Inhibiting MMP13 Attenuates Deep Vein Thrombosis in a Mouse Model by Reducing the Expression of Pdpn

Luo, Ji; Zhou, Jin; Luo, Jing-zeng; Wang, Hai-long; Zhao, Xue-ling; Zhou, Ru-dan

doi:10.1007/s11596-024-2862-6

Cited by 1 publication

(1 citation statement)

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“…PDPN plays a diverse range of roles, such as controlling the growth of organs, facilitating cell movement and promoting the formation and spread of tumors [1][2][3][4]. A variety of cell types, including lymphatic endothelial cells [5,6], fibroblasts [7], mesothelial cells [8] and basal cells of stratified epithelia [9], can be identified at the protein and gene level by using several methods such as immunohistochemistry [10] or molecular techniques [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Combining RNAscope, Immunohistochemistry (IHC) and Digital Image Analysis to Assess Podoplanin (PDPN) Protein and PDPN_mRNA Expression on Formalin-Fixed Paraffin-Embedded Normal Human Placenta Tissues

Tomescu,

Cosma,

Fenesan

et al. 2024

CIMB

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The expression and function of podoplanin (PDPN) in the normal human placenta has been debated in placental evaluation. This study emphasizes the importance of a multimodal approach of PDPN expression in normal human placentas. A complete examination is performed using immunohistochemistry, RNAscope and automated Digital Image examination (DIA) interpretation. QuPath DIA-based analysis automatically generated the stromal and histological scores of PDPN expression for immunohistochemistry and RNAscope stains. The umbilical cord’s isolated fibroblasts and luminal structures expressed PDPN protein and PDPN_mRNA. RNAscope detected PDPN_mRNA upregulation in syncytial placental knots trophoblastic cells, but immunohistochemistry did not certify this at the protein level. The study found a significant correlation between the IHC and RNAscope H-Score (p = 0.033) and Allred Score (p = 0.05). A successful multimodal strategy for PDPN assessment in human placentas confirmed PDPN expression heterogeneity in the full-term human normal placenta and umbilical cord at the protein and mRNA level. In placental syncytial knots trophoblastic cells, PDPN showed mRNA overexpression, suggesting a potential role in placenta maturation.

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