Inhibition Effects of Silver Nanoparticles against Powdery Mildews on Cucumber and Pumpkin

Abstract

Powdery mildew is one of the most devastating diseases in cucurbits. Crop yield can decline as the disease severity increases. In this study, we evaluated the effect of silver nanoparticles against powdery mildew under different cultivation conditions in vitro and in vivo . Silver nanoparticles (WA-CV-WA13B) at various concentrations were applied before and after disease outbreak in plants to determine antifungal activities. In the field tests, the application of 100 ppm silver nanoparticles showed the highest inhibition rate for both before and after the outbreak of disease on cucumbers and pumpkins. Also, the application of 100 ppm silver nanoparticles showed maximum inhibition for the growth of fungal hyphae and conidial germination in in vivo tests. Scanning electron microscope results indicated that the silver nanoparticles caused detrimental effects on both mycelial growth and conidial germination.

Chitosan and Silver Nanoparticles as Control Agents of Some Faba Bean Spot Diseases

“…ChNPs 20 ppm concentration was found less effective for inhibition of mycelial growth and spore germination. This respect with Lamsal et al [18] who reported that silver nanoparticles inhibited growth of Colletotrichum spp. With 50 ppm and 100 ppm by 84.56% and 93.5% respectively.…”

“…While disease inhibition calculated in basis of the equation: the cells [18,36,37]. Other reports proposed that the mechanism of nanoparticles is related to their penetration into the fungal cell [38][39][40].…”

“…Nanotechnology helps agricultural fields to reduce the impact of plant diseases by production of alternative pesticides by using nanoparticles with ability to control the pathogens [17]. A lot of works have been achieved on the development of methods for controlling phytopathogens using different types of nanoparticles [17][18][19][20]. In recent years, great considerable attention is paid to antifungal effects of chitosan and silver nanoparticles due to their antifungal properties.…”

Nanocentric Plant Health Management with Special Reference to Silver

“…The studies on the applicability of silver naoparticle for controlling plant diseases has been limited till date (Lamsal et al, 2011). Silver nanoparticles, which have a high surface area and high fraction of surface atoms, have high antimicrobial effect as compared to the bulk silver.…”

“…Not much is known about the effects of silver on phytopathogenic fungi as most studies have focused on bacterial and viral pathogens of animals. The field study has suggested that, the efficacy of silver is greatly influenced by application time and preventive applications of silver nanoparticles work better before fungal inoculam penetrate and colonize the plant tissue (Lamsal et al, 2011). The study on antifungal activity of silver nanoparticles suggested that nanometer-sized silver possesses different properties, which might come from morphological and structural (Changes (Nel et al, 2003).…”