Inhibition by nisin of K+ uptake in a sensitivePediococcus sp. strain

Abstract

SUMMARYA nisin-sensitive strain of Pediococcus sp possessed an uptake system for K + which was apparently dependent on metabolic energy and ATPase activity. K + uptake rate was dependent on the glucose and K + eoncentratious and showed approximately Michaelis-Menten kinetics with respect to both of these variables with K t values of 1.2 mM and 599/xM respectively.The presence of nisin inhibited K + uptake with the percentage inhibition proportional to the nisin activity,. Total inhibition occurred at between 4.5 and 5.0 IU ml ~ and the MIC was approximately 0.6 IU ml -l.

Nisin, temperature and pH effects on growth and viability of Pectinatus frisingensis, a Gram-negative, strictly anaerobic beer-spoilage bacterium

“…This latter ion is involved in cell turgor pressure maintenance, enzyme activation, and intracellular pH homeostasis regulation (Bakker 1993). Consequently, cell damage and changes in membrane permeability could be indirectly determined by potassium movements (White et al . 1992; Ray 1993; Abee et al .…”

Glucose uptake by Listeria monocytogenes Scott A and inhibition by pediocin JD

“…neutral pH maintain a proton motive force (4p) of about -120 mV (6), and energized, growing cells maintain a somewhat higher Ap (about -160 mV [4]), there are only a few reports demonstrating 4p-coupled metabolic activities in this organism (16,22). That the 4p in L. monocytogenes is dissipated by nisin (4,5), pediocins (5)(6)(7), and other bacteriocins (16) suggests that bacteriocin-mediated inhibition of Listeria spp.…”

“…Various investigators have suggested that bacteriocin-mediated deenergization of the cytoplasmic membrane was responsible for reduced rates of amino acids and potassium uptake by sensitive cells (22). Recently, Abee et al (1) showed that lactacin F, a two-component bacteriocin produced by Lactobacillus johnsonii, caused phosphate efflux, resulting in hydro-lysis of intracellular ATP and decreased growth rates in sensitive cells.…”