Inhibition of Apoptotic Pathways in Cancer

Bildik, Ayşegül; Bayar, İrem

doi:10.5336/vetsci.2018-62141

Cited by 5 publications

(4 citation statements)

References 67 publications

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“…Decreased functions of caspases constitute one of the escape mechanisms from apoptosis. Caspases (CASP) are classified according to their structural features as inflammatory CASPs such as CASP 1, 4, and 5, initiator CASPs such as CASP 2, 8, 9, 10, and effector CASPs such as CASP 3, 6, 7 ( Bildik et al, 2018 ). In our study, we found that the levels of all of CASPs classes, which we expected to increase with siRNA-mediated STMN1 silencing, decreased.…”

Section: Discussionmentioning

confidence: 99%

siRNA-mediated stathmin1 silencing inhibits proliferation of prostate carcinoma cell line

AKSOY¹,

VİCDANLI²,

ÖNALAN³

et al. 2022

Turkish Journal of Biology

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Stathmin1 (STMN1) has been proposed as a possible prognostic marker and a potential therapeutic target for some cancers. We aimed to analyze the changes in autophagy, invasion, apoptosis-related genes in prostate cancer (PCa) cell line (PC-3), after small interfering RNA (siRNA)-mediated STMN1 silencing, and also the relationships of STMN1 expression, clinicopathological parameters, and survival (OS) in PCa cases. The STMN1 expressions were analyzed, immunohistochemically, in formalin-fixed paraffin-embedded 75 PCa and 15 benign prostatic hypertrophy (BPH) tissues. The correlation between the levels of expression STMN1, clinicopathological features, and OS was determined in PCa cases. The siRNA-mediated STMN1 incubated PC-3 cells were transfected and compared to negative control siRNAs. We determined mRNA levels in autophagy, invasion, and apoptosis genes with the combination of reverse transcription-polymerase chain reaction (RT-PCR) and western blotting in PC3 cell lines after STMN1 silencing. It was determined that STMN1 was overexpressed significantly in PCa cases, immunohistochemically. The overexpression of STMN1 was significantly correlated with the high-grade Gleason score, and it was associated with a worse prognosis of PCa cases according to the Kaplan–Meier survival analysis (p < 0.05). Significant silencing in STMN1 was determined (87.5%) after siRNA applications. Especially, invasion genes such as claudin 7, fibroblast growth factor 8, hypoxia-inducible factor 1 subunit alpha, hepatocyte growth factor, matrix metallopeptidase 2, 7 genes, markedly, decreased by siRNA-mediated STMN1silencing. STMN1 silencing was determined to significantly increase caspase 3 protein expression by using western blot analysis (p < 0.001). Although STMN1 silencing did not have a significant effect on the induction of apoptosis and autophagy-related genes in PCa cells, it was shown to affect apoptotic mechanisms through the caps3 protein. siRNA-mediated STMN1 silencing decreases proliferation in the PCa cell line. It is thought that STMN1 can serve as a potential therapeutic target in the advanced stage-PCa, especially.

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Section: Discussionmentioning

confidence: 99%

siRNA-mediated stathmin1 silencing inhibits proliferation of prostate carcinoma cell line

AKSOY¹,

VİCDANLI²,

ÖNALAN³

et al. 2022

Turkish Journal of Biology

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“…Furthermore, caspase 3 is a substance released as an inactive precursor in the form of procaspase 3. Activated by active caspase 8 or 9 in the environment, it becomes caspase 3 [31,32]. As determined by IHC, it is caspase 3 in the environment, but procaspase 3, whose synthesis begins with gene expression.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Branched Chain Amino Acid Supplement on Kidney Tissue of Exercising Rats

