Inhibition of basal-like breast cancer growth by FTY720 in combination with epidermal growth factor receptor kinase blockade

Martin, Janet L.; Julovi, Sohel M.; Lin, Mike Z.; Silva, Hasanthi C. de; Boyle, Frances M.; Baxter, Robert C.

doi:10.1186/s13058-017-0882-x

Cited by 29 publications

(42 citation statements)

References 49 publications

(76 reference statements)

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“…The murine triple‐negative breast cancer (TNBC) cell line 4T1 was obtained from the American Type Culture Collection (ATCC). Cell was cultured in RPMI 1640 medium supplemented with 10% foetal bovine serum (FBS; Invitrogen) and 1% penicillin/streptomycin (Hyclone, USA) at 37°C in a humidified incubator with 5% CO 2 .…”

Section: Methodsmentioning

confidence: 99%

TLR5 is a new reporter for triple‐negative breast cancer indicated by radioimmunoimaging and fluorescent staining

Shi

Liu

Zhao

et al. 2019

J Cellular Molecular Medi

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Triple‐negative breast cancer (TNBC) is a highly aggressive tumour that lacks marker for targeted diagnosis. Recently, it was reported that toll‐like receptor 5 (TLR5) was associated with some kind of tumours, especially in TNBC, but whether it could be used as a non‐invasive monitoring target is not fully understood. Here, we established TLR5− 4T1 cell line with lentivirus‐shRNA‐TLR5 knock‐down transfection (with tag GFP, green fluorescent protein, TLR5− 4T1) and control TLR5+ 4T1 cell line with negative control lentivirus transfection. The effect of TLR5 down‐regulation was detected with qPCR and Western blot. 125I‐anti‐TLR5 mAb and control isotype 125I‐IgG were prepared and injected to TLR5+/− 4T1‐bearing mice models, respectively. Whole‐body phosphor‐autoradiography, fluorescence imaging and biodistribution were performed. Furthermore, ex vivo tumour TLR5 expression was proved through immunohistochemistry staining. We found that 125I‐anti‐TLR5 mAb could bind to TLR5+ 4T1 with high affinity and specificity. Whole‐body phosphor‐autoradiography after 125I‐anti‐TLR5 mAb injection showed TLR5+ 4T1 tumour images in 24 hours, more clearly in 48 hours. Radioactivities in tumour tissues were positively related with TLR5 expression. Biodistribution assay showed that 125I‐anti‐TLR5 mAb was mainly metabolized through the liver and kidney, and 125I‐anti‐TLR5 mAb was much more accumulated in TLR5+ 4T1 tumour than TLR5− 4T1. In vivo fluorescence imaging successfully showed tumour tissues clearly both in TLR5+ and TLR5− 4T1 mice compared with lentivirus untreated 4T1 tumour. Immunohistochemistry staining showed that TLR5 expression in tumours was indeed down‐regulated in TLR5− 4T1 mice. Our results indicated that 125I‐antiTLR5 mAb was an ideal agent for non‐invasive imaging of TLR5+ tumours; TLR5 may be as a novel molecular target for TNBC non‐invasive diagnosis.

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Section: Methodsmentioning

confidence: 99%

TLR5 is a new reporter for triple‐negative breast cancer indicated by radioimmunoimaging and fluorescent staining

Shi

Liu

Zhao

et al. 2019

J Cellular Molecular Medi

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“…The conclusions for min-min type representation extension and feedback extension are presented herewith (Datta 2018;Jog 2018;Martin et al 2017;Shaffrey et al 2017 The proof is omitted since it is the same as that of Theorem 6.1.…”

Section: Four Types Of Representation Extensions and Feedback Extensimentioning

confidence: 99%

R-Fuzzy Sets in Factor Space

Liu¹,

Mi²,

Choy³

2019

JSM

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Interval-valued fuzzy sets, both-branch fuzzy sets and-Fuzzy sets are three extended forms of ordinary fuzzy sets. Compared to traditional fuzzy sets, interval-valued fuzzy sets have a stronger ability to express uncertainty because they provide more choices for the descriptions of attributes. Using both-branch fuzzy sets has solved some problems in engineering decision and engineering control. For-Fuzzy sets, a new fuzzy system is constructed, with both-branch fuzzy sets as a special case. The resulting theorems improve the flexibility of uncertainty system models and expand the range of fuzzy system applications as well.

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“…Therefore, in this study two cell lines were chosen: the metastatic 4T1 and nonmetastatic 67NR lines. The 4T1 murine mammary adenocarcinoma cells are a common model of basal-like TNBC and were used in our previous studies (19,20). For the nonmetastatic cell line, 67NR was chosen since it is a paired sister cell line to the 4T1 cells (i.e.…”

Section: Introductionmentioning

confidence: 99%

Understanding the Role of Elastic Modulus in the Relationship Between Second Harmonic Generation Scattering and Tumor Cell Motility

Elias

Desa

Nguyen

et al. 2020

Preprint

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Background Second harmonic generation (SHG) is an intrinsic optical property of fibrillar collagen. SHG directionality is quantified by F/B, the ratio of forward- to backward-propagating signal, which is affected by collagen fiber internal structure, specifically the diameter, spacing, and disorder of fibrils within a collagen fiber. We have previously shown that F/B of primary invasive ductal carcinoma sections is prognostic of metastatic outcome. One possible cause of this relationship was revealed by our observation that tumor cells’ motility on collagen I gels varied when collagen fiber internal structure in those gels was manipulated. The mechanism by which tumor cells sense changes in collagen fiber internal structure remains unknown: here we evaluate the role of elastic modulus in the relationship between collagen fiber internal structure (as reported by F/B) and tumor cell motility. Methods The 4T1 murine mammary adenocarcinoma, a model of metastatic triple negative breast cancer, (TNBC) and the 67NR line, a non-metastatic luminal phenotype, were introduced to a series of collagen-polyacrylamide mixed gels wherein collagen fiber internal structure and gel elastic modulus were independently controlled. F/B was measured with SHG, while elastic modulus was measured globally via rheometry and locally via atomic force microscopy (AFM). Tumor cell motility was quantified over three hours. Results The motility of both cell lines varied with F/B while elastic modulus was held constant, and did so at two physiological modulus values. Interestingly, 4T1 cell motility increased as F/B increased, while 67NR cell motility decreased. Conclusions Our results suggest that elastic modulus does not play a significant role in the observed relationship between collagen fiber internal structure (as reported by F/B) and tumor cell motility, for two cell lines that are models of TNBC and luminal-like breast cancer. Our observation that the two lines exhibit opposite motility trends as F/B increases is consistent with the trends in metastatic outcome versus F/B observed in our published clinical data for luminal versus basal cohorts, and suggests that a tumor’s subtype may play a role in their response to collagen fiber internal structure.

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Inhibition of basal-like breast cancer growth by FTY720 in combination with epidermal growth factor receptor kinase blockade

Cited by 29 publications

References 49 publications

TLR5 is a new reporter for triple‐negative breast cancer indicated by radioimmunoimaging and fluorescent staining

TLR5 is a new reporter for triple‐negative breast cancer indicated by radioimmunoimaging and fluorescent staining

R-Fuzzy Sets in Factor Space

Understanding the Role of Elastic Modulus in the Relationship Between Second Harmonic Generation Scattering and Tumor Cell Motility

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