Inhibition by Agaricus blazei Murill Fractions of Cytopathic Effect Induced by Western Equine Encephalitis (WEE) Virus on VERO Cells in Vitro

Sorimachi, Kenji; Ikehara, Yukari; Maezato, Genzo; Ōkubo, Akira; Yamazaki, Sunao; Akimoto, Kazumi; Niwa, Akira

doi:10.1271/bbb.65.1645

Cited by 66 publications

(57 citation statements)

References 8 publications

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“…Zhu et al (20) demonstrated that a sulphated polysaccharide isolated from alga Sargassum patens inhibited up to 96% the formation of plaques in Vero cells (African green monkey kidney cells) infected by HSV-2 (20). Sorimachi et al (19) showed that aqueous extract and fractions obtained by alcoholic extraction of A. blazei Murill were able to inhibit the cytopathic effect of Western Equine Encephalitis (WEE) virus, poliovirus and HSV in cultures of Vero cells (19). The authors demonstrated a greater activity of the alcoholic fractions in comparison to the aqueous extract, however these effect were demonstrated in therapeutic protocol.…”

Section: Discussionmentioning

confidence: 99%

“…Popularly, this fungus is used against physical and mental stress, osteoporosis and gastric ulcer, as stimulatory of immunity and for cholesterol reduction. It is also used as antioxidant, antimutagenic, and anticarcinogenic (5,8,10,12,19). Sorimachi et al (19) also demonstrated that A blazei inhibited the cytopathic effect of Western Equine Encephalitis Virus.…”

Section: Introductionmentioning

confidence: 99%

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Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine herpesviruses in cell culture

Bruggemann¹,

Orlandi²,

Benati³

et al. 2006

Braz. J. Microbiol.

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The aqueous extract of Agaricus blazei Murill ss. Heinem, a basidiomycete native from Brazil, frequently used by popular medicine, mainly in the form of tea, was assessed to its antiviral action against herpes simplex type 1 (HSV-1) and bovine herpes type 1 (BoHV-1) in HEp-2 cell culture. Viral replication inhibition was evaluated by plaque assay and immunofluorescence test. The extract demonstrated virucide action for both viruses, being more effective against HSV-1, inhibiting its infectivity in 78.4 and 73.9% at the concentrations of 50 and 100 µg/mL, respectively moreover, reduction in 47% the number of fluorescent cells was observed for both concentrations. The extract also showed discrete therapeutic activity. These results suggest that A. blazei extract acts mainly in the viral particle, however, the effect during virus replication can not be ruled out.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine herpesviruses in cell culture

Bruggemann¹,

Orlandi²,

Benati³

et al. 2006

Braz. J. Microbiol.

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“…The antitumour effects of AbM components have been documented in mouse models and cancer cell cultures [9][10][11]. AbM mycelium has also been shown to inhibit the cytopathic effects of Western equine encephalitis virus on VERO cells in culture [12], and in vitro bactericidal and fungicidal effects of Agaricus sp. have been reported [13,14].…”

Section: Introductionmentioning

confidence: 99%

An Extract of the Mushroom Agaricus blazei Murill Administered Orally Protects Against Systemic Streptococcus pneumoniae Infection in Mice

Bernardshaw¹,

Johnson²,

Hetland

2005

Scand J Immunol

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The aim was to investigate the antibacterial effect of the biologically active and edible mushroom Agaricus blazei Murill (AbM). A water extract of AbM or PBS control was administered orally before or with challenge to NIH/OlaHsd mice, experimentally infected intraperitoneally with the moderately virulent Streptococcus pneumoniae serotype 6B. End points were bacteraemia and survival rate. The AbM extract, protected against systemic S. pneumoniae 6B infection in the mice. It was most effective when given 24 h before inoculation but did also have protective effects when given together with challenge compared with control. The lack of antibiotic effect on pneumococci in vitro and increased levels of cytokines MIP-2 and TNF-a in the serum of mice receiving AbM extract, indicated that the protective effect of AbM was due to the involvement of the native immune system. This is the first report of anti-infection effects of AbM in vivo. Our results suggest that AbM extract may be useful as additional prophylactic and possibly therapeutic treatment against bacterial and possibly other infections in humans.

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“…Various A. blazei extracts obtained using different solvents have been evaluated and the protective potential of compounds present in these extracts has been the subject of much published research, some by our group (Delmanto et al, 2001;Menoli et al, 2001;Luiz et al, 2003a;Machado et al, 2005;Bellini et al, 2006) and some by other researchers (Sorimachi et al, 2001;Kasai et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

et al. 2008

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Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary cells clone K1 (CHO-K1). The results suggest that all the fractions tested possess cytotoxic and mutagenic potential but no clastogenic effects. Further information is needed on the biochemical components of the A. blazei methanol fractions to identify any substances with cytotoxic and/or mutagenicity potential. These findings indicate that A. blazei methanolic extract should not be used due to their genotoxicity and care should be taken in the use of A. blazei by the general population until further biochemical characterization of this fungi is completed.

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Inhibition by Agaricus blazei Murill Fractions of Cytopathic Effect Induced by Western Equine Encephalitis (WEE) Virus on VERO Cells in Vitro

Cited by 66 publications

References 8 publications

Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine herpesviruses in cell culture

Antiviral activity of Agaricus blazei Murrill ss. Heinem extract against human and bovine herpesviruses in cell culture

An Extract of the Mushroom Agaricus blazei Murill Administered Orally Protects Against Systemic Streptococcus pneumoniae Infection in Mice

Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays

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