Inhibition and Reversal of Swelling of Rat Heart Sarcosomes by Reduced Diphosphopyridine Nucleotide

A Reconsideration of the Isolation in Presence of EDTA and the Swelling-Contraction Characteristics of Rat Heart Sarcosomes. Metabolic Topography of the Sarcosomal Contractile Protein*

“…Important information on the nature of sarcosomal swelling (Tapley and Cooper, 1956; Cooper and Tapley, 1957) showed that heart sarcosomes, as compared to liver mitochondria, are very * This investigation was supported by U.S. Public Health Service Research grants from the National Heart Institute (H-6769) and from the National Cancer Institute (CA-05431). A preliminary report of some of the results contained herein has appeared (Arcos et al, 1964b). 1 Abbreviations used in this work: EDTA, ethylenediaminetetraacetate; Tris, tris( hydroxymethyl )aminomethane; NAD (also known as DPN), nicotinamide-adenine dinucleotide; NADH¡ (also known as DPNH), reduced nicotinamideadenine dinucleotide; DNP, 2,4-dinitrophenol.…”