2009
DOI: 10.4149/av_2009_03_203
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Inhibition of Bovine herpesvirus multiplication in MDBK cells by small interfering RNAs

Abstract: Small interfering RNA (siRNA) mediated gene silencing is a promising approach in antiviral therapy. To investigate the antiviral effects of siRNAs on Bovine herpesvirus 1 (BoHV-1) multiplication, we designed and in vitro synthesized two siRNAs (siRNA-1 and siRNA-2) targeting the UL25 gene that is essential for BHV-1 multiplication. siRNA-1 and siRNA-2 inhibited the BoHV-1 multiplication in MDBK cells to a different extent, namely by 11% and 40%, respectively, as demonstrated by virus titers (TCID 50 /ml) deter… Show more

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Cited by 8 publications
(10 citation statements)
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“…Wilkes et al (2009) designed siRNAs against the glycoprotein D gene in feline herpesvirus 1 (FHV-1) and showed decrease of 84% and 77% in target viral titer (Wilkes et al, 2009). Narute et al (2009) synthesized two siRNA molecules against BHV-1-UL25 and inhibited its replication in MDBK cells. The reduction of viral titers (11% and 40%) determined in the cell culture system by TCID50 titration method (Narute et al, 2009).…”
Section: Discussionmentioning
confidence: 99%
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“…Wilkes et al (2009) designed siRNAs against the glycoprotein D gene in feline herpesvirus 1 (FHV-1) and showed decrease of 84% and 77% in target viral titer (Wilkes et al, 2009). Narute et al (2009) synthesized two siRNA molecules against BHV-1-UL25 and inhibited its replication in MDBK cells. The reduction of viral titers (11% and 40%) determined in the cell culture system by TCID50 titration method (Narute et al, 2009).…”
Section: Discussionmentioning
confidence: 99%
“…Narute et al (2009) synthesized two siRNA molecules against BHV-1-UL25 and inhibited its replication in MDBK cells. The reduction of viral titers (11% and 40%) determined in the cell culture system by TCID50 titration method (Narute et al, 2009).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…The first technology, RNAi, has clearly been shown to be highly effective in the control of viral diseases of cattle and chickens [66][67][68][69]. Despite this promise there are still considerable issues with effectively deployment of RNAi for sustained control of viral infections in livestock.…”
Section: Rna Interference (Rnai)mentioning
confidence: 97%
“…The finding concerning a knockdown of some viral genes that can inhibit virus multiplication has generated a great effort to develop siRNA as a possible antiviral agent. This novel approach has been reported to inhibit replication of viruses like foot-and-mouth disease virus, Marek΄s disease virus, avian influenza virus, infectious bronchitis virus, porcine reproductive and respiratory syndrome virus, pseudorabies virus, rabies virus, avian leucosis virus, bovine ephemeral fever virus, chicken infectious anemia virus and bovine herpes virus (Chen et al, 2004;Levy et al, 2005;Li et al, 2005;Liu et al, 2005a;Huang et al, 2006;Klupp et al, 2006;Brando et al, 2007;Chen et al, 2007;Chuang et al, 2007;Hinton and Doran, 2008;Narute et al, 2009).…”
Section: Introductionmentioning
confidence: 99%