Inhibition of cancer cell motility and invasion by interleukin-12

Hiscox, Stephen Edward; Hallett, Maurice Bartlett; Puntis, M. C. A.; Jiang, Wen Guo

doi:10.1007/bf00121916

Cited by 37 publications

(19 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…26) IL-12 and g-linolenic acid inhibit HGF-induced tumor migration via up-regulation of cell-surface Ecadherin. 27,28) IL-4 is also shown to inhibit HGF-induced tumor migration but its mechanism is unknown. 29) In contrast, evodiamine suppressed HGF-induced tumor migration by acting to repress the cell spreading.…”

Section: -4)mentioning

confidence: 99%

Inhibition by Evodiamine of Hepatocyte Growth Factor-Induced Invasion and Migration of Tumor Cells

Ogasawara

Suzuki

2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Tumor metastasis is a major cause of death in cancer patients, and its blockade is believed to enable cancer patients to survive. 1) Thus, it is important to find promising agents with anti-metastatic activity.One key event in the formation of tumor metastasis is cell motility. A number of host-derived factors have been shown to modulate the motile activity of tumor cells. Hepatocyte growth factor (HGF) is one such factor and has been shown to stimulate the invasion and metastasis as well as migration in a variety of tumor cells.2-4) Clinical investigations also indicate the importance of HGF in tumor metastasis by the findings that a significantly elevated serum level of HGF is observed in the patients with lung, 5) prostate, 6) gastric, 7) and breast cancer. 8) From these observations, regulation of HGF activity is recognized as a possible step in tumor metastasis therapy, however, little has been reported about compounds having anti-HGF activity.We recently reported that evodiamine has remarkable inhibitory activity on in vitro invasion and migration of tumor cells.9,10) Evodiamine is one of the main constituents of Evodiae Fructus 11) and has been shown to possess anti-tumor growth, 12,13) properties. With regard to the growth inhibition by evodiamine, Fei et al. have clearly demonstrated the molecular mechanisms by which evodiamine increases the expression of the apoptosis inducer Bax and decreases the apoptosis suppressor Bcl-2 in mitochondria, triggering the activation of caspase-3 and DNA fragmentation. 13) Moreover, the existence of unknown apoptotic pathways is speculated in the evodiamine-induced apoptosis. In contrast, no reports has been seen on the effects of evodiamine on HGF activity.In the present study, we investigated the effects of evodiamine on HGF-induced invasion and migration of tumor cell lines, colon 26-L5 carcinoma, B16-F10 melanoma, and Lewis lung carcinoma. MATERIALS AND METHODS MaterialsEvodiamine and hepatocyte growth factor (HGF) were purchased from Matsuura Yakugyo Co., Ltd. (Aichi, Japan) and Funakoshi Co., Ltd. (Tokyo, Japan), respectively. Evodiamine and HGF were dissolved in dimethyl sulfoxide (DMSO) and distilled water, respectively. In each in vitro treatment, evodiamine was diluted with DMSO, and then the solution was added into cell-suspended medium to make a final concentration of 1% DMSO.Cells and Cell Culture Murine colon 26-L5 adenocarcinoma was kindly provided by Prof. I. Saiki (Toyama Med. Pharm. Univ., Inst. of Natural Medicine, Toyama, Japan). Murine B16-F10 melanoma was kindly provided by Dr. S. Wakuzawa (Hokuriku Univ., Kanazawa, Japan). Lewis lung carcinoma (LLC) was purchased from the RIKEN Cell Bank. Colon 26-L5 cells and B16-F10 cells were maintained in RPMI-1640 supplemented with 10% fetal calf serum (FCS), 2-mercaptoethanol, 100 U/ml penicillin, and 0.1 mg/ml streptomycin. LLC was maintained in DMEM with 10% FCS, 100 U/ml penicillin, and 0.1 mg/ml streptomycin.Cell Invasion and Migration Assay Tumor cell invasion through a reconstituted basement membrane ...

