Inhibition of Bombyx mori nuclear polyhedrosis virus (NPV) replication by the putative DNA helicase gene of Autographa californica NPV

Kamita, Shizuo G.; Maeda, Susumu

doi:10.1128/jvi.67.10.6239-6245.1993

Cited by 54 publications

(30 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis using the pancaspase inhibitor Z-VAD-FMK indicated that the rRNA degradation is not the result of apoptosis triggered by transiently expressed heterologous P143 proteins . These data suggest that the global translation arrest observed in B. mori cells infected with AcMNPV or HycuMNPV (Kamita & Maeda 1993;Shirata et al 1999Shirata et al , 2004) may be linked with rRNA degradation.…”

Section: Baculovirus Factor That Alters Host Range Spectrum: P143mentioning

confidence: 84%

“…In HycuMNPV-infected BmN-4 cells, HycuMNPV-encoded EP32 protein is involved in the global translation arrest induced by viral infection (Shirata et al 2010). In AcMNPV-infected BmN cells, P143 protein encoded by AcMNPV, but not BmNPV, is involved in global translation arrest (Kamita & Maeda 1993, 1996. No viral proteins that are possibly involved in global protein synthesis shutdown have been identified in TN368 cells infected with hcf-1-defective recombinant AcMNPV.…”

Section: Baculovirus Induction Of Global Protein Synthesis Shutdownmentioning

confidence: 99%

“…BmN cells induce global protein synthesis shutdown without significant suppression of viral gene transcription upon infection with AcMNPV (Kamita & Maeda 1993). Such global translation arrest does not occur in BmN cells infected with recombinant AcMNPV harboring an AcMNPV p143 gene (ac-p143) carrying a fragment of the BmNPV p143 gene (bm-p143) in place of the native ac-p143 gene, which leads to a productive infection (Kamita & Maeda 1993, 1996. Molecular analyses have demonstrated that substitution of one or two amino acid residues in Ac-P143 with the corresponding residues from Bm-P143 precludes AcMNPV-induced global translation arrest in B. mori cells and larvae, and leads to successful viral replication and a substantial yield of progeny viruses in cell lines from both S. frugiperda and B. mori (Croizier et al 1994;Argaud et al 1998).…”

Section: Baculovirus Factor That Alters Host Range Spectrum: P143mentioning

confidence: 99%

See 2 more Smart Citations

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

View full text Add to dashboard Cite

Baculoviruses, members of the family Baculoviridae, are large, enveloped viruses that contain a doublestranded circular DNA genome of 80-180 kbp, encoding 90-180 putative proteins. These viruses are exclusively pathogenic for arthropods, particularly insects, and have been developed, or are being developed, as environmentally sound pesticides and eukaryotic vectors for foreign protein expression, surface display, gene delivery for gene therapy, vaccine production and drug screening. The baculoviruses contain a set of approximately 30 core genes that are conserved among all baculovirus genomes sequenced to date. Individual baculoviruses also contain a number of lineage-or species-specific genes that have greatly impacted the diversification and evolution of baculoviruses. In this review, we first describe the general properties and biology of baculoviruses and then focus on the baculovirus genes and mechanisms involved in the replication, spread and survival of baculoviruses within the context of their diversity, evolution and insect manipulation.

show abstract

Section: Baculovirus Factor That Alters Host Range Spectrum: P143mentioning

confidence: 84%

Section: Baculovirus Induction Of Global Protein Synthesis Shutdownmentioning

confidence: 99%

Section: Baculovirus Factor That Alters Host Range Spectrum: P143mentioning

confidence: 99%

See 1 more Smart Citation

Baculoviruses: diversity, evolution and manipulation of insects

Ikeda

Hamajima

Kobayashi

2014

Entomological Science

View full text Add to dashboard Cite

show abstract

“…To determine whether inhibition of small RNA synthesis was a common feature of AcNPV infections, we examined two-dimensional gel profiles of tRNA from permissive and nonpermissive cells infected with AcNPV. BmN cells are nonpermissive for AcNPV and undergo global protein synthesis arrest during AcNPV infection (Kamita and Maeda, 1993). Labeled tRNAs from AcNPVinfected BmN cells were practically undetectable in contrast to intensely labeled tRNAs from mock-infected cells (Fig.…”