Aydeğer¹,

Eroğlu²

2021

Preprint

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One of the supplements used in with exercise programs is branched chain amino acids (BCAAs) which preferred because of their effect on novation of muscle protein synthesis. However, BCAAs increase their amount in the blood in a short time due to their properties. In this case, the result can increase the workload of the kidneys. Based on the information, this study investigated the effects of resistance exercise and BCAA supplements on kidney tissue. A total of 24 Wistar Albino male rats were divided equally into 4 groups: Control, BCAA, Exercise and Exercise+BCAA. In six-week study, resistance swimming exercise was applied to exercise groups. BCAA groups were given BCAA supplements at doses of 2.5 mg/kg before exercise. End of the study, histological, immunochemical, and RT-PCR analyses were performed. As a result of the findings obtained, it was found that use of BCAA supplements together with exercise caused tubular necrosis (p=0.002). A significant increase was found in caspase 3 IHC staining findings in BCAA and Exercise+BCAA groups compared to Control group (p=0.011; p=0.02). Also, KIM-1 expression levels were higher in Exercise group compared to all other groups (p=0.004; p=0.003; p=0.008). As a result, BCAA consumption in combination with resistant exercise caused damage to kidney tissue.

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“…Apoptosis process; It occurs with the activation of initiating caspase (2,8,9,10), terminator caspase (3,6,7), and inflammatory (1,4,5,11,12,13,14) caspase enzymes that enable apoptosis. Situations such as irreparable DNA damage in the cell, exposure to cytotoxic drugs, and an increase in reactive oxygen species cause the internal apoptosis mechanism to be activated [21,22,23]. Cytochrome C is a structure located between the membranes of healthy cell mitochondria and plays an important role in obtaining energy.…”

Section: Introductionmentioning

confidence: 99%

“…Cytochrome C is a structure located between the membranes of healthy cell mitochondria and plays an important role in obtaining energy. In the cell undergoing apoptosis, cytochrome c passes into the cytoplasm due to the decrease in the mitochondrial membrane potential [21]. The combination of the apoptosis-activating factor, cytochrome c, and caspase-9 forms apoptosome.…”

Section: Introductionmentioning

confidence: 99%

Investigation of anticancer and antimicrobial properties of fluorinated salicylaldimine’s Florlu salisilaldiminlerin antikanser ve antimikrobiyal özelliklerinin araştırılması

ÖZER,

SHEKHANY,

BOYRAZ

et al. 2023

Türk Doğa Ve Fen Dergisi

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Bioactive molecules with higher activation power are now needed instead of organic compounds that are widely used against drug resistance that threatens human health. The development of Schiff base (SB) compounds supported by the unique properties of the fluorine atom may increase the chances of curing of drug-resistant lung cancer and bacterial infections. A series of fluorinated salicylaldimine SB ligands (C1-10) were synthesized. Their anticancer effects on the A549 human lung carcinoma cell line and antibacterial effects on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were tested by MTT (3-(4,5-dimethylthi azol-2-yl)-2, 5-diphenyltetrazolium bromide) assay. CFSE (5(6)-Carboxyfluoresceindiacetate N-succinimidyl ester) method was used to determine the proliferative indexes (PI) of the compounds on A549 cells. The apoptosis-induce capabilities of the compounds were determined by active caspase-3 analysis and mitochondrial membrane potential analysis using rhodamine123. Morphological changes indicating apoptosis in the cells were determined by standard Hematoxylin and eosin, Giemsa and Papanicolaou staining protocols. Three fluorine-bearing (F2,4,5-SAL) SB ligands (C7) showed stronger cytotoxic activity than DOX on A549 cells (1.4 to 1.9 µM, respectively). The antiproliferative effects of the compounds were weaker than DOX. Mitochondrial membrane potential, active caspase analysis together with cell morphology analyses proved that cell death occurred by induction of apoptosis. No significant antibacterial effects of the compounds were found on the bacterial strains.

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Inhibition of Apoptotic Pathways in Cancer

Cited by 5 publications

References 67 publications

siRNA-mediated stathmin1 silencing inhibits proliferation of prostate carcinoma cell line

siRNA-mediated stathmin1 silencing inhibits proliferation of prostate carcinoma cell line

The Effects of Branched Chain Amino Acid Supplement on Kidney Tissue of Exercising Rats

Investigation of anticancer and antimicrobial properties of fluorinated salicylaldimine’s Florlu salisilaldiminlerin antikanser ve antimikrobiyal özelliklerinin araştırılması

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