Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm

Chakraborty, Poulomi; Dastidar, Debabrata Ghosh; Paul, Payel; Dutta, Sutirtha; Basu, Deeparati; Sharma, Senjuti Roy; Basu, Shreosi; Sarker, Ranojit Kumar; Sen, Asim K.; Sarkar, Amit; Tribedi, Prosun

doi:10.1007/s00203-019-01775-0

Cited by 57 publications

(36 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Moreover, production of rhamnolipids by P. aeruginosa that colonizes intubated patients was associated with the development of ventilator-associated pneumonia [29]. Other alkaloids were reported for their antibiofilm effects: caffeine [30] and 7-fluoro indole, a synthetic indole-derivative [31]. Both compounds significantly inhibited the biofilm development of P. aeruginosa and interfered with the QS by targeting swarming, motility, and several virulence factors.…”

Section: Alkaloids and Nitrogen-containing Compoundsmentioning

confidence: 99%

“…Norspermidine significantly inhibited the transcription level of lasR/I, rhlR/I, and mvfR, and modulated the QS-related virulence factors (pyocyanin, elastase activity, and protease) [33]. Hordenine ↓31% (1 mg/mL) ↓23% (1 mg/mL) ↓54% (0.5-1.0 mg/mL) ↓82% (0.5-1.0 mg/mL) [27] 7-fluoroindole ↓76% (1 mM) abolished swarming (1.0 mM) no effect no effect [31] Caffeine ↓~30% (80 µg/mL) ↓~50% (80 µg/mL) ↓~50% (80 µg/mL) ↓ (40-80 µg/mL) [30] Norspermidine ↓48-90% (10 mmol/L) ↓43-85% (10 mmol/L) ↓ 42.4% (4 mmol/L) [33] Allylisothio-cynate no effect (50 µg/mL) ↓65-70% (200-800 µg/mL) [34] Benzyl-isothiocyanate no effect (50 µg/mL) ↓70% (250-1000 µg/mL) [34] Phenylethyl-isothiocyanate ↓40% (500 µg/mL) ↓30-60% (60-240 µg/mL) [34] 1 Down arrow (↓) indicates decrease of tested activity with respect to the control (ctr). Tested concentrations are reported in parenthesis.…”

Section: Anti-biofilm Activity Of Natural Compounds Against Pseudomonas Aeruginosamentioning

confidence: 99%

See 1 more Smart Citation

Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms

et al. 2020

View full text Add to dashboard Cite

Bacteria are social organisms able to build complex structures, such as biofilms, that are highly organized surface-associated communities of microorganisms, encased within a self- produced extracellular matrix. Biofilm is commonly associated with many health problems since its formation increases resistance to antibiotics and antimicrobial agents, as in the case of Pseudomonas aeruginosa and Staphylococcus aureus, two human pathogens causing major concern. P. aeruginosa is responsible for severe nosocomial infections, the most frequent of which is ventilator-associated pneumonia, while S. aureus causes several problems, like skin infections, septic arthritis, and endocarditis, to name just a few. Literature data suggest that natural products from plants, bacteria, fungi, and marine organisms have proven to be effective as anti-biofilm agents, inhibiting the formation of the polymer matrix, suppressing cell adhesion and attachment, and decreasing the virulence factors’ production, thereby blocking the quorum sensing network. Here, we focus on plant derived chemicals, and provide an updated literature review on the anti-biofilm properties of terpenes, flavonoids, alkaloids, and phenolic compounds. Moreover, whenever information is available, we also report the mechanisms of action.

show abstract

Section: Alkaloids and Nitrogen-containing Compoundsmentioning

confidence: 99%

Section: Anti-biofilm Activity Of Natural Compounds Against Pseudomonas Aeruginosamentioning

confidence: 99%

Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms

et al. 2020

View full text Add to dashboard Cite

show abstract

“…(Moreira et al 2016). Multiple studies have reported that during disease progression, the causative organism developed biofilm within human hosts thereby making the treatment process more challenging (Donlan 2002;Chakraborty et al 2020). Microbial biofilm happens to be a complex association of microorganisms adhered to a biotic or abiotic surface (Tribedi et al 2015;Gupta et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Tryptophan interferes with the quorum sensing and cell surface hydrophobicity of Staphylococcus aureus: a promising approach to inhibit the biofilm development

Paul¹,

Chakraborty²,

Sarker³

et al. 2021

3 Biotech

Self Cite

View full text Add to dashboard Cite

Staphylococcus aureus, a Gram-positive bacterium has been implicated in a plethora of human infections by virtue of its biofilm-forming ability. Inhibition in microbial biofilm formation has been found to be a promising approach towards compromising microbial pathogenesis. In this regard, various natural and synthetic molecules have been explored to attenuate microbial biofilm. In this study, the role of an amino acid, L-tryptophan was examined against the biofilm-forming ability of S. aureus. The compound did not execute any antimicrobial characteristics, instead, showed strong antibiofilm activity with the highest biofilm inhibition at a concentration of 50 µg/mL. Towards understanding the underlying mechanism of the same, efforts were given to examine whether tryptophan could inhibit biofilm formation by interfering with the quorumsensing property of S. aureus. A molecular docking analysis revealed an efficient binding between the quorum-sensing protein, AgrA, and tryptophan. Moreover, the expression of the quorum-sensing gene (agrA) got significantly reduced under the influence of the test compound. These results indicated that tryptophan could interfere with the quorum-sensing property of the organism thereby inhibiting its biofilm formation. Further study revealed that tryptophan could also reduce the cell surface hydrophobicity of S. aureus by downregulating the expression of dltA. Moreover, the tested concentrations of tryptophan did not show any significant cytotoxicity. Hence, tryptophan could be recommended as a potential antibiofilm agent to manage the biofilm-associated infections caused by S. aureus.

show abstract

“…In nature microorganisms can live in either free-living form or as biofilm (Chakraborty et al 2020). Biofilm is defined as an organized array of homogenous or heterogeneous cluster of microorganisms embedded on a self-secreted matrix made of extracellular polymeric substances (EPS) (Cortes et al 2011).…”

Section: Introductionmentioning

confidence: 99%

An interesting approach for Biofilm disruption by Thermocouple device

Parthiban¹,

Tamarakar²,

Ghosh³

et al. 2021

CTBP

Self Cite

View full text Add to dashboard Cite

Inhibition of biofilm formation of Pseudomonas aeruginosa by caffeine: a potential approach for sustainable management of biofilm

Cited by 57 publications

References 28 publications

Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms

Plant Derived Natural Products against Pseudomonas aeruginosa and Staphylococcus aureus: Antibiofilm Activity and Molecular Mechanisms

Tryptophan interferes with the quorum sensing and cell surface hydrophobicity of Staphylococcus aureus: a promising approach to inhibit the biofilm development

An interesting approach for Biofilm disruption by Thermocouple device

Contact Info

Product

Resources

About