Inhibition of acetyl CoA carboxylase by GTPγS

Detection of G Proteins by Affinity Probe Capillary Electrophoresis Using a Fluorescently Labeled GTP Analogue

“…G protein analysis is usually performed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), [21][22][23][24][25][26] filter binding assays, [27][28][29] or fluorescence assays. 27,[30][31][32][33][34][35][36] SDS-PAGE, while providing molecular weight information, is plagued by analysis times of several hours and a requirement for relatively large amounts (7-30 µg) of protein.…”

“…G protein analysis is usually performed by sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE), − filter binding assays, − or fluorescence assays. ,− SDS−PAGE, while providing molecular weight information, is plagued by analysis times of several hours and a requirement for relatively large amounts (7−30 μg) of protein. The technique can be tailored specifically for G proteins by the use of radiolabeled GTP to stain the gels; however, this method is limited to Ras-like G proteins, as heterotrimers lose their ability to bind GTP as a result of SDS treatment and nitrocellulose transfer .…”