Inhibition of cell division during cabbage (<i>Brassica oleracea</i>L.) seed germination

Górnik, Krzysztof; Castro, Renato Delmondez de; Liu, Yongqing; Bino, R.J.; Groot, Steven P. C.

doi:10.1017/s0960258500003731

Cited by 36 publications

(33 citation statements)

References 16 publications

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“…Liu, personal communication). Similar observations were made in cabbage seeds (Gó rnik et al, 1997). This implies that cell division is not a prerequisite for radicle protrusion in tomato.…”

supporting

confidence: 84%

“…Evidently, rearrangement of microtubules and DNA synthesis are required for cell division (Gunning and Sammut, 1990;Gunning and Steer, 1996); however, there seems to be no relationship between cell expansion and DNA synthesis. First, as was shown for germination of cabbage (Brassica oleraceae L.) seeds (Gó rnik et al, 1997), DNA replication can also be inhibited in tomato by hydroxyurea without affecting cell expansion (Y. Liu and S.P.C. Groot, personal communication).…”

Section: Dna Synthesis and Appearance Of Microtubule Arrays Is Correlmentioning

confidence: 93%

“…From recent studies it is known that during imbibition of tomato seeds, DNA replication and ␤-tubulin accumulation are concentrated in the embryonic radicle tip, suggesting an intimate interplay in the preparation for radicle growth (de Castro et al, 1998). However, in cabbage it was shown that the increase in 4C DNA during imbibition could be inhibited by hydroxyurea, whereas ␤-tubulin accumulation and radicle protrusion were unaffected (Gó rnik et al, 1997). Thus, questions as to whether the increase in 4C DNA is causally related to the accumulation of ␤-tubulin, whether it is restricted to the radicle tip, whether it leads to cell division only after radicle protrusion, and whether this is species dependent remain to be clarified.…”

mentioning

confidence: 99%

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Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not

Castro

Lammeren

Groot³

et al. 2000

Plant Physiology

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131

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We studied cell cycle events in embryos of tomato (Lycopersicon esculentum Mill. cv Moneymaker) seeds during imbibition in water and during osmoconditioning ("priming") using both quantitative and cytological analysis of DNA synthesis and ␤-tubulin accumulation. Most embryonic nuclei of dry, untreated control seeds were arrested in the G 1 phase of the cell cycle. This indicated the absence of DNA synthesis (the S-phase), as confirmed by the absence of bromodeoxyuridine incorporation. In addition, ␤-tubulin was not detected on western blots and microtubules were not present. During imbibition in water, DNA synthesis was activated in the radicle tip and then spread toward the cotyledons, resulting in an increase in the number of nuclei in G 2 . Concomitantly, ␤-tubulin accumulated and was assembled into microtubular cytoskeleton networks. Both of these cell cycle events preceded cell expansion and division and subsequent growth of the radicle through the seed coat. The activation of DNA synthesis and the formation of microtubular cytoskeleton networks were also observed throughout the embryo when seeds were osmoconditioned. However, this preactivation of the cell cycle appeared to become arrested in the G 2 phase since no mitosis was observed. The pre-activation of cell cycle events in osmoconditioned seeds appeared to be correlated with enhanced germination performance during re-imbibition in water.

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supporting

confidence: 84%

Section: Dna Synthesis and Appearance Of Microtubule Arrays Is Correlmentioning

confidence: 93%

mentioning

confidence: 99%

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Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not

Castro

Lammeren

Groot³

et al. 2000

Plant Physiology

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131

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“…Considerable experimental evidence compels the view that resumption of cell cycle activity is a specific feature of early germination (Georgieva et al, 1994;Gó rnik et al, 1997;Ö zbingö l et al, 1999;de Castro et al, 2000;Vázquez-Ramos, 2000). Our previous work (Gallardo et al, 2001) and the present study revealed an accumulation of five proteins associated with cell cycle events during germination of the WT Arabidopsis seeds.…”

Section: Cell Cycle Activitymentioning

confidence: 99%

Proteomics of Arabidopsis Seed Germination. A Comparative Study of Wild-Type and Gibberellin-Deficient Seeds

