Inhibition of Aortic Intimal Hyperplasia and Vascular Smooth Muscle Proliferation and Extracellular Matrix Protein Expressions by Astragalus–Angelica Combination

Yan, Huifang; Peng, Xiwei; Xu, Hao; Zhu, Jiahuan; Deng, Chang-Qing

doi:10.1155/2018/1508637

“…AGE, the receptor for AGE (RAGE) binding, activated the expression of MMP-9 [ 19 ]. Yan et al [ 20 ] reported that Milkvetch root- Angelica combination inhibited the expression of MMP-9. In this study, yixintongmai inhibited the migration of VSMCs and the expression of MMP-9 induced by high glucose, suggesting the inhibition of MMP-9 expression may be one of the potential mechanisms of anti-atherosclerotic effects of yixintongmai.…”

Section: Discussionmentioning

confidence: 99%

Yixintongmai Inhibits Proliferation and Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Guo

¹

,

Zhao

²

,

Dong

³

2021

Evidence-Based Complementary and Alternative Medicine

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Objective. This study was designed to evaluate the effects of yixintongmai on proliferation, migration, and apoptosis of vascular smooth muscle cells (VSMCs) cultured with high glucose. Methods. VSMCs of the thoracic aorta from 5- to 8-week-old male Sprague-Dawley rats were cultured with normal (4.5 mM) or high (25 mM) glucose, respectively. The concentration of yixintongmai powder at 360 μg/ml was chosen according to pre-experimental results. Results. Yixintongmai inhibited the proliferation of VSMCs (CCK-8 assay: 0.75 ± 0.04 versus 0.98 ± 0.09 OD, P < 0.001 ; cell counting: 37533 ± 1861 versus 56009 ± 3779 cells/well, P < 0.001 ) and the expression of proliferating cell nuclear antigen (0.74 ± 0.08 fold, P < 0.001 ) as compared with high glucose (HG). Yixintongmai inhibited the migration of VSMCs (transwell assay: 146 ± 16 versus 265 ± 62 cells; P < 0.001 ), scratch wound assay (0.17 ± 0.01 fold, P < 0.001 ), and the expression of matrix metalloproteinases-9 (0.87 ± 0.03 fold, P < 0.001 ) as compared with HG. Yixintongmai decreased mitochondrial membrane potentials (0.36 ± 0.12 fold, P < 0.001 ) and promoted early (2.11 ± 0.20 fold, P < 0.01 ) and late (2.11 ± 0.28 fold, P < 0.01 ) apoptosis of VSMCs as compared with HG. Yixintongmai inhibited the expression of B-cell lymphoma 2 (0.83 ± 0.07 fold, P < 0.01 ) and stimulated the activity of cleaved-capase-3/caspase-3 (2.00 ± 0.12 fold, P < 0.05 ) as compared with HG. Yixintongmai inhibited reactive oxygen species generation (0.46 ± 0.03 fold, P < 0.01 ) and the expression of NADPH oxidase-1 (0.84 ± 0.04 fold, P < 0.001 ), nuclear factor-kappa B (NF-κB) p65 (0.71 ± 0.07 fold, P < 0.001 ), phosphorylated NF-κB p65 (0.39 ± 0.02 fold, P < 0.0001), and inhibited nuclear translocation of NF-κB p65 (0.87 ± 0.03 fold, P < 0.001 ) in VSMCs as compared with HG. Conclusions. Yixintongmai inhibits the proliferation and migration and promotes the apoptosis of VSMCs cultured with HG, which suggests the potential anti-atherosclerotic effects of this traditional Chinese medicine.

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“…e actin cytoskeleton could be affected in glomerular disease [20,21]. A growing number of papers have shown that the cytoskeleton is injured by oxidative stress and can be restored by Chinese herbs [22,23]. Moreover, Chinese herbs also have a positive effect on the podocyte cytoskeleton.…”

Section: Discussionmentioning

confidence: 99%

Wenyang Lishui Decoction Ameliorates Podocyte Injury in Membranous Nephropathy Rat and Cell Models by Regulating p53 and Bcl‐2

