Inhibition of active sodium transport by cytochalasin B in rat jejunum in vitro

Epithelial and subepithelial resistance of rat jejunum was measured in vitro by two independent methods. (i) Transepithelial AC impedance data were interpreted in terms of a simple parallel RpCp element (representing the epithelial cell layer) in series with an ohmic resistor RS (representing the subepithelial layers). (ii) In separate experiments, the tip of a microelectrode was positioned between epithelium and subepithelial layers and the respective resistances were obtained from DC-pulse voltage divider ratios between both structures. The total tissue resistance as measured in conventional Ussing-chamber experiments (49 +/- 4 Ohm X cm2, mean of both methods) was formed to 81 +/- 6% (40 +/- 3 Ohm X cm2) by subepithelial layers and to only 19 +/- 3% (9 +/- 1 Ohm X cm2) by the epithelial cell line. We conclude that rat jejunum is more conductive than assumed so far. In in vitro flux studies on intact jejunal sheets a pronounced back-diffusion of absorbed substances will lead to an underestimation of the true net transport capacity of this structure. This error averages about fivefold and will be found likewise in conventional short-circuit measurements.

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Epithelial and subepithelial contributions to transmural electrical resistance of intact rat jejunum, in vitro

Fromm

Schulzke

Hegel

1985

Pflugers Arch.

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Cytochalasin B affects the structural polarity of statocytes from cress roots (Lepidium sativum L.)

Hensel

1985

Protoplasma

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The effect of cytochalasin B (CB; 25 micrograms ml-1 in 1% dimethylsulfoxide, DMSO) upon the structural polarity of statocytes in cress roots is demonstrated. If normal, vertically grown roots are incubated in CB, the structural polarity of the statocytes is altered according to the developmental stage of the root. Statocytes from young roots (13 or 17 hours, additionally 7 hours CB) are characterized by proximal ER cisternae and a sparsely developed distal ER-complex. Statocytes from older roots (24 hours, additionally 7 hours CB) still accumulate distal ER, as in control roots, but at the proximal cell pole in the vicinity of the nucleus additional ER is found. These effects are reversed by washing out the drug in DMSO. Growth of the roots under a continuous supply of CB yields statocytes with sedimented nuclei, proximal ER and almost no distal ER. Together with quantitative data from morphometric studies, a dynamic model of the expression of inherent cell polarity in structural polarity is proposed.

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Membrane Abnormalities in Essential Hypertension:.

Bianchi¹,

Ferrari²,

Cusi³

et al. 1986

Ann NY Acad Sci

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Inhibition of active sodium transport by cytochalasin B in rat jejunum in vitro

Cited by 4 publications

References 27 publications

Epithelial and subepithelial contributions to transmural electrical resistance of intact rat jejunum, in vitro

Epithelial and subepithelial contributions to transmural electrical resistance of intact rat jejunum, in vitro

Cytochalasin B affects the structural polarity of statocytes from cress roots (Lepidium sativum L.)

Membrane Abnormalities in Essential Hypertension:.

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