Inhibition of angiogenic initiation and disruption of newly established human vascular networks by juice from Morinda citrifolia (noni)

Abstract

noni, the juice of the fruit from the Morinda citrifolia plant, has been used for centuries as a medicinal agent. We tested the effects of noni juice in a three-dimensional fibrin clot matrix model using human placental vein and human breast tumor explants as sources for angiogenic vessel development. Noni in concentrations of 5% (vol/vol) or greater was highly effective in inhibiting the initiation of new vessel sprouts from placental vein explants, compared with initiation in control explants in media supplemented with an equivalent amount of saline. These concentrations of noni were also effective in reducing the growth rate and proliferation of newly developing capillary sprouts. When used at a concentration of 10% in growth media, noni was able to induce vessel degeneration and apoptosis in wells with established capillary networks within a few days of its application. We also found that 10% noni juice in media was an effective inhibitor of capillary initiation in explants from human breast tumors. In tumor explants which did show capillary sprouting, the vessels rapidly degenerated (2-3 days) in those exposed to media supplemented with 10% noni.

Fermented Noni Exudate-treated dendritic cells directly stimulate B lymphocyte proliferation and differentiation

“…Twenty thousand or 4x10 4 DCs were co-cultured with 2x10 5 splenocytes, B, CD4 + T, CD8 + T, or NK cells in a final volume of 100 μl RPMI-1640 culture medium in 96-well round bottom plates for 3 to 8 days. Sixteen hours before the end of culture, 1 μCi [methyl-…”

“…Two hundred thousand B cells were added into the insert, whilst 4x10 4 DCs were added either into the insert (co-culture) or into the well (culture separately). On day 3, 1 μCi [methyl-…”

“…It was found that Noni juice given as a daily intraperitoneal injection significantly increased the lifespan of mice implanted with Lewis lung carcinoma cells. This anti-tumor activity was found to be related to a polysaccharide-rich substance (Noni-ppt) (2,4). Further research demonstrated that Noni-ppt was capable of stimulating the release of cytokines, such as TNF-·, IL-1b, IL-12, IFN-Á and IL-10 from murine effector cells, but suppressing the release of IL-4 production.…”

Health Benefits of Morinda citrifolia (Noni): A Review

“…Thus Noni juice inhibit the growth and mutations of malignant cells and induce programmed cell death or apoptosis. 28 Polysaccharides Glucoronic acid, galactose, arabinose, rhamnose, glycosides and trisaccharide fatty acid ester showed immuno-stimulatory, immune-modulatory, antibacterial, antitumor and anticancer activity. 29 6-D-Glucopyranose pentaacetate is sulphated polysaccharides, which undermine the interaction between glucosaminoglycan and certain proteins.…”

“…They also found that 10% Noni juice in media was an effective inhibitor of capillary initiation in explants from human breast tumors. 28 Chemoprotective effect Evaluate the chemoprotective effect of ethanolic extract of M. citrifolia (EEMC) fruits by simultaneous administration orally at the dose of 100 and 200 mg/kg b.wt. in rats.…”