Huan

¹

,

Luo

²

,

Su

³

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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Wenyang Lishui decoction (WYD) has been frequently used to treat patients with membranous nephropathy (MN) in China. Our previous study in vitro showed that WYD aqueous extract could alleviate F-actin reorganization of podocytes induced by serum from idiopathic membranous nephropathy (IMN) patients. This study aims to investigate the effects and molecular mechanisms of WYD on MN. MN rat models were induced by cationic bovine serum albumin. Experimental rats were divided into four groups: normal, model, WYD, and benazepril. The normal group consisted of normal rats receiving distilled water for four weeks, while the model, WYD, and benazepril groups consisted of MN rats receiving distilled water, 16.5 g/kg/day WYD aqueous extract, and 10 mg/kg/day benazepril, respectively. Alanine aminotransferase, kidney function, albumin, and 24 h urine total protein (UTP) were measured. Hematoxylin-eosin and electron microscopy analyses were performed. Mouse podocytes were induced to develop cell models by serum from IMN patients with antibody to the M-type phospholipase A2 receptor and spleen and kidney Yang deficiency syndrome. They were divided into five groups: control, model, 2 mg/ml WYD, 4 mg/ml WYD, and 8 mg/ml WYD. CCK-8 assays, flow cytometry, qRT-PCR, and Western blot analyses were performed. In the animal experiment, side effects of WYD were not found. Also, there was no significant difference in kidney function among the groups. In addition, UTP level was significantly reduced, and kidney histological damage was restored in both WYD and benazepril groups but difference in UTP level between them was not found. In the cell experiment, apoptosis rate was increased in the model group while it was decreased by coincubation with WYD. Besides, mRNA and protein levels of p53 were decreased, and those of Bcl-2 were increased by treatment using WYD. In conclusion, WYD could reduce proteinuria and ameliorate podocyte injury by regulating the expression of p53 and Bcl-2. The study is registered in the Chinese Clinical Trial Registry (ChiCTR-OCH-14005137).

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“…AGE-the receptor for AGE (RAGE) binding activated the expression of MMP-9 (Bongarzone, et al, 2017). Yan et al (Yan, et al, 2018) reported that astragalus-angelica combination inhibited the expression of MMP-9. In this study, yixintongmai inhibited the migration of VSMCs and the expression of MMP-9 induced by high glucose, suggesting the inhibition of MMP-9 expression may be one of the potential mechanisms of antiatherosclerotic effects of yixintongmai.…”

Section: Discussionmentioning

confidence: 99%

Yixintongmai Inhibits Proliferation, Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Guo

¹

,

Zhao

²

,

Dong

³

2020

Preprint

0

View full text Add to dashboard Cite

Background: This study was designed to evaluate the effects of yixintongmai on proliferation, migration, and apoptosis of vascular smooth muscle cells (VSMCs) cultured with high glucose. Methods: VSMCs of the thoracic aorta from 5–8 weeks male Sprague-Dawley rats were cultured with normal (4.5 mM) or high (25 mM) glucose, respectively. The effects of yixintongmai on proliferation, migration, and apoptosis of VSMCs cultured with high glucose were evaluated. The concentration of yixintongmai powder at 360 μg/ml was chosen for this study according to pre-experimental results. Results: Yixintongmai inhibited the proliferation of VSMCs (CCK-8 assay: 0.75 ± 0.04 versus 0.98 ± 0.09 OD, P<0.001; cell counting: 37533 ± 1861 versus 56009 ± 3779 cells/well, P<0.001) and the expression of PCNA (0.74 ± 0.08 folds, P<0.001) as compared with high glucose. Yixintongmai inhibited the migration of VSMCs (transwell assay: 146 ± 16 versus 265 ± 62 cells; P<0.001; scratch wound assay: 2.69 ± 0.22 folds, P<0.001) and the expression of MMP-9 (0.87 ± 0.03 folds, P<0.001) as compared with high glucose. Yixintongmai promoted the apoptosis of VSMCs (0.36 ± 0.12 folds, P<0.001) and inhibited the expression of bcl-2 (0.83 ± 0.07 folds, P<0.01) as compared with high glucose. Yixintongmai inhibited ROS generation (0.58 ± 0.01 folds, P<0.001) and the expression of NF-κB (0.71 ± 0.07 folds, P<0.001) of VSMCs as compared with high glucose. Conclusions: Yixintongmai inhibits the proliferation, migration and promotes the apoptosis of VSMCs cultured with high glucose, which suggests the potential anti-atherosclerotic effects of this traditional Chinese medicine.

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Inhibition of Aortic Intimal Hyperplasia and Vascular Smooth Muscle Proliferation and Extracellular Matrix Protein Expressions by Astragalus–Angelica Combination

Cited by 11 publications

References 27 publications

Yixintongmai Inhibits Proliferation and Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Yixintongmai Inhibits Proliferation and Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

Wenyang Lishui Decoction Ameliorates Podocyte Injury in Membranous Nephropathy Rat and Cell Models by Regulating p53 and Bcl‐2

Yixintongmai Inhibits Proliferation, Migration and Promotes Apoptosis of Vascular Smooth Muscle Cells Cultured with High Glucose

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