Inhibition of biofilm formation, quorum sensing and other virulence factors in <i>Pseudomonas aeruginosa</i> by polyphenols of <i>Gynura procumbens</i> leaves

Nain, Zulkar; Mansur, Fariha Jasin; Syed, Shifath Bin; Islam, Ariful; Azakami, Hiroyuki; Islam, Rezuanul; Karim, Mohammad Minnatul

doi:10.1080/07391102.2020.1870563

Cited by 14 publications

(10 citation statements)

References 80 publications

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“…Bacterial biofilm eradication by various phytochemicals was well documented by many workers. 24,43,44 Features of colony biofilms on Congo red agar plates demonstrated a decrease in EPS content with increasing doses of TH, which was also qualitatively supported by the CLSM study. Similarly, the Congo red binding assay indicated a quantitative decline in EPS content with increasing doses of TH, resembling the observation with the crystal violet biofilm assay.…”

Section: ■ Discussionsupporting

confidence: 66%

“…24,42 TH also showed the ability to eradicate mature biofilms significantly in a dose-dependent manner, corroborating earlier studies. 24,43 The inhibitory effect of TH on biofilm formation was found to be more than the mature biofilm eradicating effect, although viable cell numbers significantly declined in both cases with increasing treatment concentrations. Biofilm eradication by the selected doses of TH was also visualized under both SEM and CLSM studies.…”

Section: ■ Discussionmentioning

confidence: 99%

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Antimicrobial Activity of Trigonelline Hydrochloride Against Pseudomonas aeruginosa and Its Quorum-Sensing Regulated Molecular Mechanisms on Biofilm Formation and Virulence

Kar,

Mukherjee,

Barik

et al. 2024

ACS Infect. Dis.

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Pseudomonas aeruginosa, a vivid biofilm-producing bacterium, is considered a dreadful opportunistic pathogen, and thus, management of biofilm-associated infections due to multidrug resistant strains by traditional drugs currently is of great concern. This study was aimed to assess the impact of trigonelline hydrochloride, a pyridine alkaloid, on P. aeruginosa PAO1, in search of an alternative therapeutant. The effect of trigonelline on colony morphology and motility was studied along with its role on biofilm and expression virulence factors. Trigonelline influenced the colony structure, motility, biofilm architecture, and the production of virulence factors in a dose-dependent manner. Alterations in quorum sending (QS)-regulated gene expression after treatment and molecular docking analysis for certain regulator proteins confirmed its effect on the QS-system network by affecting Las, Rhl, and Pqs signaling pathways and as possible molecular targets. Thus, trigonelline might be considered as a potential chemical lead to manage biofilm-associated pathogenesis or to develop other analogues with enhanced pharmacokinetic actions.

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Section: ■ Discussionsupporting

confidence: 66%

Section: ■ Discussionmentioning

confidence: 99%

Antimicrobial Activity of Trigonelline Hydrochloride Against Pseudomonas aeruginosa and Its Quorum-Sensing Regulated Molecular Mechanisms on Biofilm Formation and Virulence

Kar,

Mukherjee,

Barik

et al. 2024

ACS Infect. Dis.

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“…The extract inhibited the biofilm formation of P. aeruginosa in a dosedependent manner and ranged from 17 to 38% ( Figure 5). These considerable antibiofilm properties are similar to that of polyphenol-rich extract from the bark of Salix tetrasperma and the leaves of Annona glabra and Gynura procumbens [54][55][56]. A recent study by Koely et al described the antibiofilm activities of Enydra fluctuans against P. aeruginosa.…”

Section: Biofilm Inhibition Assaymentioning

confidence: 64%

Phytochemical Profiling of Lavandula coronopifolia Poir. Aerial Parts Extract and Its Larvicidal, Antibacterial, and Antibiofilm Activity Against Pseudomonas aeruginosa

et al. 2021

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Infections associated with the emergence of multidrug resistance and mosquito-borne diseases have resulted in serious crises associated with high mortality and left behind a huge socioeconomic burden. The chemical investigation of Lavandulacoronopifolia aerial parts extract using HPLC–MS/MS led to the tentative identification of 46 compounds belonging to phenolic acids, flavonoids and their glycosides, and biflavonoids. The extract displayed larvicidal activity against Culex pipiens larvae (LC50 = 29.08 µg/mL at 72 h). It significantly inhibited cytochrome P-450 monooxygenase (CYP450), acetylcholinesterase (AChE), and carboxylesterase (CarE) enzymes with the comparable pattern to the control group, which could explain the mode of larvae toxification. The extract also inhibited the biofilm formation of Pseudomonas aeruginosa by 17–38% at different Minimum Inhibitory Concentrations (MICs) (0.5–0.125 mg/mL) while the activity was doubled when combined with ciprofloxacin (ratio = 1:1 v:v). In conclusion, the wild plant, L.coronopifolia, can be considered a promising natural source against resistant bacteria and infectious carriers.

