Inhibition of bacterial fouling by soft coral natural products

Dobretsov, Sergey; Al-Wahaibi, Aisha S.M.; Lai, Daowan; Al-Sabahi, Jamal Nasser; Claereboudt, Michel R.; Proksch, Peter; Soussi, B.

doi:10.1016/j.ibiod.2014.10.019

Cited by 32 publications

(7 citation statements)

References 34 publications

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“…For instance, 16:OH, 21:0, 20:1n-11, and EPA generally showed stronger associations with the tissue for most species. The fatty alcohol, 16:OH, is recognised as an inhibitor of bacterial fouling in some coral species ( Dobretsov et al, 2015 ), while EPA provides immune function ( Bergé & Barnathan, 2005 ), as well as photo-protection from ultraviolet radiation ( Pilkington et al, 2011 ). Furthermore, higher levels of phospholipids in the tissue compared to the skeleton for A. millepora , M. crassotuberculata, and P. damicornis may reflect the necessity of the outer tissues to cope with seasonal fluctuations in temperature, since regulation of phospholipid composition is suggested to reflect thermal tolerance in corals ( Revel et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

Conlan

Rocker

Francis

2017

PeerJ

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Lipids are involved in a host of biochemical and physiological processes in corals. Therefore, changes in lipid composition reflect changes in the ecology, nutrition, and health of corals. As such, accurate lipid extraction, quantification, and identification is critical to obtain comprehensive insight into a coral’s condition. However, discrepancies exist in sample preparation methodology globally, and it is currently unknown whether these techniques generate analogous results. This study compared the two most common sample preparation techniques for lipid analysis in corals: (1) tissue isolation by air-spraying and (2) crushing the coral in toto. Samples derived from each preparation technique were subsequently analysed to quantify lipids and their constituent classes and fatty acids in four common, scleractinian coral species representing three distinct morphotypes (Acropora millepora, Montipora crassotuberculata, Porites cylindrica, and Pocillopora damicornis). Results revealed substantial amounts of organic material, including lipids, retained in the skeletons of all species following air-spraying, causing a marked underestimation of total lipid concentration using this method. Moreover, lipid class and fatty acid compositions between the denuded skeleton and sprayed tissue were substantially different. In particular, the majority of the total triacylglycerol and total fatty acid concentrations were retained in the skeleton (55–69% and 56–64%, respectively). As such, the isolated, sprayed tissue cannot serve as a reliable proxy for lipid quantification or identification in the coral holobiont. The in toto crushing method is therefore recommended for coral sample preparation prior to lipid analysis to capture the lipid profile of the entire holobiont, permitting accurate diagnoses of coral condition.

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Section: Discussionmentioning

confidence: 99%

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

Conlan

Rocker

Francis

2017

PeerJ

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“…It is estimated that ~60% of the reefs are already daged either directly as the result of human activities or indirectly by factors derived from global climate change, such as ocean acidification and changes in temperature, salinity, or light [2–3]. In addition to their ecological significance, coral reefs constitute an invaluable economic resource, with plethora of natural products of biotechnological and pharmaceutical interest having been isolated from reef organisms, featuring anti-cancer, anti-microbial, anti-viral and anti-inflammatory properties [4–8]. Consequently, establishing in-vitro cultivation systems that will allow the identification of the factors that determine the successful settlement and development of coral planulae-larvae is of prime importance to the on-going world-wide efforts for reef conservation [9–10].…”

Section: Introductionmentioning

confidence: 99%

The effect of bacteria on planula-larvae settlement and metamorphosis in the octocoral Rhytisma fulvum fulvum

