Inhibition by gamma‐irradiation and antimicrobial food additives of aflatoxin B₁ production by Aspergillus flavus in poultry diet

Abstract

SummaryThe effect of gamma-irradiation and the antimicrobial food additives on growth and aflatoxin B, production by A . fluuus NRRL 5520 in poultry diet was investigated. By increasing the irradiation doses the viable population as well as aflatoxin B, production decreased greatly. No growth and no detection of aflatoxin production occurred in poultry diet after treatment with 6 kGy. The addition of the antimicrobial food additives resulted in a significant decrease in aflatoxin B,. Growth and toxin production was completely inhibited by 0.64% sodium propionate, 0.134% potassium sorbate and 0.1 % sodium bisulfite. The combination treatments between gamma-irradiation (3 kGy) and sodium propionate (0.48%), potassium sorbate (0.022% and 0.044%) and sodium bisulfite (0.0334% and 0.0667%) inhibited, completely the fungal growth and aflatoxin B, production. At 5 kGy, no growth was recorded and aflatoxin B, production was completely inhibited at all tested levels of priopionate, sorbate and bisulfite. The combined effects between gamma-irradiation and the antimicrobial agents had a synergistic effect on the mould growth and aflatoxin production in poultry diet.

Effect of γ‐irradiation on aflatoxin B1 production by Aspergillus flavus and chemical composition of three crop seeds

“…Species of Aspergilli, Penicilli, Fusarium, Alternaria, Cladosporium, Helminthosporium, Curvularia, Epicoccum and Verticillium were common and widespread in soil, litter as well as many food and feed ingredients [18,23,24]. A. flavus and A. parasiticus were observed as the main popular fungal species recovered from food and feed in Egypt [3,9,10,25]. only 3 isolates (37.5%) from fababean produced aflatoxin B 1 at levels of 70-100 lg N kg -1 .…”

“…Factors, such as temperature, moisture, grain type, endogenous fungal species, storage history and length of storage, are known to influence the yield of mycotoxins [3,[7][8][9]. There has been increasing interest in the use of ionizing radiation for killing endogenous and mycotoxigenic microorganisms [10][11][12].…”

Haemato-immunology and histo-architectural changes in Labeo rohita fingerlings: effect of dietary aflatoxin and mould inhibitor

“…Among the chemical compounds, complete inhibition of mould growth and biosynthesis of aflatoxin is reported at an inclusion of 0.5% of propionic acid was reported by Ghosh et al (1996). El-Far et al (1992) reported complete inhibition of A. flavus growth and aflatoxin production at 0.64% inclusion levels of sodium propionate. Although these mould inhibitors check the fungal growth but their effect on immuno-physiological parameters of fish needs to be thoroughly studied.…”

Correlation of growth and aflatoxin production by Aspergillus flavus with some essential metals in gamma irradiated crushed corn

“…Each determination was conducted in triplicate and the results were recorded as mean value. Positive samples were further confirmed by reaction with trifluoroacetic acid and by the change to yellow fluorescence with 5% H 2 SO 4 [14].…”

“…There has been increasing interest in the use of ionizing radiation, particularly high-energy electron beams for killing endogenous insects and microorganisms in stored cereal grains [5,6,11,14,15,16,18,27,31,32,35,38]. There are number of conflicting reports which suggest that the production of mycotoxins is either increased [28,34], decreased [35,36,38] or unaffected [27] after irradiation of grains or spores under various laboratory conditions.…”