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“…that rosmarinic acid demonstrated anti‐QS activity to compete with C4HSL standard by binding to the QS‐regulatory RhlR of P. aeruginosa , and decreased biofilm formation by inducing gene expression. Nain et al [66] . showed that myricetin interacted with QS‐regulatory RhlR of P. aeruginosa with nine hydrophobic bonds and exhibited more excellent activity than the control in a detailed study.…”

Section: Resultsmentioning

confidence: 99%

“…It was revealed in a detailed study by Corral-Lugo et al [65] that rosmarinic acid demonstrated anti-QS activity to compete with C4HSL standard by binding to the QS-regulatory RhlR of P. aeruginosa, and decreased biofilm formation by inducing gene expression. Nain et al [66] showed that myricetin interacted with QS-regulatory RhlR of P. aeruginosa with nine hydrophobic bonds and exhibited more excellent activity than the control in a detailed study. The highest anti-QS activity of AM from Antalya could be related to the higher amounts of rosmarinic acid and myricetin.…”

Section: Antimicrobial Anti-qs and Swarming Motility Inhibition Activ...mentioning

confidence: 99%

Citrus Honeys from Three Different Regions of Turkey: HPLC‐DAD Profiling and in Vitro Enzyme Inhibition, Antioxidant, Anti‐Inflammatory and Antimicrobial Properties with Chemometric Study

Tel‐Çayan,

Çiftçi,

Taş‐Küçükaydın

et al. 2023

Chemistry & Biodiversity

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The objectives of the present study are to compare the phenolic profiles and biological activities of 15 citrus honey samples from three different locations in Turkey using a chemometric approach. The HPLC‐DAD analysis was used to determine phenolic profiles. Nineteen phenolic compounds were identified. Gallic acid (107.14‐717.04 μg/g) was recorded as the predominant compound. AF (Antalya‐Finike) had the highest antioxidant activity in ABTS•+ (IC50: 18.01±0.69 mg/mL), metal chelating (IC50: 6.20±0.19 mg/mL) and CUPRAC (A0.50: 12.05±0.68 mg/mL) assays, while it revealed the best anti‐inflammatory activity against COX‐2 (17.28±0.22%) and COX‐1 (43.28±0.91%). AM (Antalya‐Manavgat) was the most active in β‐carotene‐linoleic acid (IC50: 10.05±0.19 mg/mL), anti‐urease (38.90±0.69%), anti‐quorum sensing and antimicrobial activities. AKO1 (Adana‐Kozan‐1) in DPPH• (IC50: 34.25±0.81 mg/mL) assay, AKU1 (Antalya‐Kumluca‐1) in tyrosinase inhibition activity (37.73±0.38%) assay, AKU2 (Antalya‐Kumluca‐2) in AChE (10.55±0.63%) and BChE (9.18±0.45%) inhibition activity assays showed the best activity. Chemometric tools were applied to the phenolic compositions and biological properties. PCA and HCA ensured that 15 citrus honey samples were grouped into 3 clusters. The results showed that myricetin, kaempferol, vanillin, protocatechuic acid, rosmarinic acid, rutin, vanillic acid, gallic acid, catechin and p‐hydroxyphenyl acetic acid are phenolic compounds that can be used in the classification of citrus honeys.

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Inhibition of biofilm formation, quorum sensing and other virulence factors in Pseudomonas aeruginosa by polyphenols of Gynura procumbens leaves

Cited by 14 publications

References 80 publications

Antimicrobial Activity of Trigonelline Hydrochloride Against Pseudomonas aeruginosa and Its Quorum-Sensing Regulated Molecular Mechanisms on Biofilm Formation and Virulence

Antimicrobial Activity of Trigonelline Hydrochloride Against Pseudomonas aeruginosa and Its Quorum-Sensing Regulated Molecular Mechanisms on Biofilm Formation and Virulence

Phytochemical Profiling of Lavandula coronopifolia Poir. Aerial Parts Extract and Its Larvicidal, Antibacterial, and Antibiofilm Activity Against Pseudomonas aeruginosa

Citrus Honeys from Three Different Regions of Turkey: HPLC‐DAD Profiling and in Vitro Enzyme Inhibition, Antioxidant, Anti‐Inflammatory and Antimicrobial Properties with Chemometric Study

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