Inhibition of carrageenin‐induced rat paw oedema by crotapotin, a polypeptide complexed with phospholipase A₂

Abstract

1 The effect of purified crotapotin, a non-toxic non-enzymatic chaperon protein normally complexed to a phospholipase A2 (PLA2) in South America rattlesnake venom, was studied in the acute inflammatory response induced by carrageenin (1 mg/paw), compound 48/80 (3 jig/paw) and 5-hydroxytryptamine (5-HT) (3 jig/paw) in the rat hind-paw. The effects of crotapotin on platelet aggregation, mast cell degranulation and eicosanoid release from guinea-pig isolated lung were also investigated.2 Subplantar co-injection of crotapotin (1 and 10 jig/paw) with carrageenin or injection of crotapotin (10 jig/paw) into the contralateral paw significantly inhibited the carrageenin-induced oedema. This inhibition was also observed when crotapotin (10-30 jig/paw) was administered either intraperitoneally or orally. Subplantar injection of heated crotapotin (15 min at 60°C) failed to inhibit carrageenininduced oedema. Subplantar injection of crotapotin (10 jig/paw) also significantly inhibited the rat paw oedema induced by compound 48/80, but it did not affect 5-HT-induced oedema. 3 In adrenalectomized animals, subplantar injection of crotapotin markedly inhibited the oedema induced by carrageenin. The inhibitory effect of crotapotin was also observed in rats depleted of histamine and 5-HT stores.4 Crotapotin (30 jig/paw) had no effect on either the histamine release induced by compound 48/80 in vitro or on the platelet aggregation induced by both arachidonic acid (1 mM) and platelet activating factor (1 jiM) in human platelet-rich plasma. The platelet aggregation and thromboxane B2 (TXB2) release induced by thrombin (100 mu ml-') in washed human platelets were also not affected by crotapotin. In addition, crotapotin (10 jig/paw) did not affect the release of 6-oxo-prostaglandin Flc, and TXB2 induced by ovalbumin in sensitized guinea-pig isolated lungs. 5 Our results indicate that the anti-inflammatory activity of crotapotin is not due to endogenous corticosteroid release or inhibition of cyclo-oxygenase activity. It is possible that crotapotin may interact with extracellular PLA2 generated during the inflammatory process thereby reducing its hydrolytic activity.

ACIDENTES CAUSADOS POR SERPENTES DO GÊNERO Crotalus EM PEQUENOS ANIMAIS – REVISÃO DE LITERATURA

“…al., 2012) e antitumoral (HERNANDEZ PLATA et al, 1993;SOARES et al, 2010;LIPPZ, 1994). Landucci et al, (1995) descreveram em seu estudo que o componente responsável pela ação antiinflamatória do veneno, é a crotapotina. Neste estudo, observou-se a redução do edema de pata de camundongos induzido por carragenina, uma substância obtida a partir de extratos de um musgo marinho da Irlanda denominado Chondrus crispus que desencadeia uma intensa resposta inflamatória caracterizada pelos altos níveis de prostaglandinas (PGs) e leucotrienos presentes no exsudato.…”

<b>Polysaccharide extract of <i>Mimosa tenuiflora</i> stem barks stimulates acute inflammatory response via nitric oxide

“…30 min prior to EP-Mt. The edema was measured by plethysmometry (Panlab LE-7500) before edema induction (zero time), at 30 min, and from 60 to 420 min thereafter, and expressed in μL or area under the curve-AUC (arbitrary units) (Landucci et al, 1995). For evaluation of plasma leakage, animals received Evans blue (25 mg kg -1 ) 30 min before edema induction by EP-Mt.…”

Edematogenic activity of a sulfated galactan from the red marine algaeGelidium crinale

“…Data were expressed as the increase in paw volume (mL) displacement along the experiment subtracted from the basal volume, measured at zero time, or area under curve (AUC; arbitrary units) (Landucci et al, 1995).…”