Inhibition of aflatoxin production by protein tyrosine phosphatase inhibitors, blasticidin A and dephostatin

Inoguchi, Hikari; Furukawa, Tomohiro; Yoshinari, Tomoya; Sakuda, Shohei

doi:10.2520/myco.69-2-6

Cited by 3 publications

(3 citation statements)

References 24 publications

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“…Furthermore, the majority of studies in this area are focused on biodegradation aspects, whereas the search for potential efficient inhibitors of mycotoxin production among natural compounds of plant or microbial origin seems to be underestimated. To date, the number of such studies is relatively low, and these are mainly focused on the AFB1 biosynthesis inhibitors (see, for example, [ 40 , 41 ]). Moreover, some compounds reported to suppress mycotoxin biosynthesis also possess antifungal activity; thus, it often remains unclear if they reduce mycotoxin content via suppression of its biosynthesis, or simply by reducing the mycelial biomass producing them.…”

Section: Discussionmentioning

confidence: 99%

Effect of Compactin on the Mycotoxin Production and Expression of Related Biosynthetic and Regulatory Genes in Toxigenic Fusarium culmorum

et al. 2022

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Zearalenone (ZEN) and deoxynivalenol (DON) are mycotoxins produced by various species of Fusarium fungi. They contaminate agricultural products and negatively influence human and animal health, thus representing a serious problem of the agricultural industry. Earlier we showed that compactin, a secondary metabolite of Penicillium citrinum, is able to completely suppress the aflatoxin B1 biosynthesis by Aspergillus flavus. Using the F. culmorum strain FC-19 able to produce DON and ZEN, we demonstrated that compactin also significantly suppressed both DON (99.3%) and ZEN (100%) biosynthesis. The possible mechanisms of this suppression were elucidated by qPCR-based analysis of expression levels of 48 biosynthetic and regulatory genes. Expression of eight of 13 TRI genes, including TRI4, TRI5, and TRI101, was completely suppressed. A significant down-regulation was revealed for the TRI10, TRI9, and TRI14 genes. TRI15 was the only up-regulated gene from the TRI cluster. In the case of the ZEN cluster, almost complete suppression was observed for PKS4, PKS13, and ZEB1 genes, and the balance between two ZEB2 isoforms was altered. Among regulatory genes, an increased expression of GPA1 and GPA2 genes encoding α- and β-subunits of a G-protein was shown, whereas eight genes were down-regulated. The obtained results suggest that the main pathway for a compactin-related inhibition of the DON and ZEN biosynthesis affects the transcription of genes involved in the G-protein-cAMP-PKA signaling pathway. The revealed gene expression data may provide a better understanding of genetic mechanisms underlying mycotoxin production and its regulation.

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Section: Discussionmentioning

confidence: 99%

Effect of Compactin on the Mycotoxin Production and Expression of Related Biosynthetic and Regulatory Genes in Toxigenic Fusarium culmorum

et al. 2022

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“… Yan et al (2004) showed that Achromobacter xylosoxidans secreted cyclic dipeptide cyclo(L-leucyl-L-prolyl) along with cyclo(D-leucyl-D-prolyl) and cyclo(L-prolyl-Lvalyl) inhibited the aflatoxin production from Aspergillus parasiticus . Inoguchi et al (2019) reported that both blasticidin A inhibited the aflatoxin biosynthesis which acts as protein tyrosine phosphatase inhibitors which in turn can perform as potential aflatoxin production inhibitors.…”

Section: Amino Acid-based Inhibitionsmentioning

confidence: 99%

Natural inhibitors: A sustainable way to combat aflatoxins

et al. 2022

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Among a few hundred mycotoxins, aflatoxins had always posed a major threat to the world. Apart from A. flavus, A. parasiticus, and A. nomius of Aspergillus genus, which are most toxin-producing strains, several fungal bodies including Fusarium, Penicillium, and Alternaria that can biosynthesis aflatoxins. Basically, there are four different types of aflatoxins (Aflatoxin B1 (AFB1), Aflatoxin B2 (AFB2), Aflatoxin G1 (AFG1), Aflatoxin G2 (AFG2)) are produced as secondary metabolites. There are certainly other types of aflatoxins found but they are the by-products of these toxins. The fungal agents generally infect the food crops during harvesting, storing, and/or transporting; making a heavy post-harvest as well as economic loss in both developed and developing countries. And while ingesting the crop products, these toxins get into the dietary system causing aflatoxicosis, liver cirrhosis, etc. Therefore, it is imperative to search for certain ways to control the spread of infections and/or production of these toxins which may also not harm the crop harvest. In this review, we are going to discuss some sustainable methods that can effectively control the spread of infection and inhibit the biosynthesis of aflatoxins.

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“…This indicated that the antimicrobial activity of BcA was related to its protein synthesis inhibitory activity. In subsequent research, the inhibitory activity of BcA against a protein serine/threonine phosphatase was found 8) . The relationship between the phosphatase and protein synthesis is now in progress.…”

Section: Aflatoxin Production Inhibitors (Fig 2) 1) Blasticidin Amentioning

confidence: 99%

Studies on mycotoxin detection and control by natural products

Yoshinari

2022

Mycotoxins

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Aflatoxins are a group of secondary metabolites produced by some Aspergillus species, including A. flavus and A. parasiticus, and are detected in many kinds of food such as corn, tree nuts, dried fruit, and spices. Because they are potent mutagens and are liver carcinogens in humans and a wide range of animal species, aflatoxin contamination in crops causes huge economic losses and may constitute a serious health threat. A practical use of compounds which can control aflatoxin production by fungi is expected to be an effective method to prevent aflatoxin contamination in agricultural products. Many kinds of aflatoxin production inhibitors have already been reported, and most of them are derived from plant constituents, microbial metabolites, or synthetic compounds. We have been searching for new aflatoxin production inhibitors among secondary metabolites produced by actinomycetes. In this review, the features of four aflatoxin production inhibitors, blasticidin A, blasticidin S, dioctatin A and polyoxins are introduced.

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Inhibition of aflatoxin production by protein tyrosine phosphatase inhibitors, blasticidin A and dephostatin

Cited by 3 publications

References 24 publications

Effect of Compactin on the Mycotoxin Production and Expression of Related Biosynthetic and Regulatory Genes in Toxigenic Fusarium culmorum

Effect of Compactin on the Mycotoxin Production and Expression of Related Biosynthetic and Regulatory Genes in Toxigenic Fusarium culmorum

Natural inhibitors: A sustainable way to combat aflatoxins

Studies on mycotoxin detection and control by natural products

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