Inhibition of Activator Protein 1 Activity by Berberine in Human Hepatoma Cells

Fukuda, Kazunori; Hibiya, Yuko; Mutoh, Michihiro; Koshiji, Masatoshi; Akao, Seigou; Fujiwara, Hisayoshi

doi:10.1055/s-2006-960795

Cited by 98 publications

(51 citation statements)

References 2 publications

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“…This result corroborates the previous finding that berberine directly inhibits the activity of activator protein-1, a transactivator of IL-6. 21 Cahlin et al 6 showed that omission of host IL-6, but not TNF-␣, IL-12 or IFN-␥, improved carcass weight loss in knockout mice bearing MCG 101 tumors. Thus, C. rhizoma may decrease IL-6 levels derived from both tumor cells and host cells.…”

Section: Discussionmentioning

confidence: 99%

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Iizuka

Hazama

Yoshimura

et al. 2002

Intl Journal of Cancer

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We previously showed that the natural herb Coptidis rhizoma has an anticachectic effect in nude mice bearing human esophageal cancer cells. We further investigated this phenomenon by examining the anticachectic effect of C. rhizoma in syngeneic mice bearing colon 26/clone 20 carcinoma cells, which cause IL-6 -related cachexia after cell injection. We evaluated nutritional parameters such as serum glucose level and wasting of adipose tissue and muscle in tumor-bearing and non-tumor-bearing mice treated with C. rhizoma (CR) supplement or a normal diet. IL-6 levels in those mice were quantified by ELISA and real-time RT-PCR. CR supplementation significantly attenuated weight loss in tumor-bearing mice without changing food intake or tumor growth. Furthermore, these mice maintained good nutritional status. IL-6 mRNA levels in tumors and spleens and IL-6 protein levels in tumors and sera were significantly lower in tumorbearing mice treated with CR supplement than in those treated with a normal diet. CR supplementation did not affect food intake, body weight, nutritional parameters and IL-6 levels in non-tumor-bearing mice. An in vitro study showed that C. rhizoma and its major component, berberine, inhibited IL-1-induced IL-6 mRNA expression in a dose-dependent manner in colon 26/clone 20 cells. Our results showed that C. rhizoma exerts an anticachectic effect on colon 26/clone 20 -transplanted mice and that its effect is associated with tumor IL-6 production. We also suggest that its effect might be due to berberine.

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Section: Discussionmentioning

confidence: 99%

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Iizuka

Hazama

Yoshimura

et al. 2002

Intl Journal of Cancer

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“…Our preliminary results with p65 7/7 cells (Table 2) suggest that such an approach is less likely to be toxic to normal cells. Apart from parthenolide, there are other medicinal plants including the genera Berberis, Coptis and Gravel root with antiin¯ammatory properties (Fukuda et al, 1999;Habtemariam, 1998). These plants may be an ideal source of NF-kB inhibitors and can be explored as therapeutic agents for breast cancer.…”

Section: Paclitaxel-induced G2/m Arrest and Apoptosis Is Regulated Bymentioning

confidence: 99%

Paclitaxel sensitivity of breast cancer cells with constitutively active NF-κB is enhanced by IκBα super-repressor and parthenolide

et al. 2000

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The transcription factor nuclear factor-kB (NF-kB) regulates genes important for tumor invasion, metastasis and chemoresistance. Normally, NF-kB remains sequestered in an inactive state by cytoplasmic inhibitor-of-kB (IkB) proteins. NF-kB translocates to nucleus and activates gene expression upon exposure of cells to growth factors and cytokines. We and others have shown previously that NF-kB is constitutively active in a subset of breast cancers. In this study, we show that constitutive activation of NF-kB leads to overexpression of the antiapoptotic genes c-inhibitor of apoptosis 2 (c-IAP2) and manganese superoxide dismutase (Mn-SOD) in breast cancer cells. Furthermore, expression of the antiapoptotic tumor necrosis factor receptor associated factor 1 (TRAF1) and defender-against cell death (DAD-1) is regulated by NF-kB in certain breast cancer cells. We also demonstrate that NF-kB-inducible genes protect cancer cells against paclitaxel as MDA-MB-231 breast cancer cells modi®ed to overexpress IkBa required lower concentrations of paclitaxel to arrest at the G2/M phase of the cell cycle and undergo apoptosis when compared to parental cells. The eect of NF-kB on paclitaxel-sensitivity appears to be speci®c to cancer cells because normal ®broblasts derived from embryos lacking p65 subunit of NF-kB and wild type littermate embryos were insensitive to paclitaxel-induced G2/M cell cycle arrest. Parthenolide, an active ingredient of herbal remedies such as feverfew (tanacetum parthenium), mimicked the eects of IkBa by inhibiting NF-kB DNA binding activity and Mn-SOD expression, and increasing paclitaxel-induced apoptosis of breast cancer cells. These results suggest that active ingredients of herbs with anti-in¯ammatory properties may be useful in increasing the sensitivity of cancers with constitutively active NF-kB to chemotherapeutic drugs. Oncogene (2000) 19, 4159 ± 4169.

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“…Berberine has tremendous potential to cure many physiological disorders; hence, it has been used in the Ayurvedic [6], Unani, and Chinese as well as Homeopathic medicine. Berberine has been shown to inhibit activator protein 1, a key transcription factor in inflammation and carcinogenesis, in human cell lines [7] and has been shown to possess antitumor properties and effectively inhibit cyclooxygenase-2 transcriptional activity in human colon cancer cells [8,9]. Berberine is known to inhibit DNA topoisomerase II [10].…”

Section: Introductionmentioning

confidence: 99%

Encapsulation of Berberine in Nano-Sized PLGA Synthesized by Emulsification Method

Khemani¹,

Sharon²,

Sharon³

2012

ISRN Nanotechnology

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Nanoparticles of PLGA (polylactide glycolic acid) were prepared using biodegradable poly (D, L-lactide-co-glycolide)-75 : 25, by emulsification method using PVA (Mol. Wt. 9000) or didodecyl dimethyl ammonium bromide (DMAB) as surfactant. Nanoparticles were morphologically characterized using scanning electron microscope (SEM) and particle size analyzer. The distribution of size of PLGA nanoparticles was in the range of 48-211 nm. Berberine, a yellow isoquinoline alkaloid that is used as traditional anticancer drug, was loaded on to PLGA nanoparticles by single emulsion as well as multiple emulsion solvent evaporation techniques. Particle size analysis showed an increase in berberine loaded PLGA NP size to 180-310 nm when PVA was used as a stabilizer. Whereas use of DMAB as a stabilizer led to precipitation. In vitro drug release analysis revealed that acidic pH of 5.5 was more suitable for release of berberine than pH 7.4.

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Inhibition of Activator Protein 1 Activity by Berberine in Human Hepatoma Cells

Cited by 98 publications

References 2 publications

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Anticachectic effects of the natural herb Coptidis rhizoma and berberine on mice bearing colon 26/clone 20 adenocarcinoma

Paclitaxel sensitivity of breast cancer cells with constitutively active NF-κB is enhanced by IκBα super-repressor and parthenolide

Encapsulation of Berberine in Nano-Sized PLGA Synthesized by Emulsification Method

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