Inhibition of aflatoxin B1 production of Aspergillus flavus isolated from peanut seeds using Lycium halimifolium Mill leaves fractions

Abstract

The aim of this study is to explore the impact of Lycium halimifolium Mill leaves extracts on mycotoxin B 1 production in vitro, as well as the inhibitor effect of the aflatoxin B 1 synthesis by Aspergillus flavus strain. The strain was isolated, identified (gi |146746162 | EF409803.1.) and positively tested for the production of Aflatoxin B 1 . The antimycotoxinogenesis effect was investigated using L. halimifolium Mill leaves consisting of butanol and ethyl acetate fractions. Indeed, the fractions were tested to stop the secretion of aflatoxin B 1 by A. flavus in submerged culture (YES), simultaneously. The growth of the strain was evaluated as biomass response. The antimycotoxinogenesis activity showed that the two fractions: ethyl acetate and butanol of L. halimifolium Mill leaves were able to inhibit significantly the synthesis of mycotoxin AFB 1 . Thus, the secretion inhibition percentage was calculated in comparison with the control, using HPLC-PDA technique, and estimated at 96.83% for butanolic fraction and 94.99% for ethyl acetate fraction. To verify its correlation with biomass, the growth inhibition percentage was also estimated. The results showed that the dry weight concentration under butanolic and ethyl acetate fractions was 1.6 and 2 g/l, respectively, corresponding to 65.22 and 56.53% of inhibition growth percentage.

Isolation of Lactobacillus strain from curdled milk and investigation of their antimycotoxinogen activity

“…These results corroborate those obtained by Botton et al (1990), attesting that this strain belongs to A. flavus. The dominance of the genus Aspergillus in the contaminating flora grain has also been reported in several studies (Reddy et al, 2010;Mosbah et al, 2017).…”