Inhibition of brush border sucrase by polyphenols in mouse intestine

Gupta, Swati; Mahmood, Safrun; Khan, Rizwan Hasan; Mahmood, Akhtar

doi:10.1042/bsr20090012

Cited by 9 publications

(6 citation statements)

References 25 publications

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“…At 2-3 mg/mL, all the extracts (AV, SM, and TO) showed this type of behavior of significant reductions in the two peaks present in the native enzyme, which also suggest significant modifications to PL enzyme structure. The results are consistent with previous studies that have also shown interactions of polyphenols with enzymes can result in conformational changes to the tertiary structure of enzymes (68)(69)(70)(71).…”

Section: Pancreatic Lipasesupporting

confidence: 93%

Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves

2021

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Inhibition of digestive enzymes such as α-amylase and pancreatic lipase (PL) is a promising therapeutic strategy for the treatment and management of chronic health conditions such as diabetes and obesity. Therefore, the aim of this work was to determine the enzyme inhibitory activity of polyphenol-rich aqueous extracts of Amaranthus viridis (AV), Solanum macrocarpon (SM) and Telfairia occidentalis (TO) leaves, which were harvested from plants produced using multiple urea fertilizer doses (0–80 kg N/ha). Fertilizer application was applied at two time points (at planting or 2 weeks after seedling emergence). Leaf extracts were obtained using aqueous extraction (1:20, leaves:water) for 4 h at 60°C followed by centrifugation and freeze-drying of the supernatant. Results showed that the extracts inhibited α-amylase, and pancreatic lipase dose-dependently with TO extracts having significantly (p < 0.05) higher inhibitory activities for both enzymes. Fluorescence intensity and circular dichroism spectra in the presence and absence of leaf extracts indicate significant changes to the enzyme protein secondary and tertiary conformations. We conclude that the leaf extracts, especially from TO are potential agents for reducing calorie intake as a preventive or treatment tool against chronic diseases such as diabetes and obesity.

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Section: Pancreatic Lipasesupporting

confidence: 93%

Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves

2021

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“…Sucrase inhibitors such as acarbose and voglibose are already used therapeutically in DM patients. In addition, a number of compounds that have inhibitory effects on sucrase have been identified in natural materials, such as green tea [15,16,17]. In contrast, only a few studies have shown inhibitory effects of compounds on invertase [18,19,20].…”

Section: Discussionmentioning

confidence: 99%

Identification of a Novel Oligosaccharide in Maple Syrup as a Potential Alternative Saccharide for Diabetes Mellitus Patients

Sato

Nagai

Yamamoto

et al. 2019

IJMS

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The incidence of diabetes mellitus (DM) is increasing rapidly and is associated with changes in dietary habits. Although restrictions in the use of sweeteners may prevent the development of DM, this might reduce the quality of life of patients with DM. Therefore, there has been a great deal of research into alternative sweeteners. In the search for such sweeteners, we analyzed the carbohydrate content of maple syrup and identified a novel oligosaccharide composed of fructose and glucose, linked at the C-4 of glucose and the C-6 of fructose. This oligosaccharide inhibited the release of fructose from sucrose by invertase (IC50: 1.17 mmol/L) and the decomposition of maltose by α-(1-4) glucosidase (IC50: 1.72 mmol/L). In addition, when orally administered together with sucrose to rats with DM, the subsequent plasma glucose concentrations were significantly lower than if the rats had been administered sucrose alone, without having any effect on the insulin concentration. These findings suggest that this novel oligosaccharide might represent a useful alternative sweetener for inclusion in the diet of patients with DM and may also have therapeutic benefits.

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“…The quantity of maltase was maintained at 10 μL (5 mg/mL), and 15 μL of sample (0.020, 0.030 mg/ml) was measured in various concentrations of maltose (0.025–0.2 mol/L). Km and Vmax were obtained from double-reciprocal line-weaver burk plots [ 16 ]. Acarbose (10 μL, 0.002 mg/mL) and sodium phosphate buffer (15 μL, pH = 6.0) were used as positive and negative controls, respectively.…”

Section: Methodsmentioning

confidence: 99%

Altering the inhibitory kinetics and molecular conformation of maltase by Tangzhiqing (TZQ), a natural α-glucosidase inhibitor

Zhang

Wang

et al. 2020

BMC Complement Med Ther

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Background Tangzhiqing (TZQ), as a potential α-glycosidase inhibitor, possesses postprandial hypoglycaemic effects on maltose in humans. The aim of this study was to investigate the mechanisms by which TZQ attenuates postprandial glucose by interrupting the activity of maltase, including inhibitory kinetics and circular dichroism studies. Methods In this study, we determined the inhibitory effect of TZQ on maltase by kinetic analysis to determine the IC50 value and enzyme velocity studies and line weaver-burk plot generation to determine inhibition type. Acarbose was chosen as a standard control drug. After the interaction with TZQ and maltase, secondary structure analysis was conducted with a circular dichroism method. Results TZQ showed notable inhibition activity on maltase in a reversible and competitive manner with an IC50 value of 1.67 ± 0.09 μg/ml, which was weaker than that of acarbose (IC50 = 0.29 ± 0.01 μg/ml). The circular dichroism spectrum demonstrated that the binding of TZQ to maltase changed the conformation of maltase and varied with the concentration of TZQ in terms of the disappearance of β-sheets and an increase in the α-helix content of the enzyme, similar to acarbose. Conclusions This work provides useful information for the inhibitory effect of TZQ on maltase. TZQ has the potential to be an α-glycosidase inhibitor for the prevention and treatment of prediabetes or mild diabetes mellitus.

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Inhibition of brush border sucrase by polyphenols in mouse intestine

Cited by 9 publications

References 25 publications

Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves

Inhibition of the in vitro Activities of α-Amylase and Pancreatic Lipase by Aqueous Extracts of Amaranthus viridis, Solanum macrocarpon and Telfairia occidentalis Leaves

Identification of a Novel Oligosaccharide in Maple Syrup as a Potential Alternative Saccharide for Diabetes Mellitus Patients

Altering the inhibitory kinetics and molecular conformation of maltase by Tangzhiqing (TZQ), a natural α-glucosidase inhibitor

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