Inhibition of Algae by Nystatin

Lampen, J. O.; Arnow, Peter

doi:10.1128/jb.82.2.247-251.1961

Cited by 33 publications

(9 citation statements)

References 12 publications

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“…Since it takes about two weeks to observe callus growing on the plating medium when using suspension cells, the concentration of polyene that is used for selection is much higher than that used in other reported experiments on fungi (12). Also, the high concentration of drug required in the selection medium could be due to the fact that the presence of the plant cell wall might somehow protect the plant cell membrane (3).…”

Section: Resultsmentioning

confidence: 96%

Sterol composition of nystatin and amphotericin B resistant tobacco calluses

Chiu

Bottino

Patterson

1980

Lipids

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The objective of this study was to select and characterize tobacco cell lines resistant to the polyene antibiotics Amphotericin B and Nystatin. Suspension culture cells of tobacco (Nicotiana tabacum L. 'Wisconsin 38') were treated with 0.0253 ethyl methane sulfonate (EMS) and suspended in melted agar medium containing 0.005 mg/ml Amphotericin B. Cells were also irradiated with UV from a germicidal lamp and plated in the melted agar plating medium. After 6-10 days gowth, the cultures were overplated with medium containing 750 u/ml Nystatin. All cultures were gown at 26 C in the dark. After three weeks, a single callus was isolated from the Amphotericin B treatment. Tiffs callus was subcultured, and would grow at concentrations of the antibiotic as high as 0.1 mg/ml (a 20-fold increase over the selection concentration). Six callus lines were isolated from the Nystatin treatment. After subculture and transfer to media containing higher concentrations of Nystatin, only two calli survived and would grow at 1250 u/uml. Gas liquid chromatographic analysis of the resistant and control callus lines revealed similar sterol content among all the lines. However, when comparing total sterols produced, all three polyene resistant calluses contained significantly more sterols than the control. The Amphotericin B resistant calli produced about three times as much sterol as the controls, and the Nystatin resistant caUi produced about 1.5 times as much sterol as the control. Both Nystatin resistant lines showed significant increases in stigmasterol. It appears that resistance of the three tobacco cell lines to these polyene antibiotics is due principally to varied amounts of overproduction of sterols rather than qualitative differences in sterols.

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Section: Resultsmentioning

confidence: 96%

Sterol composition of nystatin and amphotericin B resistant tobacco calluses

Chiu

Bottino

Patterson

1980

Lipids

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“…The presence of a sterol appears to be a prerequisite for sensitivity toward the polyene antibiotics. Thus far, growth inhibitory activities of the polyene antibiotics have been demonstrated against yeasts (6,14), algae (6,12), protozoa (2), and flatworms (8). In contrast, bacteria which contain no significant amount of sterols are insensitive to these antibiotics.…”

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confidence: 99%

Steroid Interference with Antifungal Activity of Polyene Antibiotics

Zygmunt¹,

Tavormina²

1966

Applied Microbiology

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Wide differences exist among the polyene antibiotics, nystatin, rimocidin, filipin, pimaricin, and amphotericin B, with reference to steroid interference with their antifungal activities against Candida albicans. Of the numerous steroids tested, ergosterol was the only one which effectively antagonized the antifungal activity of all five polyene antibiotics. The antifungal activities of nystatin and amphotericin B were the least subject to vitiation by the addition of steroids other than ergosterol, and those of filipin, rimocidin, and pimaricin were the most sensitive to interference. Attempts to delineate the structural requirements of steroids possessing polyene-neutralizing activity in growing cultures of C. albicans are discussed. The ultraviolet absorbance of certain antibiotic steroid combinations was also studied.

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“…The information available on the mechanism of action of the tetraene antifungal agent nystatin (Lampen et al, 1956(Lampen et al, , 1957Scholz et al, 1959) did not offer direct support for this hypothesis. The addition of nystatin to resting intact cells initiates a series of degradative changes which result in the destruction of a variety of enzymes and possibly of cellular structures.…”

mentioning

confidence: 99%