Inhibition of Bacillus cereus spore outgrowth and multiplication by chitosan

Mellegård, Hilde; From, Cecilie; Christensen, Bjørn E.; Granum, Per Einar

doi:10.1016/j.ijfoodmicro.2011.06.013

Cited by 30 publications

(25 citation statements)

References 50 publications

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“…Ratio: expression in chitosan treated samples over that in untreated samples.bBayesian p value.cPutative function of protein as annotated in the B. cereus ATCC14579 genome sequence.dDomains detected using SMART search (http://smart.embl-heidelberg.de/) [40]. SS, signal sequence; TMS(n), transmembrane segment (n is the number of such domain); CD, conserved domain of unknown function; PPD, periplasmic domain; HTH, helix turn helix,; FtsX, FtsX like permease family; AAA, ATPase domain.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan

et al. 2011

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The antibacterial activity of the polysaccharide chitosan towards different bacterial species has been extensively documented. The response mechanisms of bacteria exposed to this biopolymer and the exact molecular mechanism of action, however, have hardly been investigated. This paper reports the transcriptome profiling using DNA microarrays of the type-strain of Bacillus cereus (ATCC 14579) exposed to subinhibitory concentrations of two water-soluble chitosan preparations with defined chemical characteristics (molecular weight and degree of acetylation (FA)). The expression of 104 genes was significantly altered upon chitosan A (weight average molecular weight (Mw) 36.0 kDa, FA = 0.01) exposure and 55 genes when treated with chitosan B (Mw 28.4 kDa, FA = 0.16). Several of these genes are involved in ion transport, especially potassium influx (BC0753-BC0756). Upregulation of a potassium transporting system coincides with previous studies showing a permeabilizing effect on bacterial cells of this polymer with subsequent loss of potassium. Quantitative PCR confirmed the upregulation of the BC0753 gene encoding the K+-transporting ATPase subunit A. A markerless gene replacement method was used to construct a mutant strain deficient of genes encoding an ATP-driven K+ transport system (Kdp) and the KdpD sensor protein. Growth of this mutant strain in potassium limiting conditions and under salt stress did not affect the growth pattern or growth yield compared to the wild-type strain. The necessity of the Kdp system for potassium acquisition in B. cereus is therefore questionable. Genes involved in the metabolism of arginine, proline and other cellular constituents, in addition to genes involved in the gluconeogenesis, were also significantly affected. BC2798 encoding a chitin binding protein was significantly downregulated due to chitosan exposure. This study provides insight into the response mechanisms of B. cereus to chitosan treatment and the significance of the Kdp system in potassium influx under challenging conditions.

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Section: Resultsmentioning

confidence: 99%

Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan

et al. 2011

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“…However, Mellegârd et al . () reported lower MIC and MBC values of chitosan (molecular weight = 56.8 kDa) against B. cereus of 0.063 ± 0.0 and 0.10 ± 0.02 mg mL −1 , respectively, and this may have been due to the different strains, a pH of 6.0 and a different molecular weight of chitosan. In addition, they defined MBC as a 99.0% reduction, not 99.9% as in this experiment.…”

Section: Resultsmentioning

confidence: 97%

Effect of chitosan on Bacillus cereus inhibition and quality of cooked rice during storage

Rachtanapun

Tantala

Klinmalai

et al. 2015

Int J of Food Sci Tech

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Summary Improper cooling of cooked rice at an inappropriate temperature or leaving cooked rice at room temperature can cause food poisoning attributed to Bacillus cereus. Natural food preservative of either squid or crab polymer chitosan solution was added to examine their antibacterial properties against Bacillus cereus in cooked rice during storage at 37 and 4 °C. Both types of chitosan could retard the growth of B. cereus and total aerobic counts in cooked rice stored at 37 °C up to 1 day. In addition, the effect of chitosans on the physical and textural properties of cooked rice during storage was studied. Both chitosans slightly increased the moisture content of cooked rice. However, chitosans had no effect on the whiteness and hardness of cooked rice during storage (P > 0.05). Therefore, both chitosans have a potential to be used as food preservative for cooked rice with no negative effects on rice quality.

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“…Asimismo, se ha investigado los efectos del quitosano, un polisacárido derivado de la quitina presente en el citoesqueleto de insectos y crustáceos, el cual puede inhibir la germinación de B. cereus en medio de cultivo [87]. El quitosano de alto peso molecular forma una capa que impide que las células de B. cereus tomen nutrientes del medio, mientras que el de bajo peso molecular, penetra las células causando daños morfológicos [88].…”

Section: Prevención Y Controlunclassified

Bacillus cereus un patógeno importante en el control microbiológico de los alimentos

Sánchez

Correa

Castañeda-Sandoval

2016

Rev. Fac. Nac. Salud Pública

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Inhibition of Bacillus cereus spore outgrowth and multiplication by chitosan

Cited by 30 publications

References 50 publications

Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan

Transcriptional Responses of Bacillus cereus towards Challenges with the Polysaccharide Chitosan

Effect of chitosan on Bacillus cereus inhibition and quality of cooked rice during storage

Bacillus cereus un patógeno importante en el control microbiológico de los alimentos

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