Inhibition of Cell Fusion by Neutralizing Monoclonal Antibodies to F1 Protein of Turkey Rhinotracheitis Virus.

Tanaka, Masayuki; Obi, Takeshi; Takuma, Hiroshi; Kokumai, Norihide; Higashihara, Rika; Hiramatsu, Kazuhisa; Shimizu, Yukio

doi:10.1292/jvms.58.581

Cited by 2 publications

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“…Two MAbs (1C1F8 and 2C2E9) did not show neutralizing activity, while one MAb (1C1F8) did not show fusion inhibition activity. Our findings are similar to the results obtained for human respiratory syncytial virus (HRSV) and bovine respiratory syncytial virus (BRSV) in relation to the inhibitory activity for fusion and neutralization (Matheise et al, 1995;Tanaka et al, 1996). Protein F probably plays an important role in this case.…”

Section: Resultssupporting

confidence: 90%

“…Protein F probably plays an important role in this case. It is suggested that the neutralization epitopes are the same as or very similar to the fusion-active site on protein F (Tanaka et al, 1996). The two major antigens of aMPV are the F (fusion) and the G (attachment) proteins.…”

Section: Resultsmentioning

confidence: 99%

“…In Japan, MAbs were used to investigate the antigenic relationship among strains of aMPV. That study demonstrates that the Japanese strains present antigenic differences from British isolates (Tanaka et al, 1996;Obi et al, 1997). In the present study, we produced MAbs against the SHS-BR-121 strain, and used them to compare the SHS-BR-121 strain with two aMPV strains isolated in Germany by cross-neutralization test.…”

Section: Introductionmentioning

confidence: 92%

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Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil

Coswig

Stach-Machado

Arns

2007

Rev. Bras. Cienc. Avic.

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Avian Metapneumovirus (aMPV), also called Turkey Rhinotracheitis Virus (TRTV), is an upper respiratory tract infection of turkeys, chickens and other avian species. Five monoclonal antibodies (MAbs) were created against the Brazilian isolate (SHS-BR-121) of aMPV, MAbs 1A5B8; 1C1C4; 2C2E9 and 2A4C3 of IgG1 and MAb 1C1F8 of IgG2a. Four Mabs (1A5B8; 1C1C4; 2C2E9 and 2A4C3) showed neutralizing activity and three (1A5B8; 1C1C4 and 2A4C3) inhibited cellular fusion in vitro. These MAbs were used to investigate antigenic relationship among three strains (SHS-BR-121, STG 854/88 and TRT 1439/91) of aMPV subtypes A and B using cross-neutralization test. The results confirm that the monoclonal antibodies described can be used as a valuable tool in the epizootiological and serological studies, and also for the specific diagnosis of the subtypes in the infection for Avian Metapneumovirus.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 92%

See 1 more Smart Citation

Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil

Coswig

Stach-Machado

Arns

2007

Rev. Bras. Cienc. Avic.

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“…In the spring of 1994, we first isolated TRTV from commercial broiler chickens with SHS in Miyazaki prefecture, and designated this agent 8597/CV94 [19]. The antigenic properties of the 8597/ CV94 strain of TRTV have previously been reported using monoclonal antibodies (mAbs) to fusion (F; F1) and matrix (M) proteins [21].…”

Section: Virusesmentioning

confidence: 99%

“…Serial twofold dilutions of polyclonal antiserum or purified mAb showing neutralization activities (previously reported mAbs Pn2-2E and Pn3-2F [21] and Pn01-8E, Pn06-4D) were mixed with 200 TCID 50 of each virus. Antigenic similarities among the TRTV strains examined by polyclonal guinea pig antisera were formulated as described by Archetti and Horsfall [1] (formula; r 1 × r 2 , on condition that r n = heterologous neutralization titer/homologous neutralization titer).…”

Section: Virusesmentioning

confidence: 99%

Antigenic Differentiation of Turkey Rhinotracheitis Virus Strains Using Monoclonal Antibodies and Polyclonal Antisera.

Obi

Kokumai

Ibuki

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Monoclonal antibodies (mAbs) were prepared against the 8597/CV94 strain of turkey rhinotracheitis virus (TRTV). These mAbs were used to investigate antigenic relationships among three strains (8597/CV94, 1162/92 and CVL14/1 strain) of TRTV, together with polyclonal chicken and rabbit antisera to 8597/CV94 strain, and guinea pig antisera to each of the three strains. Thirty mAbs to the glycoprotein (G:3 clones), fusion (F1:6 clones), phosphorylated (P:6 clones), nucleocapsid (N:12 clones), and matrix (M:3 clones) proteins of viral antigen were obtained by cell fusion. Among these, two mAbs to F1 protein showed virus neutralizing activity. The results of ELISA test indicated that some mAbs only reacted to the 8597/CV94 strain, some reacted to 8597/CV94 and 1162/92 strains, and others reacted to all three viral strains. In neutralization tests with the three virus strains, polyclonal chicken and rabbit antisera against the 8597/ CV94 strain showed the same antibody titers. Results with four neutralizing mAbs including two previously reported mAbs [Ref. 21] indicated the titers of two mAbs (Pn2-2E and Pn3-2F) to 8597/CV94 were much higher than those to the other two viral strains. No differences were observed in the titers of the other two mAbs (Pn01-8E and Pn06-4D) against any viral strains. In cross-neutralization tests with polyclonal guinea pig antisera, there was some variations among viral strains. This work demonstrated that the Japanese isolate 8597/CV94 of TRTV is somewhat different in antigenicity from two British isolates from chickens and turkeys. strains isolated in the UK from chickens and turkeys by enzyme-linked immunosorbent assay (ELISA) and virus neutralization tests with the newly produced mAbs and polyclonal antisera. MATERIALS AND METHODS Viruses:The virus used in this study was 8597/CV94 strain isolated from chickens with SHS. British strains, 1162/92 isolated from chickens [10] and CVL14/1 isolated from turkeys [24], were supplied by Dr. R. E. Gough (Central Veterinary Laboratory, UK). The viruses were propagated in Vero cells, and purified from infected culture fluid by differential ultracentrifugation and by sedimentation through a 20-60% discontinuous sucrose gradient at 100,000 ×g for 90 min. The virus band was collected and used as purified virus for immunization and the ELISA antigens.Antisera: Guinea pigs were immunized with the three strains of TRTV. Specific pathogen free chickens and rabbits were immunized with the 8597/CV94 strain. The virus suspensions containing 1.0 × 10 6.0 TCID 50 /ml emulsified with an equal amount of Freund's complete or incomplete adjuvant were employed as immunogens. Rabbits and guinea pigs were injected intramuscularly and then subcutaneously at two-week intervals with 1 or 0.5 ml of the virus. Chickens were given 0.1 ml of the virus suspension without adjuvant intraocularly twice at two-week intervals. The animals were bled two weeks after the last injection, and the sera were stored at 20°C until use.

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Inhibition of Cell Fusion by Neutralizing Monoclonal Antibodies to F1 Protein of Turkey Rhinotracheitis Virus.

Cited by 2 publications

References 0 publications

Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil

Production of monoclonal antibodies for Avian Metapneumovirus (SHS-BR-121) isolated in Brazil

Antigenic Differentiation of Turkey Rhinotracheitis Virus Strains Using Monoclonal Antibodies and Polyclonal Antisera.

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