Inhibition of Axotomy-induced Neuronal Apoptosis by Extracellular Delivery of a Bcl-XL Fusion Protein

Liu, Xiuhuai; Collier, R. John; Youle, Richard J.

doi:10.1074/jbc.m108930200

Cited by 45 publications

(43 citation statements)

References 69 publications

(79 reference statements)

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“…Penetratin [17], polylysine [18,19], polyarginine [20], Tat PTD [16,21], HSV VP22 [22][23][24], Kaposi FGF [25], Syn B1 [26], FGF-4 [27,28], nuclear localization signal (NLS) [29], and anthrax toxin derivative 254-amino acids (aa) peptide segment [30], diphtheria toxin 'R ' binding domain [31], MPG (HIV gp41/SV40 Tag NLS) [32], pep-1 [33], WR peptide [34], and exotoxin A [35] have all been used successfully in protein transductions. The first cargo transduction was achieved by using a homeodomain of Antennapedia (Antp) [36], which transduced neurons and other cells.…”

Section: Cell Penetrating Peptides (Cpps) For Protein Deliverymentioning

confidence: 99%

The taming of the cell penetrating domain of the HIV Tat: Myths and realities

Chauhan

Tikoo²,

Kapur

et al. 2007

Journal of Controlled Release

156

136

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Protein transduction with cell penetrating peptides over the past several years has been shown to be an effective way of delivering proteins in vitro and now several reports have also shown valuable in vivo applications in correcting disease states. An impressive bioinspired phenomenon of crossing biological barriers came from HIV transactivator Tat protein. Specifically, the protein transduction domain of HIV-Tat has been shown to be a potent pleiotropic peptide in protein delivery. Various approaches such as molecular modeling, arginine guanidinium head group structural strategy, multimerization of PTD sequence and phage display system have been applied for taming of the PTD. This has resulted in identification of PTD variants which are efficient in cell membrane penetration and cytoplasmic delivery. Inspite of these state of the art technologies, the dilemma of low protein transduction efficiency and target specific delivery of PTD fusion proteins remains unsolved. Moreover, some misconceptions about PTD of Tat in the literature require considerations. We have assembled critical information on secretory, plasma membrane penetration and transcellular properties of Tat and PTD using molecular analysis and available experimental evidences.

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Section: Cell Penetrating Peptides (Cpps) For Protein Deliverymentioning

confidence: 99%

The taming of the cell penetrating domain of the HIV Tat: Myths and realities

Chauhan

Tikoo²,

Kapur

et al. 2007

Journal of Controlled Release

156

136

View full text Add to dashboard Cite

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“…The first in vivo evidence that the chimera inhibited apoptosis came from animal studies in rat optic nerves [6]. Following injury and transection, the number of pyknotic cells decrease significantly in tissues treated with Lfn-BCL-X L .…”

Section: Ced For Stroke: Engineering Chimeric Proteins To Safeguard Nmentioning

confidence: 99%

Convection enhanced delivery for treating brain tumors and selected neurological disorders: symposium review

Vogelbaum

2007

J Neurooncol

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February, 2006, to review new accomplishments and identify promising avenues of research in this evolving but still novel therapy. Among the general subjects covered by a host of international experts in their respective fields were advances in CED technology, new clinical applications of the technology, advances in CED-related imaging procedures, reviews of current or proposed trials, new drugs and the status of projects moving from lab to clinical practice. Specific subjects included the design of new catheters, the development of mathematic models for planning, novel therapeutics for CED treatment of stroke, spinal cord degenerative disease and epilepsy, liposome-based agents administered via CED, ultra-sound driven CED, monitoring the in vivo effects of intratumoral paclitaxel and other topics. Each speaker's presentation has been abstracted along with relevant references.

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“…The efficient endosomal escape of lethal factor and edema factor has been used for the delivery of fusion proteins containing the protective antigen-binding moiety of lethal factor to deliver other enzymes to the cytosol. These fusion proteins have been successfully utilized for various tumor-therapy approaches [30,42,43], the delivery of reporter proteins [44] and the delivery of anti-apoptotic Bcl-x L [45].…”

Section: Endosomal Escapementioning

confidence: 99%

Diving through Membranes: Molecular Cunning to Enforce the Endosomal Escape of Antibody-Targeted Anti-Tumor Toxins

Fuchs

Bachran²,

Flavell

et al. 2013

Antibodies

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Membranes are vital barriers by which cells control the flux of molecules and energy between their exterior and interior and also between their various intracellular compartments. While numerous transport systems exist for ions and small molecules, the cytosolic uptake of larger biological molecules and in particular antibody-targeted drugs, is a big challenge. Inducing leakage of the plasma membrane is unfavorable since the target cell specificity mediated by the antibody would likely be lost in this case. After binding and internalization, the antibody drug conjugates reach the endosomes. Thus, enforcing the endosomal escape of anti-tumor toxins without affecting the integrity of other cellular membranes is of paramount importance. Different strategies have been developed in the last decades to overcome endosomal accumulation and subsequent lysosomal degradation of targeted protein-based drugs. In this review we summarize the various efforts made to establish efficient techniques to disrupt the endosomal membrane barrier including the use of molecular ferries such as cell penetrating peptides or viral membrane fusion proteins, endosomal leakage inducing molecules such as saponins or monensin and physicochemical methods as represented by photochemical internalization.

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Inhibition of Axotomy-induced Neuronal Apoptosis by Extracellular Delivery of a Bcl-XL Fusion Protein

Cited by 45 publications

References 69 publications

The taming of the cell penetrating domain of the HIV Tat: Myths and realities

The taming of the cell penetrating domain of the HIV Tat: Myths and realities

Convection enhanced delivery for treating brain tumors and selected neurological disorders: symposium review

Diving through Membranes: Molecular Cunning to Enforce the Endosomal Escape of Antibody-Targeted Anti-Tumor Toxins

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