et al. 2002

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We examined the role of gibberellins (GAs) in germination of Arabidopsis seeds by a proteomic approach. For that purpose, we used two systems. The first system consisted of seeds of the GA-deficient ga1 mutant, and the second corresponded to wild-type seeds incubated in paclobutrazol, a specific GA biosynthesis inhibitor. With both systems, radicle protrusion was strictly dependent on exogenous GAs. The proteomic analysis indicated that GAs do not participate in many processes involved in germination sensu stricto (prior to radicle protrusion), as, for example, the initial mobilization of seed protein and lipid reserves. Out of 46 protein changes detected during germination sensu stricto (1 d of incubation on water), only one, corresponding to the cytoskeleton component ␣-2,4 tubulin, appeared to depend on the action of GAs. An increase in this protein spot was noted for the wild-type seeds but not for the ga1 seeds incubated for 1 d on water. In contrast, GAs appeared to be involved, directly or indirectly, in controlling the abundance of several proteins associated with radicle protrusion. This is the case for two isoforms of S-adenosyl-methionine (Ado-Met) synthetase, which catalyzes the formation of Ado-Met from Met and ATP. Owing to the housekeeping functions of Ado-Met, this event is presumably required for germination and seedling establishment, and might represent a major metabolic control of seedling establishment. GAs can also play a role in controlling the abundance of a ␤-glucosidase, which might be involved in the embryo cell wall loosening needed for cell elongation and radicle extension.Maturation drying is the normal terminal event in the vast majority of seeds, after which they pass into a metabolically quiescent state where they may remain for many years and still retain their viability (Hoekstra et al., 2001). Upon hydration under suitable conditions, the seed, if not dormant, reactivates its metabolism and commences germination, giving rise to a new plant.Seed germination can be divided into three phases, imbibition, increased metabolic activity, and initiation of growth, which loosely parallel the triphasic water uptake of dry mature seeds. Morphologically, initiation of growth corresponds to radicle emergence; subsequent growth is generally defined as seedling growth. By definition, germination sensu stricto incorporates those events that start with the uptake of water by the quiescent dry seed and terminate with the protrusion of the radicle and the elongation of the embryonic axis (Bewley, 1997). From physiological studies on a wide variety of species, including a number of mutants, it appears that gibberellins (GAs) play a key role in late stages of seed germination Karssen, 1988, 1992;Karssen et al., 1989;Hilhorst and Toorop, 1997; Yamaguchi et al., 1998; Richards et al., 2001). Thus, in plant species such as Arabidopsis and tomato (Lycopersicon esculentum), the strong alleles of GA-deficient mutants are unable to complete germination without exogenous GAs (Koornneef and van der Veen,...

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“…Posteriormente, a síntese de DNA ocorre durante a Fase S (síntese) e por último a Fase G2, na qual os núcleos assumem conteúdo de DNA equivalentes a 4C, indicando a ocorrência de replicação de DNA em preparação para mitose (Castro e Hilhorst, 2000). Em suma, o alongamento da radícula dentro da semente ocorre como resultado da embebição e reativação do metabolismo e do ciclo celular, podendo envolver tanto expansão como divisão celular (Górnik, 1997;Castro e Hilhorst, 2004;Marcos Filho, 2005), o que por sua vez pode estar vinculado à maior sensibilidade à dessecação ou perda de tolerância à dessecação durante o progresso do processo germinativo.…”

Section: Introductionunclassified

Avaliação da perda da tolerância à dessecação e da quantidade de dna nuclear em sementes de Peltophorum dubium (spreng.) taubert durante e após a germinação

Guimarães¹,

Faria²,

Oliveira³

et al. 2011

Rev. bras. sementes

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RESUMO -O presente trabalho teve por objetivo a avaliação da perda da tolerância à dessecação (TD) em sementes de peltophorum dubium durante e após a germinação. As sementes foram colocadas para germinar e, ao atingirem 1, 3 e 5 mm de raiz primária (68% de umidade), foram desidratadas em sílica gel, até atingirem o grau de umidade inicial (8%), sendo em seguida reidratadas e avaliadas quanto à sobrevivência (retomada do crescimento e formação de plântulas normais). Procedimento semelhante foi adotado para os ensaios realizados durante a embebição, onde foram amostradas 100 sementes divididas em quatro repetições de 25 para cada um dos seguintes tempos de embebição: 12, 24, 48, 60 e 72 horas. Em seguida, foram selecionados diferentes pontos de interesse (12, 48 e 60 horas de embebição e raízes primárias com 1 mm de comprimento) para determinação da quantidade de DNA nuclear, afim de analisar possível correlação entre início do ciclo celular e perda da TD. Com relação ao comportamento de sementes germinadas submetidas à secagem e reidratação, para os três comprimentos de raízes primárias amostradas, não houve sobrevivência. Foi observada queda progressiva na sobrevivência de sementes de peltophorum dubium relacionada ao tempo de embebição, e posterior secagem e reidratação, sugerindo que a perda da TD desta espécie acontece nos estágios iniciais da germinação, antes da protrusão da radícula. Sementes embebidas por 12 h, 24 h, 48 h, 60 h, 72 h e aquelas germinadas, com 1 mm de comprimento radicular, apresentaram índices iguais a 98%, 93%, 83%, 35%, 17% e 0% de sobrevivência respectivamente. Os estudos relacionados ao conteúdo de DNA nuclear não demonstram correlação entre retomada do ciclo celular e perda da TD.Termos para indexação: DNA nuclear, ciclo celular, peltophorum dubium. EVALUATION OF THE LOSS OF DESICCATION TOLERANCE AND NUCLEAR DNA CONTENT IN SEEDS OF peltophorum dubium (SPRENG.) TAUBERT DURING AND AFTER GERMINATIONABSTRACT -The objective of this study was to investigate the loss of desiccation tolerance (DT) in seeds of peltophorum dubium (Sprengel) Taubert, during and after germination. p. dubium seeds were sown, removed after germination when the primary root lengths reached 1, 3 and 5 mm

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Inhibition of cell division during cabbage (Brassica oleraceaL.) seed germination

Cited by 36 publications

References 16 publications

Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not

Cell Division and Subsequent Radicle Protrusion in Tomato Seeds Are Inhibited by Osmotic Stress But DNA Synthesis and Formation of Microtubular Cytoskeleton Are Not

Proteomics of Arabidopsis Seed Germination. A Comparative Study of Wild-Type and Gibberellin-Deficient Seeds

Avaliação da perda da tolerância à dessecação e da quantidade de dna nuclear em sementes de Peltophorum dubium (spreng.) taubert durante e após a germinação

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