Inhibition of Candida albicans yeast–hyphal transition and biofilm formation by Solidago virgaurea water extracts

Chevalier, Marlène; Medioni, Etienne; Prêcheur, Isabelle

doi:10.1099/jmm.0.041699-0

Cited by 45 publications

(40 citation statements)

References 30 publications

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“…Meanwhile, the availability of well-established pharmaceutics is greatly reduced due to the rapidly growing number of multidrugresistant strains. The current paradigm in the discovery of novel promising antimicrobial therapeutics postulates their targeting to microbial virulence factors/abilities rather than direct biocidal activity (Bink et al, 2011;Chevalier et al, 2012;Gauwerky et al, 2009;Naicker & Patel, 2013;Rajeshkumar & Sundararaman, 2012). The antifungal potential of tested SFs from plant extracts seems to fulfil these needs.…”

Section: Discussionmentioning

confidence: 99%

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

Sadowska

Budzyńska

Więckowska-Szakiel

et al. 2014

Journal of Medical Microbiology

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The antifungal activity of the saponin-rich fractions (SFs) from Medicago sativa (aerial parts and roots) and Saponaria officinalis (used as a well-known source of plant saponins) against Candida albicans reference and clinical strains, their yeast-to-hyphal conversion, adhesion, and biofilm formation was investigated. Direct fungicidal/fungistatic properties of the tested phytochemicals used alone, as well as their synergy with azoles (probably resulting from yeast cell wall instability) were demonstrated. Here, to the best of our knowledge, we report for the first time the ability of saponin-rich extracts of M. sativa and S. officinalis to inhibit C. albicans germ tube formation, limit hyphal growth, reduce yeast adherence and biofilm formation, and eradicate mature (24 h) Candida biofilm. Moreover, M. sativa SFs (mainly obtained from aerial parts), in the range of concentrations which were active modulators of Candida virulence factors, exhibited low cytotoxicity against the mouse fibroblast line L929. These properties seem to be very promising in the context of using plant-derived SFs as potential novel antifungal therapeutics supporting classic drugs or as ingredients of disinfectants. INTRODUCTIONCandida albicans is probably the best known and at the same time the most effective opportunistic fungal pathogen of humans and animals. It constitutes a commensal microflora in the gastrointestinal and genitourinary tracts of .70 % of humans. However, in certain circumstances C. albicans could be pathogenic to critically ill, immunocompromised patients or even to healthy persons. It causes a diverse range of pathologies -from local cutaneous and mucosal infections (e.g. candidiasis of nail shafts and nails, oropharyngeal candidiasis, intestinal candidiasis) to lifethreatening systemic infections, such as candidaemia, fungal pneumonia, meningitidis or endocarditis (Cannon et al., 2009;Kabir et al., 2012;Kabir & Ahmad, 2013;Sardi et al., 2013; Tlamçani & Er-rami, 2013). Endogenous candidaemia and the carrier state are the main mechanisms of Candida spp. spread and transmission, although exogenous infections are also possible (Mathé & van Dijck, 2013;Sardi et al., 2013). The pathogenicity and high invasiveness of C. albicans arise from the broad range of its virulence factors. These include tissue-damaging hydrolytic enzymes: proteases (mainly secreted aspartic proteinases), phospholipases and haemolysins, but first of all the agents/abilities contributing to its strong adherence (e.g. two main C. albicans adhesions: agglutinin-like sequence and hyphal wall protein-1), biofilm formation and the transformation of morphological forms (yeast-to-hyphal). Also, the ability of C. albicans to survive in various anatomical body sites and to evade host defences seems to be essential (Chai et al., 2009;Chandra et al., 2001;Gropp et al., 2009;Sardi et al., 2013).Invasive candidiasis usually results from tissue colonization by yeast and formation of biofilm -the structured multicellular microbial communities embedded in a self-...

