Inhibition by Epigallocatechin Gallate of IL-1–Induced Urokinase-Type Plasminogen Activator Expression and Collagen Degradation by Corneal Fibroblasts

Sugioka, Koji; Yoshida, Kōji; Murakami, Junko; Itahashi, Motoki; Mishima, Hiroshi; Nishida, Teruo

doi:10.1167/iovs.19-27306

Cited by 10 publications

(5 citation statements)

References 46 publications

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“…These cytokines are responsible for activating the expression of pro-inflammatory mediators during chronic inflammation. It has been demonstrated that IL1B induces the expression of PLAUR, gene encoding the urokinase plasminogen activator receptor (uPAR), and PLAU (urokinase plasminogen activator; uPA) ( 36 , 37 ). Both PLAUR and PLAU are upregulated in pJIA.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic signatures of classical monocytes reveal pro-inflammatory modules and heterogeneity in polyarticular juvenile idiopathic arthritis

Hounkpe,

Sales,

Ribeiro

et al. 2024

Front. Immunol.

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IntroductionPolyarticular juvenile idiopathic arthritis (pJIA) is a childhood-onset autoimmune disease. Immune cells contribute to persistent inflammation observed in pJIA. Despite the crucial role of monocytes in arthritis, the precise involvement of classical monocytes in the pathogenesis of pJIA remains uncertain. Here, we aimed to uncover the transcriptomic patterns of classical monocytes in pJIA, focusing on their involvement in disease mechanism and heterogeneity.MethodsA total of 17 healthy subjects and 18 premenopausal women with pJIA according to ILAR criteria were included. Classical monocytes were isolated, and RNA sequencing was performed. Differential expression analysis was used to compare pJIA patients and healthy control group. Differentially expressed genes (DEGs) were identified, and gene set enrichment analysis (GSEA) was performed. Using unsupervised learning approach, patients were clustered in two groups based on their similarities at transcriptomic level. Subsequently, these clusters underwent a comparative analysis to reveal differences at the transcriptomic level.ResultsWe identified 440 DEGs in pJIA patients of which 360 were upregulated and 80 downregulated. GSEA highlighted TNF-α and IFN-γ response. Importantly, this analysis not only detected genes targeted by pJIA therapy but also identified new modulators of immuno-inflammation. PLAUR, IL1B, IL6, CDKN1A, PIM1, and ICAM1 were pointed as drivers of chronic hyperinflammation. Unsupervised learning approach revealed two clusters within pJIA, each exhibiting varying inflammation levels.ConclusionThese findings indicate the pivotal role of immuno-inflammation driven by classical monocytes in pJIA and reveals the existence of two subclusters within pJIA, regardless the positivity of rheumatoid factor and anti-CCP, paving the way to precision medicine.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic signatures of classical monocytes reveal pro-inflammatory modules and heterogeneity in polyarticular juvenile idiopathic arthritis

Hounkpe,

Sales,

Ribeiro

et al. 2024

Front. Immunol.

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“…Human corneal fibroblast cells were cultured in a three-dimensional (3D) collagen gel which was treated by EGCG. The study showed that EGCG can inhibit the inflammatory factor IL-1β, leading to the reduction of urokinase-type plasminogen activators in corneal fibroblasts [ 76 ].…”

Section: Therapeutic Uses In Eye Diseasesmentioning

confidence: 99%

“…As a result, EGCG requires additional research as a potential therapy for corneal ulcer. [ 76 ] in-vivo rat glaucoma EGCG (50 mg/kg/day) The NF-κB signaling pathway is involved in the anti-inflammatory impact of EGCG. [ 85 ] in-vivo rat retinal ganglion cells glaucoma GTE (275 mg/kg) To summarize, under ischemic conditions, GTE provides neuroprotection to retinal ganglion cells, which proposes a prospective therapeutic approach for the treatment of glaucoma and optic neuropathies.…”

Section: Therapeutic Uses In Eye Diseasesmentioning

confidence: 99%

Potential therapeutic effects of green tea (Camellia sinensis) in eye diseases, a review

Boroughani,

Tahmasbi,

Heidari

et al. 2024

Heliyon

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“…To study corneal ulcer, proinflammatory cytokine IL-1β is able to induced corneal collagen degradation ( 109 ). In IL-1β treated three-dimensional (3D) cultured human corneal fibroblast, EGCG treatment could suppress collagen degradation in a concentration-dependent manner ( 110 ). EGCG was found to suppressed the conversion of exogenous plasminogen to plasmin induced by IL-1β, leading to the inactivation of pro-MMP1.…”

Section: Anti-inflammatory Effects Of Gte In Eye Diseasesmentioning

confidence: 99%

Anti-inflammatory Effects of GTE in Eye Diseases

et al. 2021

Front. Nutr.

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Ocular inflammation is a common complication of various eye diseases with wide consequences from irritations to potentially sight-threatening complications. Green tea is a popular beverage throughout the world. One of the proven health benefits of consuming green tea extract (GTE) is anti-inflammation. Catechins are the biologically active constituents of GTE. In in vitro and in vivo studies, GTE and catechins present inhibition of inflammatory responses in the development of ocular inflammation including infectious, non-infectious or autoimmune, and oxidative-induced complications. Research on the ocular inflammation in animal models has made significant progress in the past decades and several key disease mechanisms have been identified. Here we review the experimental investigations on the effects of GTE and catechins on various ocular inflammation related diseases including glaucoma, age-related macular degeneration, uveitis and ocular surface inflammation. We also review the pharmacokinetics of GTE constituents and safety of green tea consumption. We discuss the insights and perspectives of these experimental results, which would be useful for future development of novel therapeutics in human.

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Inhibition by Epigallocatechin Gallate of IL-1–Induced Urokinase-Type Plasminogen Activator Expression and Collagen Degradation by Corneal Fibroblasts

Abstract: Citation: Sugioka K, Yoshida K, Murakami J, et al. Inhibition by epigallocatechin gallate of IL-1-induced urokinase-type plasminogen activator expression and collagen degradation by corneal fibroblasts. Invest Ophthalmol Vis Sci. 2019;60:2895-2903.

Cited by 10 publications

References 46 publications

Transcriptomic signatures of classical monocytes reveal pro-inflammatory modules and heterogeneity in polyarticular juvenile idiopathic arthritis

Transcriptomic signatures of classical monocytes reveal pro-inflammatory modules and heterogeneity in polyarticular juvenile idiopathic arthritis

Potential therapeutic effects of green tea (Camellia sinensis) in eye diseases, a review

Anti-inflammatory Effects of GTE in Eye Diseases

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