Section: Small Rna Synthesis Is Inhibited During Nonpermissive Acnpv mentioning

confidence: 91%

“…However, another factor, hcf-1 (host cell factor 1), is required for AcNPV replication in Tn368 cells and in BTI-TN5B1-4 (Hi5) cells Miller, 1995, 1996). Replacement of a small region of the AcNPV p143 helicase gene with the homologous region from the Bombyx mori NPV p143 enables AcNPV to replicate in nonpermissive BmN cells (Kamita and Maeda, 1993;Croizier et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Global Protein Synthesis Shutdown in Autographa californica Nucleopolyhedrovirus-Infected Ld652Y Cells Is Rescued by tRNA from Uninfected Cells

Mazzacano

Thiem

1999

Virology

View full text Add to dashboard Cite

Global protein synthesis arrest occurs in Autographa californica nucleopolyhedrovirus (AcNPV)-infected Ld652Y cells at late times postinfection (p.i.). A Lymantria dispar nucleopolyhedrovirus gene, hrf-1, precludes this protein synthesis arrest. We used in vitro translation assays to characterize the translation defect. Cell-free lysates prepared from uninfected Ld652Y cells, AcNPV-infected cells harvested at early times p.i., and cells infected with vAchrf-1, a recombinant AcNPV bearing hrf-1, all supported translation. Lysates prepared from AcNPV-infected Ld652Y cells at late times p.i. did not support translation, but activity was restored by adding small RNA species from mock-, vAchrf-1- (24 or 48 h p.i.), and AcNPV- (6 h p.i. ) infected cells. Small RNA species (24 and 48 h p.i.) from AcNPV-infected cells did not rescue translation. Assays of RNA species further fractionated by ion exchange chromatography demonstrated that tRNA rescued translation. Although specific defective tRNA species were not revealed by comparative two-dimensional gel analysis, analysis of (32)P-labeled tRNAs showed a reduction in de novo synthesis of small RNA isolated from AcNPV-infected cells compared with mock- and vAchrf-1-infected cells. This study suggests a mechanism of translation arrest involving defective or depleted tRNA species in AcNPV-infected Ld652Y cells.

show abstract

Deletion Analysis of Four of Eighteen Late Gene Expression Factor Gene Homologues of the Baculovirus, BmNPV

et al. 1997

View full text Add to dashboard Cite

Nucleotide sequence analysis of the genome of the baculovirus Bombyx mori nuclear polyhedrosis virus (BmNPV) identified 18 homologues of the Autographa californica NPV (AcNPV) lefs (late expression factor genes). These BmNPV lefs showed high (73-98%) amino acid sequence identities to AcNPV lefs and were localized to similar positions in the genome. One lef, p35, was previously characterized in AcNPV and BmNPV deletion experiments. Functional deletion of each of the BmNPV lef homologues was attempted here by insertion of a beta-galactosidase gene cassette into the coding region of each lef. Four of 18 BmNPV lef (39K, ie-2, lef-7, and p35) deletion mutants were successfully isolated, indicating that the other 14 BmNPV lefs were likely essential for viral replication in cell culture. Further analysis showed that deletion of lef-7, p35, and ie-2 resulted in lower levels of viral DNA replication, indicating that the BmNPV lef-7, p35, and ie-2 products have stimulating effects on DNA replication. Deletion of 39K resulted in a significantly lower level of late gene transcription and extremely low (over 10(2)-fold less at 48-80 hr p.i.) production of progeny budded virus in BmN cells. In contrast, the deletion did not affect viral DNA replication, indicating that BmNPV 39K is involved in late gene transcription. Reduced late gene expression presumably affected production and/or release of progeny budded virus particles. This was corroborated by transmission electron microscopy, which showed that virus replication was abnormal in BmN cells infected with a BmNPV mutant lacking 39K and virion production was low. Even though 39K deletion resulted in a loss of oral infectivity, the 39K deletion mutant replicated in silkworm larvae when injected into the body cavity, as did the ie-2, lef7, and p35 deletion mutants. In addition, a BmNPV homologue of the baculovirus very late expression factor gene (vif-1) found in AcNPV was essential, implying an essential function of the BmNPV vif-1 homologue at a step before the onset of very late gene expression.

show abstract

Inhibition of Bombyx mori nuclear polyhedrosis virus (NPV) replication by the putative DNA helicase gene of Autographa californica NPV

Cited by 54 publications

References 32 publications

Baculoviruses: diversity, evolution and manipulation of insects

Baculoviruses: diversity, evolution and manipulation of insects

Global Protein Synthesis Shutdown in Autographa californica Nucleopolyhedrovirus-Infected Ld652Y Cells Is Rescued by tRNA from Uninfected Cells

Deletion Analysis of Four of Eighteen Late Gene Expression Factor Gene Homologues of the Baculovirus, BmNPV

Contact Info

Product

Resources

About