et al. 2019

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While increasing evidence supports a key role of bacteria in coral larvae settlement and development, the relative importance of environmentally-acquired versus vertically-transferred bacterial population is not clear. Here we have attempted to elucidate the role of post-brooding-acquired bacteria on the development of planula-larvae of the octocoral Rhytisma f. fulvum, in an in vitro cultivation system employing different types of filtered (FSW) and autoclaved (ASW) seawater and with the addition of native bacteria. A good development of larvae was obtained in polystyrene 6-well cell culture plates in the absence of natural reef substrata, achieving a 60–80% of larvae entering metamorphosis after 32 days, even in bacteria-free seawater, indicating that the bacteria acquired during the brooding period are sufficient to support planulae development. No significant difference in planulae attachment and development was observed when using 0.45 μm or 0.22 μm FSW, although autoclaving the 0.45 μm FSW negatively affected larval development, indicating the presence of beneficial bacteria. Autoclaving the different FSW homogenized the development of the larvae among the different treatments. The addition of bacterial strains isolated from the different FSW did not cause any significant effect on planulae development, although some specific strains of the genus Alteromonas seem to be beneficial for larvae development. Light was beneficial for planulae development after day 20, although no Symbiodinium cells could be observed, indicating either that light acts as a positive cue for larval development or the presence of beneficial phototrophic bacteria in the coral microbiome. The feasibility of obtaining advanced metamorphosed larvae in sterilized water provides an invaluable tool for studying the physiological role of the bacterial symbionts in the coral holobiont and the specificity of bacteria-coral interactions.

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“…Biofouling process takes place when any natural or artificial surfaces submerged into the marine environment and colonized by micro-and macroorganisms (Clare, 1996), which in turn causes major ecological and economic impacts, especially when it occurs on ship hulls or aquaculture facilities (Yebra et al, 2004;Piola et al, 2009). Since several commercial biocides such as TBT based antifouling paints have been recently banned, the screening for alternative ecofriendly antifoulants appears to be urgent (Dobretsov et al, 2015). Seaweeds could be an important source, since they produce metabolites such as amides, fatty acids, terpenoids, pyrroles, lactones and steroids with antifouling potential.…”

Section: Introductionmentioning

confidence: 99%

GC/MS identification and applications of bioactive seaweed extracts from Mediterranean coast of Egypt

Hassan

Shobier

2018

Egypt. J. of Aquatic Biolo. and Fish.

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The antibacterial activity of different seaweed species against five fish pathogens (A. hydrophila, V. parahaemolyticus, V. alginolyticus, V. damsela and Vibrio sp.) has been evaluated. The ethanolic extract of C. sinuosa (CSE1) and dichloromethane extract of C. officinalis (COM) exhibited the highest antibacterial activity with activity index (AI) = 1.4 ±0.74 and 1.2 ±0.71, respectively. When the extracts were encapsulated into calcium alginate beads their antibacterial activity against the most susceptible bacterial pathogens showed that the significant growth inhibition was against V. parahaemolyticus after 5 min post addition of the beads recording 1.4 and 2 fold decrease in the growth, respectively. Recycling of the (CA/COM) beads was carried out for 7 successive cycles with success in elimination of V. parahaemolyticus. The potential applications of encapsulated extracts for elimination of microbial load in fish rearing water and antifouling activity were proved in the current investigation. The chemical composition of the bioactive extracts (CSE1) and (COM) was determined using GC/MS analysis which revealed the presence of several constituents that have been reported to exhibit antimicrobial activity. Additionally, the dichloromethane extract (COM) produced six major components including n-nonadecane, 1,2,3propanetricarboxylic acid, 2-(acetyloxy)-, tributyl ester, 2-methylhexadecan-1-ol, 1-docosene, 1-eicosanol and chloroacetic acid, octadecyl ester. However, the ethanolic extract (CSE1) was characterized by the presence of fatty acids, fatty acids ethyl esters and aromatic hydrocarbon where the most abundant compound was n-tridecanoic acid ethyl ester.

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Inhibition of bacterial fouling by soft coral natural products

Cited by 32 publications

References 34 publications

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

A comparison of two common sample preparation techniques for lipid and fatty acid analysis in three different coral morphotypes reveals quantitative and qualitative differences

The effect of bacteria on planula-larvae settlement and metamorphosis in the octocoral Rhytisma fulvum fulvum

GC/MS identification and applications of bioactive seaweed extracts from Mediterranean coast of Egypt

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