Inhibition of Botrytis cinerea and accumulation of stilbene compounds by light-emitting diodes of grapevine leaves and differential expression of defense-related genes

Abstract

Grey mold (Botrytis cinerea) is one of the most common diseases to attack grapes, causing serious damage during grape production. In the present study, the effects of light-emitting diodes (LED) on the suppression of fungal growth, defense related gene expression and accumulation of stilbenic compounds were investigated. Irradiation with blue and red light inhibited lesion development relative to fluorescent light in detached leaves. Treatment of detached leaves with LED light, especially blue and red, resulted in accumulation of stilbenic compounds and differential expression of genes involved in defense response. Among five stilbenic compounds, concentrations of trans-and cispiceid were higher than those of trans-and cis-resveratrol, as well as piceatannol in both 'Campbell Early' and 'Kyoho' leaves treated with blue and red light. The gene expression of beta-1,3 glucanase (Glu), osmotin (OSM), pathogen-related protein 4a (PR4a), protease inhibitorlike protein (PILP), thaumatin-like protein (TLP), glutathione-S-transferase (GST), phenylalanine ammonialyase (PAL), chalcone synthase (CHS), and stilbene synthase (STS) were highly upregulated under blue and red LED light. The results reported here will facilitate development of alternative methods to enhance the accumulation of resveratrol compound and protect grapevine from fungal pathogen infections.

Light and Microbial Lifestyle: The Impact of Light Quality on Plant–Microbe Interactions in Horticultural Production Systems—A Review

“…On the other hand, red-and green-light-treated tomato plants have been shown to exhibit lower proline content [110]. Light of specific wavelengths (UV, blue, red) also promotes synthesis of stilbenic compounds compared with white light [46,139,140]. Stilbenes, which are low-molecular-weight phenolics, play an important role in plant defense responses by overcoming fungal pathogen attacks [110,141].…”

“…Examples of the bacterial community structure of various greenhouse-grown crops are shown in Figure 2. With respect to foliar pathogens, the focus in previous research has been on alternative control of fungi using different wavelengths of light (light qualities), rather than on bacteria [46][47][48][49][50]. However, different light technologies influence biotic and abiotic conditions in the plant environment [51].…”

An Overview of LEDs’ Effects on the Production of Bioactive Compounds and Crop Quality

“…In addition to their role in increasing in paclitaxel levels, blue LEDs also play an important role in callus growth from needles and petioles explants [ 52 ]. Moreover, it has been seen that blue and red LEDs trigger changes in the synthesis of stilbene compounds in grape plants [ 37 ]. Increase in the production of stilbene compounds is a consequence of the higher expression levels of stilbene synthase under the influence of blue and red LEDs [ 37 , 53 ].…”

Supplemental blue light increases growth and quality of greenhouse pak choi depending on cultivar and supplemental light intensity

“…The light quality has significant effects on plants' photosynthesis, growth, development, and secondary metabolites. The increasing blue light fraction could stimulate anthocyanins accumulation in lettuce leaf (Ahn et al 2015). Post-harvest supplement of blue light could enhance antioxidant capacity in some vegetables including lettuce, barley leaf, spinach and komatsuna (Cox et al 2012).…”