Inhibiting wear particles-induced osteolysis with naringin

Abstract

Purpose The purpose of this study was to determine the effects of naringin on osteoclastogenesis and osteolysis both in vitro and in vivo. Methods In this research osteoclasts were generated from mouse bone marrow monocytes with the receptor activator of NF-КB ligand and the macrophage colony stimulating factor. Naringin, at a concentration of 1, 10, 50, and 100 μg/ mL, was respectively added to the medium. Seven days later, the osteoclasts were determined through tartrateresistant acid phosphatase (TRAP) staining. Mature osteoclasts were isolated from newborn rabbits and cultured for three days on bone slices. Naringin at a concentration of 1, 10, 50, and 100 μg/mL was respectively added to the medium. The resorption bone slices were quantified, and the area was calculated after toluidine blue and Mayerhematoxylin staining. Polymethyl methacrylate (PMMA) particles were implanted on the calvariae of C57BL/J6 mice. Naringin, at a dose of 50 μg/kg and 100 μg/kg, was respectively given intraperitoneally for seven. Seven days later, the calvariae were removed and processed for pathological analysis.Results The result indicated that naringin treatment effectively inhibited in vitro osteoclastogenesis and inhibited mature osteoclasts. In vivo data indicated that naringin strongly inhibited PMMA-induced osteolysis. Conclusion Naringin can effectively inhibit osteoclastogenesis and suppress wear particles-induced osteolysis and might be useful in the treatment or prevention of wear particles-induced osteolysis and aseptic loosening for its effect on osteoclast generation and function.

Inhibitory Effects of Lanthanum Chloride on Wear Particle-Induced Osteolysis in a Mouse Calvarial Model

“…Aseptic-loosening-induced failure following arthroplasty surgery greatly influences the long-term survival of a total joint replacement [21]. Although the precise mechanism requires further investigation, the presence of metallic wear particles from total joint implants appears to be associated with significant increases in inflammation and osteoclastogenesis, which are vital and central events in periprosthetic osteolysis [22].…”

Solubility of naringin in ethanol and water mixtures from 283.15 to 318.15 K

“…Naringin (C 27 H 32 O 14 , CAS 10236-47-2) is a double-hydrogen flavonoids compound, its chemical name is naringenine-7-rhamnosidoglucoside (structure is shown in Fig. 1).…”

Are Biologic Treatments a Potential Approach to Wear- and Corrosion-related Problems?

“…These studies can be divided into two classes based on systemic treatment or locally applied treatment. Systemic agents applied to intercede in particle-dependent bone resorption include a spectrum of different agents targeting multiple cellular pathways that alter macrophage, osteoblast, and osteoclast metabolism [16,58]. These agents include estrogen receptor antagonist to block TNF production and luteolin to inhibit TNF, both agents reducing osteolysis [29,42].…”