Inhibition of Candidacidal Activity of Polymorphonuclear Cells by Alveolar Macrophage-derived Factor from Lung Cancer Patients

Vecchiarelli, Anna; Dottorini, Maurizio; Beccari, Tommaso; Cociani, Cristiana; Todisco, Tommaso; Bistoni, Francesco

doi:10.1164/ajrccm/147.2.414

Cited by 17 publications

(8 citation statements)

References 21 publications

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“…To prepare peripheral blood monocytes (PBM), heparinized venous blood obtained from normal volunteers was diluted with RPMI 1640. Mononuclear cells were isolated by Ficoll-Hypaque density gradient centrifugation (35). The cells were washed twice in RPMI, placed into cell culture petri dishes (Nunc Inter Med, Roskilde, Denmark) at a concentration of 2 ϫ 10 6 to 3 ϫ 10 6 /ml in RPMI 1640 medium supplemented with 5% FCS (cRPMI) and incubated at 37ЊC in 5% CO 2 for 1 h. The nonadherent cells were removed by washing the dishes three to five times with warm RPMI 1640 medium.…”

Section: Methodsmentioning

confidence: 99%

Downregulation by cryptococcal polysaccharide of tumor necrosis factor alpha and interleukin-1 beta secretion from human monocytes

et al. 1995

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The regulation by Cryptococcus neoformans encapsulation of interleukin 1␤ (IL-1␤) and tumor necrosis factor alpha (TNF-␣) production by human monocytes was investigated. By using encapsulated and acapsular C. neoformans, we demonstrated that both strains induce cytokine production, although the acapsular strain was a better stimulator than the thinly encapsulated strain. The cytokine levels produced by cells stimulated by the two strains were lower and followed a different kinetic than those stimulated by lipopolysaccharide (LPS). Purified capsular polysaccharide inhibits TNF-␣ secretion induced by LPS or acapsular C. neoformans. In contrast, no regulatory effect on IL-1␤ was observed when LPS was used. The secretory response of these cytokines follows different pathways of macrophage activation; in fact, complete inhibition of TNF-␣ does not affect IL-1␤ production and vice versa. These data indicate that purified capsular polysaccharide of C. neoformans could contribute to the in vivo progress of cryptococcosis by suppressing cytokine production of macrophages and suggest that a therapeutic approach to address the suppressive effect of cryptococcal polysaccharide could be devised.

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Section: Methodsmentioning

confidence: 99%

Downregulation by cryptococcal polysaccharide of tumor necrosis factor alpha and interleukin-1 beta secretion from human monocytes

et al. 1995

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“…Serum from patients with solid tumors reduces the cytostatic activity of normal PMN 34. In patients with lung cancer, a factor produced by the alveolar macrophages inhibits the candidacidal activity of PMN 380. A factor that inhibits PMN functions (phagocytosis and the production of ROS) has been detected in plasma from tumor‐bearing rats 381.…”

Section: Does Defective Pmn Function Encourage the Growth Of A Canmentioning

confidence: 99%

Polymorphonuclear neutrophils and cancer: Intense and sustained neutrophilia as a treatment against solid tumors

Souto

Vilá

Brú

2011

Medicinal Research Reviews

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Polymorphonuclear neutrophils (PMN) are the most abundant circulating immune cells and represent the first line of immune defense against infection. This review of the biomedical literature of the last 40 years shows that they also have a powerful antitumoral effect under certain circumstances. Typically, the microenvironment surrounding a solid tumor possesses many of the characteristics of chronic inflammation, a condition considered very favorable for tumor growth and spread. However, there are many circumstances that shift the chronic inflammatory state toward an acute inflammatory response around a tumor. This shift seems to convert PMN into very efficient anticancer effector cells. Clinical reports of unexpected antitumoral effects linked to the prolonged use of granulocyte colony-stimulating factor, which stimulates an intense and sustained neutrophilia, suggest that an easy way to fight solid tumors would be to encourage the development of intense peritumoral PMN infiltrates. Specifically designed clinical trials are urgently needed to evaluate the safety and efficacy of such drug-induced neutrophilia in patients with solid tumors. This antitumoral role of neutrophils may provide new avenues for the clinical treatment of cancer.

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“…Heparinized venous blood, obtained from healthy donors, was diluted with RPMI 1640. Then, mononuclear cells were separated by Ficoll-Hypaque density gradient centrifugation (32). The cells at the interface were washed twice in RPMI 1640, placed into cell culture petri dishes (Nunc Inter Med, Roskilde, Denmark) at a concentration of 2 ϫ 10 6 to 3 ϫ 10 6 /ml in RPMI 1640 supplemented with 5% fetal calf serum, and incubated at 37ЊC in 5% CO 2 for 1 h. The nonadherent cells were removed by washing the dishes three to five times with warm RPMI 1640.…”

Section: Neoformans and Cryptococcal Polysaccharidementioning

confidence: 99%

Capsular polysaccharide of Cryptococcus neoformans induces proinflammatory cytokine release by human neutrophils

et al. 1996

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Human polymorphonuclear leukocytes from normal subjects produced proinflammatory cytokines in response to stimulation with Cryptococcus neoformans yeast cells. The cytokines released after stimulation of neutrophils included interleukin-1␤ (IL-1␤), IL-6, IL-8, and tumor necrosis factor alpha. The magnitude of the cytokine response was related to the yeast capsule size. Cells of a large-capsule isolate stimulated release of greater amounts of cytokine than did a thinly encapsulated isolate, which, in turn, stimulated release of greater amounts of cytokine than an acapsular isolate. Cytokine release was also stimulated by supernatant fluids from cryptococcal cells that were preincubated with 10% human serum, suggesting the generation of a soluble mediator. The major capsular polysaccharide, glucuronoxylomannan, stimulated release of tumor necrosis factor alpha, IL-1␤, and IL-8 in a dose-dependent fashion. These results differ from previous studies of cytokine secretion by human monocytes in several important respects, including the importance of encapsulation in stimulation of cytokine secretion and the ability of purified glucuronoxylomannan to induce cytokine secretion.

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Inhibition of Candidacidal Activity of Polymorphonuclear Cells by Alveolar Macrophage-derived Factor from Lung Cancer Patients

Cited by 17 publications

References 21 publications

Downregulation by cryptococcal polysaccharide of tumor necrosis factor alpha and interleukin-1 beta secretion from human monocytes

Downregulation by cryptococcal polysaccharide of tumor necrosis factor alpha and interleukin-1 beta secretion from human monocytes

Polymorphonuclear neutrophils and cancer: Intense and sustained neutrophilia as a treatment against solid tumors

Capsular polysaccharide of Cryptococcus neoformans induces proinflammatory cytokine release by human neutrophils

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