Inhibition by Tyroserleutide (YSL) on the Invasion and Adhesion of the Mouse Melanoma Cell

Abstract

Tyroserleutide (YSL) is an active, low-molecular-weight polypeptide, comprised of three amino acids, that has shown antitumor effects on human hepatocarcinoma BEL-7402 in vitro and in vivo. In this study, we evaluated the inhibition of YSL on invasion and adhesion of the mouse B16-F10 melanoma cell line by injecting B16-F10 cells into the tail veins of C57BL/6 mice to establish an experimental lung metastasis model. YSL inhibited B16-F10 cell metastasis to lung, reducing the number and area of metastasis lesions. When we treated B16-F10 cells with YSL (0.01, 0.1, 1, 10, or 100 μg/mL) in vitro, we found that YSL inhibited the proliferation of B16-F10 cells with a 28.11% rate of inhibition. YSL significantly decreased the adhesiveness of B16-F10 cells to Matrigel with a 29.15% inhibition rate; YSL also significantly inhibited the invasion of B16-F10 cells, producing an inhibition of 35.31%. By analyses with Western blot and real-time RT-PCR, we found that YSL markedly inhibited the expression of ICAM-1 in B16-F10 cells. These data suggest that YSL inhibits the growth, invasion, and adhesion of B16-F10 cells.

Inhibition of metastatic lung cancer in C57BL/6 mice by liposome encapsulated all trans retinoic acid (ATRA)

“…Specifically, the B16F10 line has been shown to metastasize to the lungs when injected into the lateral tail vein and the majority of cells find themselves in the pulmonary tissue, but some are also localized in other organs [39] and is accepted as useful model for the study of lung metastasis [40,41]. Several other research studies stated the establishment of the experimental lung metastasis model or pulmonary metastatic model by intravenously injected mouse melanoma B16F10 in C57BL/6 mice [35,42,43]. Once B16F10 cells form tumor nodules in the lungs, they in turn promote lung fibrosis and collagen deposition.…”

Antimetastatic activity and low systemic toxicity of tetradecyl gallate in a preclinical melanoma mouse model

“…Numerous studies have shown that the growth and progression of most solid cancers are adhesion-and angiogenesis-dependent [34,35]. It has been reported that the degree of malignancy is directly proportional to the expression of ICAM-1 and VCAM-1 [36,37]. Both molecules are associated with melanoma progression and with an increased risk of the development of metastases.…”

A Low Molecular Weight Polysaccharide Isolated from Agaricus blazei Murill (LMPAB) Exhibits Its Anti-Metastatic Effect by Down-Regulating Metalloproteinase-9 and Up-Regulating Nm23-H1

“…We preferably focused on the low molecular weight polysaccharides, based on the previous reports on their favorable properties including better solubility, spatial stability, less toxicity, easier accessibility modifications and consequently increased anti-tumor activity compared to those with high molecular weight (Bittoun et al, 1999;Kimura et al, 2006;Yao et al, 2007;Zhou et al, 2005). In the current study, we evaluated some low molecular weight derivatives isolated from AbM and identified the most potent one, LMPAB.…”