same individual, must be analyzed according to their state of conservation. The factors that most affect DNA are pH, temperature, humidity, and certain components in the soil (C. Gomes et al 2017;I. Gomes et al 2020).…”

Section: Dna Degradationmentioning

confidence: 99%

Difficulties in Kinship Analysis for Victims’ Identification in Armed Conflicts

et al. 2023

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Regarding human identification in armed conflicts, various complications can be observed. Usually, such difficulties can be social-related, which can include the lack of access to the relative’s genetic material, or the unwillingness of administrative and judicial authorities to participate in the process of identification. In the case of genetics, the analysis allows identifying the individual from a blood sample, a part of an organ, or from skeletal remains, which is why it is considered a much more extensive and effective method when compared with fingerprint techniques or odontology. However, several factors can prevent this identification, such as considerably degraded genetic material. For successful identification, it is mandatory to have access to antemortem biological samples unequivocally attributed to the individual in question, using recombinant nuclear markers, as well as using biological samples from close relatives, whether parents or sons. Nevertheless, the problems associated with armed conflicts make this type of study very difficult. In this article, we focus on the main difficulties encountered when identifying an individual victim of an armed conflict, as well as on the possibilities that exist and on viable measures that could be required to improve the identification of these victims.

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“…To evaluate and compare each reagents effectiveness, we amplified the extracts with nuclear DNA markers (autosomal STRs -auSTRs-, with AmpFLSTR NGM Select, ThermoFisher Scientific®, Foster City, USA, according to users' manual, and X-chromosomal Insertion-Delection polymorphisms -X-InDels-, according to Pereira et al, [4]), using 5 μl of extract volume for each amplification. In the case of mitochondrial DNA (mtDNA), hypervariable regions I and II were amplified, according to [5], method C, evaluating DNA extraction and amplification with an agarose gel (1 g/50 mL TBE 1%).…”

Section: Dna Amplificationmentioning

confidence: 99%

An innovative DNA extraction method: Water versus commercial buffers

Marmol

Moya-Gómez

Gomes

et al. 2019

Forensic Science International: Genetics Supplement Series

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The main objective of DNA extraction is to obtain good quality genetic material in order to carry out its amplification, and corresponding analysis. Most laboratories tend to resort to commercial extraction buffers, which allow a simple and rigorous DNA extraction, with limited handling of the sample, but with high financial cost.As an alternative, we proposed to use water as reagent to extract DNA from blood spots, collected on Whatman® FTA® Cards (Sigma-Aldrich®). We propose to take advantage of hypotonic solutions, in order to obtain a better efficiency in the extraction of genetic material (DNA).On this sense, 219 extractions were performed with Whatman® FTA® Cards (SigmaAldrich®) stained with blood, based on Garcia-Palacios et al., extraction method, with some modifications. Extractions were performed with two types of water (sterile (Lonza™) and distilled (Milli-Q, MilliporeSigma®), as well as, with a commercial extraction kit (Prep-n-Go™, ThermoFisher™ Scientific, Foster City, USA). To evaluate and compare each reagent effectiveness, we amplified DNA extract with mitochondrial DNA Hypervariable regions I and II, and with nuclear DNA markers (autosomal STRs and X-InDels).Our preliminary results suggest that distilled water allows an extraction as effective, or even better, as that obtained with the commercial buffer. These preliminary results offer the possibility for laboratories to choose an inexpensive protocol for DNA extraction, avoiding expensive commercial buffers. Further research is needed to evaluate the performance of each method in blood samples, but also in other biological samples.

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“Inhibiting inhibitors”: Preliminary results of a new “DNA extraction-amplification” disinhibition technique in critical human samples

Cited by 5 publications

References 9 publications

Family in Medieval Society: A Bioarchaeological Perspective

Family in Medieval Society: A Bioarchaeological Perspective

Difficulties in Kinship Analysis for Victims’ Identification in Armed Conflicts

An innovative DNA extraction method: Water versus commercial buffers

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