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Section: Discussionmentioning

confidence: 99%

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

Sadowska

Budzyńska

Więckowska-Szakiel

et al. 2014

Journal of Medical Microbiology

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“…It has also been shown that some plant extracts efficiently inhibit the biofilm formation of C. albicans [24]. Moreover, EOs especially with known antibacterial effects have the potential to be used in food industry as preservatives and to increase the shelf life of products.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil ofMentha piperitaL.

et al. 2012

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Variations in quantity and quality of essential oil (EO) from the aerial parts of cultivated Mentha piperita were determined. The EO of air-dried sample was obtained by a hydrodistillation method and analyzed by a gas chromatography/mass spectrometry (GC/MS). The antifungal activity of the EO was investigated by broth microdilution methods as recommended by Clinical and Laboratory Standards Institute. A biofilm formation inhibition was measured by using an XTT reduction assay. Menthol (53.28%) was the major compound of the EO followed by Menthyl acetate (15.1%) and Menthofuran (11.18%). The EO exhibited strong antifungal activities against the examined fungi at concentrations ranging from 0.12 to 8.0 μL/mL. In addition, the EO inhibited the biofilm formation of Candida albicans and C. dubliniensis at concentrations up to 2 μL/mL. Considering the wide range of the antifungal activities of the examined EO, it might be potentially used in the management of fungal infections or in the extension of the shelf life of food products.

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“…However, this working dose was previously described to contain around 0·25 mg ml −1 of saponin‐like products (Chevalier et al . ). We excluded a possible saponin‐mediated detergent action by showing first that the growth of planktonic forms of the C. albicans 10231 strain and of the three different clinical isolates was not affected by the SV extract (Table ).…”

Section: Resultsmentioning

confidence: 97%

“…We previously discovered that the crude water extract from SV was very efficient to prevent formation of Candida biofilms and that it was also capable to eradicate preformed biofilms (Chevalier et al . ). This study thus revealed the potential value of this plant extract as a new candidate against oral Candida infection.…”

Section: Discussionmentioning

confidence: 97%

“…Interestingly, this inhibitory effect, that was observed with different C. albicans wild‐type oral strains, appeared specific of fungus since growth of several bacterial strains were not affected (Chevalier et al . ). In the present work, we sought to further investigate the cellular and molecular mechanisms supporting the SV extract inhibition of C. albicans biofilm.…”

Section: Introductionmentioning

confidence: 97%

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Inhibition of adhesion‐specific genes by Solidago virgaurea extract causes loss of Candida albicans biofilm integrity

et al. 2019

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Aims Candida albicans biofilms are commonly associated with severe oral infections. We previously discovered that a crude extract from the Solidago virgaurea plant (SV extract) was a potent inhibitor of C. albicans biofilm formation. Here, we further investigate the mechanisms underlying C. albicans biofilm inhibition by the SV extract. Methods and Results The SV extract was shown to inhibit laboratory and clinical C. albicans isolates adherence and hyphal transition on inert support and epithelial human cells, without affecting viability and growth of planktonic yeasts. Interestingly, RT‐PCR–based experiments demonstrated that some key genes involved in adhesion and hyphal morphological switch (e.g. Hwp1p, Ece1p, Als3p) were strongly down‐regulated by the SV extract. Moreover, antimicrobial synergy testing (checkerboard assay) demonstrated that antifungal effects of miconazole, nystatin or a common antiseptic mouthwash were synergistically improved when used in combination with the SV extract. Conclusions The SV extract prevents C. albicans biofilm formation through direct inhibition of key adherence and hyphae‐associated genes. Significance and Impact of the Study Biofilm is considered as a key virulence factor of C. albicans infection. Our discovery of an inhibitor specifically acting on genes involved in biofilm formation paves the way for the future development of a new class of antifungal product.

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Inhibition of Candida albicans yeast–hyphal transition and biofilm formation by Solidago virgaurea water extracts

Cited by 45 publications

References 30 publications

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

New pharmacological properties of Medicago sativa and Saponaria officinalis saponin-rich fractions addressed to Candida albicans

Chemical Composition, Antifungal and Antibiofilm Activities of the Essential Oil ofMentha piperitaL.

Inhibition of adhesion‐specific genes by Solidago virgaurea extract causes loss of Candida albicans biofilm integrity

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