show abstract

Section: -4)mentioning

confidence: 99%

Inhibition by Evodiamine of Hepatocyte Growth Factor-Induced Invasion and Migration of Tumor Cells

Ogasawara

Suzuki

2004

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

show abstract

“…We and others have demonstrated that 3T3-CM, which is used as a chemoattractant in the Matrigel invasion chamber assay, contains a number of motility factors that increase the invasiveness of breast cancer cells [5,6]. Since cellular motility is known to be directed by chemotactic motility factors and secreted cytokines [2,3], the present study examined the hypothesis that 3T3-CM contains specific factors that enhance the motility of breast cancer cells.…”

Section: Discussionmentioning

confidence: 99%

“…The metastatic dissemination of tumor cells to secondary sites requires highly motile cancer cell behavior which appears to be influenced by growth factors, cytokines, and other molecules yet to be identified [2,3]. We and others have demonstrated that 3T3-CM, which is used as a chemoattractant in the Matrigel invasion chamber assay, contains a number of motility factors that increase the invasiveness of breast cancer cells [5,6].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of Antiestrogens on NIH-3T3-Fibroblast-Induced Motility of Breast Cancer Cells

Rajah

Rambo²,

Dmytryk³

et al. 2000

Chemotherapy

View full text Add to dashboard Cite

The motile behavior of tumor cells is regulated in part by growth factors, cytokines, and other endogenous factors. In some instances, stromal tissue surrounding the tumor cells produces these growth factors which interact with tumor cells and thus may play an important role in tumor proliferation and progression. We and others have shown that conditioned media from NIH 3T3 fibroblasts (3T3-CM) increases the invasiveness of breast cancer cells. The present study characterized the influence of 3T3-CM on breast cancer cell motility and examined the hypothesis that antiestrogens inhibit this 3T3-CM-induced effect. In this study we observed that 3T3-CM added to MCF-7 cells produced an immediate cell-scattering effect as determined by time-lapse videomicroscopy, scanning electron microscopy, and F-actin labeling. The results of this study indicate that keratinocyte growth factor in 3T3-CM is largely responsible for the 3T3-CM-induced scattering motility of MCF-7 cells. These results emphasize the importance of stromal-tumor cell (epithelial-mesenchymal) interactions in the motility of breast cancer cells. Further, our results demonstrate that antiestrogens (tamoxifen, ICI-182,780 and Analog II) inhibit 3T3-CM-induced motility of MCF-7 breast cancer cells. Antiestrogen treatment reduced membrane movements and the motile morphology of MCF-7 cells induced by 3T3-CM. These results suggest that antiestrogens inhibit breast cancer cell motility and that antiestrogen treatment may be used to reduce the metastatic spread of breast cancer.

show abstract

“…*: p Ͻ 0.001, as compared with control by Student's two-tailed t test. cyte growth factor (HGF)-induced motility of human colon cancer cell lines HRT 18, HT 29 and HT 115 (Hiscox et al, 1995). Isoai et al (1992) have observed that tumor invasion-inhibiting factor-2 (IIF-2) purified from bovine liver could inhibit chemotaxis of murine B16-FE7 melanoma cells to fibronectin or laminin, invasion through Matrigel and experimental lung metastasis in mice.…”

Section: Discussionmentioning

confidence: 99%

Induction of autocrine factor inhibiting cell motility from murine B16-Bl6 melanoma cells by ?-melanocyte stimulating hormone

et al. 1999

View full text Add to dashboard Cite

Inhibition of cancer cell motility and invasion by interleukin-12

Cited by 37 publications

References 38 publications

Inhibition by Evodiamine of Hepatocyte Growth Factor-Induced Invasion and Migration of Tumor Cells

Inhibition by Evodiamine of Hepatocyte Growth Factor-Induced Invasion and Migration of Tumor Cells

Influence of Antiestrogens on NIH-3T3-Fibroblast-Induced Motility of Breast Cancer Cells

Induction of autocrine factor inhibiting cell motility from murine B16-Bl6 melanoma cells by ?-melanocyte stimulating hormone

Contact Info

Product

Resources

About